›› 2010, Vol. 53 ›› Issue (12): 1352-1359.

• 研究论文 • 上一篇    下一篇


姜卫华, 熊满辉, 王志田, 卢伟平, 刘萍, 郭文超, 李国清   

  • 出版日期:2011-01-17 发布日期:2010-12-20
  • 通讯作者: 李国清

Incidence and synergism of resistance to conventional insecticides in larvae of the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), in northern Xinjiang, China(In English)

JIANG Wei-Hua, XIONG Man-Hui, WANG Zhi-Tian,LU Wei-Ping, LIU Ping, GUO Wen-Chao, LI Guo-Qing   

  • Online:2011-01-17 Published:2010-12-20
  • Contact: LI Guo-Qing


为了了解并掌握马铃薯甲虫Leptinotarsa decemlineata (Say)在新疆的抗性水平及动态, 为科学用药提供理论依据, 利用点滴法测定了新疆北部马铃薯甲虫6个田间种群(乌鲁木齐、 阜康、 昌吉、 阿勒泰、 尼勒克和特克斯) 4龄幼虫对14种常规药剂的敏感性, 并测试了磷酸三苯酯(TPP)、 顺丁烯二酸二乙酯(DEM)和氧化胡椒基丁醚(PBO)的增效作用。结果表明: 与已报道的基线相比, 特克斯种群对几种药剂都敏感, 因此确定特克斯田间种群为敏感品系。与特克斯敏感品系相比, 昌吉种群对三氟氯氰菊酯、 溴氰菊酯、 克百威及丁硫克百威的抗性倍数分别为108.8, 30, 17.624.7, 而尼勒克种群对这4种药剂的抗性倍数分别为29.4, 42.5, 10.189.9。此外, 昌吉和阜康种群对高效氯氰菊酯也分别产生8.913.0倍的中等抗性。增效测定结果显示: 对于尼勒克种群, PBO对三氟氯氰菊酯有明显增效作用; 对于昌吉种群, PBO, TPPDEM对克百威有明显增效作用。因此推断解毒酶活性增强可能是新疆马铃薯甲虫对三氟氯氰菊酯和克百威的抗性机制之一。由于增效作用不能完全消除抗性, 因此新疆马铃薯甲虫的抗药性还可能涉及到其他机制。

关键词: 马铃薯甲虫, 抗药性, 点滴法, 增效剂, 增效作用, 抗性机制


To understand the resistance levels and dynamics of Colorado potato beetle, Leptinotarsa decemlineata (Say) (CPB) in Xinjiang to conventional insecticides and offer the fundamental data for res0stance management, six overwintered CPB adult populations were collected from potato fields of Urumqi, Fukang, Changji, Altay, Nilka, and Tekes counties (cities) in northern Xinjiang Uygur autonomous region in China, and the first generation of 4th instar larvae were tested for resistance to fourteen conventional insecticides by topical application. And possible resistance mechanisms were investigated using three synergists, triphenyl phosphate (TPP), diethylmeleate (DEM), and piperonyl butoxide (PBO). The results showed that the Tekes field population was susceptible to several tested insecticides when compared to the baselines that have been documented. This population, therefore, was selected as a reference susceptible strain. Compared with the Tekes strain, Changji and Nilka populations had extremely high- to high- or moderate-level resistance to cyhalothrin (resistance ratio was 108.8- and 29.4-fold, respectively), deltamethrin (30- and 42.5-fold, respectively), carbofuran (17.6 and 10.1-fold, respectively), and carbosulfan (24.7- and 89.9-fold, respectively). Moreover, the Changji and Fukang beetles developed low- to moderate-level resistance to α-cypermethrin(8.9- and 13.0-fold, respectively). Synergism studies revealed that PBO had significant synergism to cyhalothrin in the Nilka population, and PBO, TPP and DEM exhibited significant synergism to carbofuran in the Changji population. These results suggest that enzymatic detoxification may be one of the resistance mechanisms to cyhalothrin and carbofuran for local populations in Xinjiang. Because the synergists do not completely eliminate CPB resistance, there are probably additional resistance mechanisms involved.

Key words: Colorado potato beetle, insecticide resistance, topical bioassay, synergist, synergism, resistance mechanism