›› 2009, Vol. 52 ›› Issue (6): 610-616.doi:

• 研究论文 • 上一篇    下一篇



  • 出版日期:2009-06-20 发布日期:2009-06-20
  • 通讯作者: 杜永均

Expression and localization analysis of general odorant binding protein 1 (GOBP1) gene in Spodoptera litura (Lepidoptera: Noctuidae)

  • Online:2009-06-20 Published:2009-06-20

摘要: 气味调控斜纹夜蛾Spodoptera litura(鳞翅目,夜蛾科)的交配和产卵行为,而嗅觉气味结合蛋白(OBP)是昆虫与外界环境进行化学信息交流中的一种重要蛋白。本研究基于已报道的斜纹夜蛾普通气味结合蛋白GOBP1基因cDNA序列(GenBank登录号为EF159978)设计引物,通过RT-PCR法分析了GOBP1 mRNA的组织特异性表达情况,并对GOBP1基因条带进行了克隆、测序和Blast比对。此外,再通过RNA原位杂交的方法进一步分析了GOBP1 mRNA在触角中的表达定位。结果表明:GOBP1只在斜纹夜蛾的触角组织中表达,而且GOBP1转录本较多分布于靠近嗅觉感受器即触角边缘部位。这些结果进一步说明GOBP1是斜纹夜蛾嗅觉过程中的重要蛋白,同时为深入研究GOBP1与其他蛋白的相互空间定位关系、GOBP1的功能等奠定了基础。

关键词: 斜纹夜蛾, 普通气味结合蛋白, 基因表达定位, 原位杂交

Abstract: Odor chemically mediates Spodoptera litura (Lepidoptera, Noctuidae) mating and oviposition behaviors, and odorant-binding protein (OBP) on the moth antenna is one of the important kinds of proteins involved in chemical communication with the environment. In this study the primers were designed according to the sequence of S. litura GOBP1 (GenBank accession no. EF159978) reported before, and the tissue expression pattern of GOBP1 in the moth was analyzed by using RT-PCR technology. The results showed that GOBP1 is an antennal-specific protein and expresses only in the antennae of S. litura. The fragments of GOBP1 gene were also cloned, sequenced and blasted against GenBank. The further experiments of localization of GOBP1’s mRNA by in situ hybridization methods revealed that the GOBP1 specifically express in the tissue of moth antenna, and their transcripts were more widely distributed in the cells bordering of the antennal surfaces where the chemosensory hairs are located. The results suggest that GOBP1 plays crucial roles in moth olfaction recognition. Our findings provide the basis for the further study on the function of GOBP1 and its interaction with spatial localization of other proteins.

Key words: Spodoptera litura, general odorant binding protein (GOBP), localization of gene expression, in situ hybridization