关键词: 葡萄根瘤蚜, 葡萄, 寄生选择性, 气质联用, 挥发性化合物

Abstract: To probe into either the attractiveness to or avoidance from phylloxera of grape rootstocks and cultivars with different tolerance, as well as into the volatile components from their roots, the method of invitro root segment culture was used to investigate the selectivity of Phylloxera viticola Fitch to the roots of grape rootstocks ‘5BB’ and ‘140Ru’ and cultivar ‘Kyoho’. The results showed great selectivity of phylloxera to ‘Kyoho’ roots relative to ‘5BB’ and ‘140Ru’. Three days after inoculation, 67.13% grape phylloxera selected ‘Kyoho’, while only 15.29% and 17.58% phylloxera selected ‘5BB’ and ‘140Ru’, respectively. The results also demonstrated significant difference in development and reproduction of grape phylloxera on the root segments of ‘Kyoho’, ‘5BB’ and ‘140Ru’. In addition, the volatile components from the roots of ‘Kyoho’ and ‘5BB’ were determined. It was found that each of them produced a large number of unique volatile components. ‘Kyoho’ produced 23 unique volatile components accounting for 48.94% of the total, while ‘5BB’ had 32 accounting for 57.14%. Among these volatile components, 24 were shared by ‘Kyoho’ and ‘5BB’, which were linoleic acid, methyl ester and elaidic acid, methyl ester with different contents. The contents of caryophyllene, linoleic acid (methyl ester), elaidic acid (methyl ester), and hexadecanoic acid (methyl ester) in 5BB roots were 4.23%−6.46％ higher than those in ‘Kyoho’ roots, while the contents of dibutyl phthalate and cholestane in ‘Kyoho’ roots were 6.81% and 1.07% higher than those in 5BB roots, respectively. The results suggest that phylloxera has different selectivity to grape rootstocks and cultivars with different tolerance, and these rootstocks and cultivars produce different volatile components.

Key words: Phylloxera viticola, grape, host selectivity, gas chromatography-mass (GC-MS), volatiles