›› 2009, Vol. 52 ›› Issue (3): 301-311.

• 研究论文 • 上一篇    下一篇


郭亭亭, 姜辉, 高希武   

  • 出版日期:2009-04-14 发布日期:2009-03-20
  • 通讯作者: 高希武

Diversity, evolution and expression regulation of cytochrome P450 monooxygenase genes in insects

  • Online:2009-04-14 Published:2009-03-20

摘要: 细胞色素P450单加氧酶(cytochrome P450 monooxygenases, P450s)是由多个功能相关的亚铁血红素硫醇盐蛋白基因组成的一个基因超家族, 在各种内源和外源物质的代谢中起着主要作用。目前GenBank中注册的昆虫P450基因序列已超过1 000个, 其中双翅目占序列总数的74%, 鳞翅目占序列总数的16%。而昆虫P450基因序列已克隆的全长序列中大部分属于CYP4和CYP6家族, 两个家族成员分别占总数的20%和45%。利用GenBank中现已注册的昆虫P450基因的cDNA全长序列进行比对并绘制进化树, 揭示不同种类昆虫P450的亲缘关系。结果显示基于P450基因的昆虫部分目的进化关系与大部分先前依据其他分子数据或形态分类学得到的昆虫系统进化关系基本吻合。现有研究表明, 细胞色素P450基因的表达可能受顺式作用元件(cis-acting element)、反式作用因子(trans-acting factor)或两者共同调控, 调控可能涉及转录增强的转录机制或mRNA稳定性增加的转录后机制。

关键词: 细胞色素P450, 多样性, 亲缘关系, 基因表达与调控

Abstract: Cytochrome P450 monooxygenases(P450s) are a superfamily of genes encoding multifunctional heme-thiolate proteins that play a dominant role in the metabolism of a wide variety of both endogenous and xenobiotic substances. The total number of P450 genes in insects registered in the GenBank is over 1 000. Most of the registered P450 gene sequences are from Diptera (74%) and Lepidoptera (16%). Of the total full-length sequences of P450 genes of insects registered, 20% are CYP6 members and 45% CYP4 members. A novel phylogenetic tree of the insect P450 superfamily which expounds evolutionary relationship of different subfamilies and orders was here constructed using a multiple alignment of the registered insect P450 sequences. The relationships of insect orders revealed by the phylogenetic tree most match the evolutionary relationships of insect orders based on other molecular data or morphological taxonomy. The present research indicates that the expression of cytochrome P450 genes may be regulated by cis-acting element or/and trans-acting factor, and the regulation mechanisms may involve the transcription enhancement (transcriptional mechanism) or an increase in mRNA stability (post-transcriptional mechanism).

Key words: Cytochrome P450, diversity, evolutionary relationship, gene expression and regulation