›› 2010, Vol. 53 ›› Issue (5): 525-530.doi:

• 研究论文 • 上一篇    下一篇

岩木瓜化学成分的分离、结构鉴定与杀虫活性

王学贵,沈丽淘,田永清,徐汉虹   

  • 出版日期:2010-05-20 发布日期:2010-05-20
  • 通讯作者: 徐汉虹

Isolation, structure identification and insecticidal activity of compounds from Ficus tsiangii Merr. ex Corner

WANG Xue-Gui, SHEN Li-Tao, TIAN Yong-Qing, XU Han-Hong   

  • Online:2010-05-20 Published:2010-05-20

摘要: 为明确岩木瓜Ficus tsiangii Merr. ex Corner的杀虫活性成分及杀虫活性,利用活性跟踪分离技术,通过硅胶柱层析、薄层层析及葡聚糖凝胶等手段对岩木瓜茎和根皮的化学成分进行了分离、纯化,结合化合物理化性质和波谱数据对其结构进行了鉴定.结果表明,从岩木瓜中分离得到4个生物活性化合物,分别为β-香树脂醇乙酸酯、蒲公英赛酮、柯依利素及齐墩果酸.利用胃毒法测定了这些化合物(500μg/g糖)对家蝇Musca domestica成虫的生物活性,其中蒲公英赛酮处理试虫48 h后的校正死亡率达到83.33%,表现出较强杀虫活性,并进一步测定了其对试虫的毒力.结果表明,蒲公英赛酮处理家蝇成虫48 h后表现出较强毒力,其LC50值为89.82μg/g糖,略低于对照药剂鱼藤酮毒力(LC50值为67.58μg/g糖).因此,蒲公英赛酮值得进一步深入研究.

关键词: 岩木瓜, 活性跟踪分离, 家蝇, 杀虫活性, 蒲公英赛酮, 生物农药

Abstract:  In this study the chemical constituents of Ficus tsiangii Merr. ex Corner stem and root-bark were isolated and puified with activity-guided fractionation technology by silica gel, thin-layer chromatography and Sephadex LH-20 column chromatography to investigate the constituents with insecticidal activity, and their structures were identified with spectroscopic means. The results indicated that four bioactive compounds were isolated and their structures were identified as β-amyrin acetate, taraxerone, chrysoeriol and oleanolic acid, respectively. The insecticidal activities of these compounds(500 μg/g sugar) were assayed against Musca domestica vicina adults with stomach-toxicity test, of which the corrected mortality achieved by taraxerone was 83.33% at 48 h after treatment, showing higher insecticidal activity. The toxicity of taraxerone against M. domestica vicina adults was also evaluated with LC50 value of 89.82 μg/g sugar at 48 h after treatment, which was only a little weaker than that of the control with rotenone (LC50 value of 67.58 μg/g sugar). Therefore taraxerone can be further investigated as a biopesticide.

Key words: Ficus tsiangii, activity-guided fractionation, Musca domestica, insecticidal activity, taraxerone, biopesticide