›› 2009, Vol. 52 ›› Issue (9): 1000-1007.doi:

• 研究论文 • 上一篇    下一篇

基于mtDNA-COI基因序列的雷氏按蚊分子群体遗传结构研究

杨曼尼, 马雅军   

  • 出版日期:2009-09-20 发布日期:2009-09-20
  • 通讯作者: 马雅军

Molecular population genetic structure of Anopheles lesteri (Diptera: Culicidae) based on mtDNA-COI gene sequences

YANG Man-Ni, MA Ya-Jun   

  • Online:2009-09-20 Published:2009-09-20

摘要: 【目的】探讨我国雷氏按蚊Anopheles lesteri的群体差异和分化程度。【方法】采用PCR方法, 从分子水平鉴别了采自我国和韩国的雷氏按蚊共9个群体139个样本, 扩增其线粒体DNA细胞色素氧化酶亚基Ⅰ基因, 并进行序列测定和分析。【结果】本研究共获得49个单倍型, 各单倍型呈高水平的平行演化, 来自云南群体的单倍型显示是扩张的源头。分子变异等级分析(AMOVA)的计算结果显示, 群体内变异占总变异的比例(64.95%)大于群体间(35.05%), FST值为0.3504, 各群体间出现遗传分化。Mantel检验结果显示基因流水平与地理距离呈负相关关系(R2=0.1322), 群体遗传结构符合距离隔离模型。【结论】雷氏按蚊韩国和辽宁群体与其他分布地群体间差异大, 已出现明显分化, 我国其他分布地群体间的遗传差异小。

关键词: 雷氏按蚊, 群体遗传结构, 线粒体DNA, 单倍型, 中国

Abstract: 【Aim】 To study the population genetic variation and the diversity level of Anopheles lesteri from China. 【Methods】 The 139 specimen of 9 populations from China and South Korea were identified by PCR assay. Mitochondrial DNA cytochrome oxidase sununit I gene was amplified, and the mtDNA-COI gene sequences were sequenced and analyzed. 【Results】 In this study, 49 haplotypes were obtained, which were at a high level of homoplasy, and the haplotypes of Yunnan population showed to be the expansion origin. The results of the analysis of molecular variance (AMOVA) showed that the percentage of variation within a population (64.95%) was greater than that among populations (35.05%). The FST value was 0.3504, and the genetic divergence among populations was significant. Mantel test results indicated that the level of gene flow was negatively correlated with geographic distances (R2=0.1322). The population genetic structure fits the isolation-by-distance model. 【Conclusion】 The greatest difference is found between the populations of South Korea and Liaoning and other populations from China, and they have exhibited obvious differentiation. The level of variation among populations except those of Liaoning and South Korea is low.

Key words: Anopheles lesteri, population genetic structure, mitochondrial DNA, haplotype, China