›› 2010, Vol. 53 ›› Issue (5): 531-539.doi:

• 研究论文 • 上一篇    下一篇

褐飞虱侵害后不同水稻品种根及叶片脱落酸含量的变化

刘井兰,吴进才,于建飞,杨国庆   

  • 出版日期:2010-05-20 发布日期:2010-05-20
  • 通讯作者: 吴进才

Changes in levels of abscisic acid in roots and leaves of different rice varieties under infestation by Nilaparvata lugens (Stål) (Homoptera: Delphacidae)

LIU Jing-Lan, WU Jin-Cai, YU Jian-Fei, YANG Guo-Qing   

  • Online:2010-05-20 Published:2010-05-20

摘要: 为了解褐飞虱Nilaparvata lugens (Stål)侵害后水稻耐虫性与植物体内源激素关系,应用酶联免疫吸附法(enzyme-linked immunosorbent assay, ELISA)研究褐飞虱若虫侵害分蘖期超级培矮64S/E32和TN1,灌浆期协优963和TN1后根及叶片脱落酸(abscisic acid, ABA)含量变化.结果表明:褐飞虱侵害分蘖期超级培矮64S/E32和TN1后3 d,叶片ABA含量显著上升,ABA含量根冠比(根ABA/叶片ABA)显著下降;侵害后6 d,超级培矮64S/E32叶片ABA含量显著下降,根冠比显著上升;但TN1叶片ABA含量在褐飞虱侵害后3 d和6 d显著上升,根冠比显著下降.褐飞虱侵害灌浆期协优963与分蘖期超级培矮64S/E32变化一致,TN1在褐飞虱侵害后3 d叶片ABA含量显著上升,根冠比显著下降;侵害后6 d,叶片ABA含量、ABA含量根冠比均显著上升.由ABA含量变化百分比可见,分蘖期ABA含量变化幅度较灌浆期大;耐虫品种变化幅度较感虫品种大,持续期较感虫品种短;叶片变化幅度较根部大.褐飞虱侵害后,两种不同生育期两种抗性不同的水稻品种比较,耐虫品种叶片ABA含量先上升(3 d)后下降(6 d),ABA含量根冠比先下降(3 d)后上升(6 d);感虫品种叶片ABA含量持续上升(3 d和6 d),分蘖期ABA含量根冠比持续下降(3 d和6 d),灌浆期ABA含量根冠比先下降(3 d)后上升(6 d);耐、感虫水稻品种根部变化规律不明显.这些差别表明不同水稻(耐虫和感虫)品种受褐飞虱侵害后体内ABA含量变化规律不同.本研究结果对深入阐明水稻耐虫品种的机制具有重要参考价值.

关键词: 褐飞虱, 水稻品种, 植物抗性, 根, 叶片, 脱落酸含量

Abstract: To understand the relationship between rice tolerance and endogenous plant hormones under Nilaparvata lugens infestation, the response of abscisic acid (ABA) in roots and leaves at the tillering and grain-filling stages of representative rice varieties (super-high yield rice Peiai 64S/E32,Xieyou 963 and TN1)to N. lugens nymph infestation was studied using enzyme-linked immunosorbent assay (ELISA). The results indicated that ABA contents in leaves at 3 d after N. lugens infestation at the tillering stage for superhigh yield rice Peiai 64S/E32 and TN1 significantly increased. Ratio of ABA contents in roots to those in leaves (R∶L ratio) decreased significantly. ABA contents in leaves at 6 d after infestation for super-high yield rice Peiai 64S/E32 significantly decreased, but the R∶L ratio significantly increased. But ABA contents in leaves of TN1 significantly increased and the R∶L ratio decreased. At the grain-filling stage, change of ABA for Xieyou 963 was similar to Peiai 64S/E32 at the tillering stage. For TN1, ABA contents in leaves at 3 d after infestation increased and the R∶L ratio decreased. ABA contents in leaves at 6 d after infestation for 40 nymphs per plant and the R∶L ratio increased. Percent of increase or decrease of ABA contents showed that change of ABA contents at the tillering stage was greater than that at the grain-filling stage, and that for tolerant variety was greater than that for susceptible rice variety, but the effective duration was shorter. The change of ABA contents in leaves was greater than that in roots. Comparison of change of ABA contents of the two rice varieties showed that, for tolerant rice variety, ABA contents in leaves at 3 d after infestation increased and those at 6 d after infestation decreased, and the R∶L ratio at 3 d after infestation decreased, that at 6 d after infestation increased; while for the susceptible rice variety, ABA contents in leaves at 3 d and 6 d after infestation increased and the R∶L ratio decreased at the tillering stage at 3 d and 6 d. At the grain-filling stage, the R∶L ratio at 3 d after infestation decreased while that at 6 d after infestation increased. The change of ABA contents in roots after N. lugens infestation was not distinct. The results demonstrated that the effect of N. lugens on ABA contents in plants varied with rice variety (tolerant and susceptible). The findings have scientific value for ascertaining the tolerance mechanism of rice variety.

Key words: Nilaparvata lugens, rice variety, plant resistance, roots, leaves, abscisic acid (ABA) content