三种杀虫剂对褐飞虱海藻糖含量和海藻糖酶活性的影响

›› 2011, Vol. 54 ›› Issue (7): 786-792.doi:

三种杀虫剂对褐飞虱海藻糖含量和海藻糖酶活性的影响

赵克非, 戈林泉, 程耀, 吴进才, 杨国庆

收稿日期:2010-11-20 出版日期:2011-07-20 发布日期:2011-07-20
作者简介:赵克非，男， 1986年生，河南郏县人，硕士研究生，主要从事水稻害虫再猖獗的研究， E-mail: zhaokefei128@126.com
基金资助:
国家“973”计划项目(2010CB126200)；国家自然科学基金项目(30870393)

Effects of three insecticides on trehalose content and trehalase activity in the brown planthopper, Nilaparvata lugens (Stål）(Homoptera: Delphacidae)

ZHAO Ke-Fei, GE Lin-Quan, CHENG Yao, WU Jin-Cai, YANG Guo-Qing

Received:2010-11-20 Online:2011-07-20 Published:2011-07-20
About author:zhaokefei128@126.com

摘要/Abstract

摘要： 为了解农药处理导致褐飞虱Nilaparvata lugens (Stål）飞行能力增强的生理机制，本文采用蒽酮法和酶促反应终止法，研究了吡虫啉、三唑磷和溴氰菊酯3种杀虫剂亚致死剂量对褐飞虱3龄、 5龄若虫及长、短翅型雌雄成虫体内海藻糖含量和海藻糖酶活性的影响。结果表明：杀虫剂处理的褐飞虱3龄若虫海藻糖含量和海藻糖酶活性与对照相比没有显著差异（P>0.05）。40 mg/L三唑磷处理的褐飞虱5龄若虫体内海藻糖含量显著低于对照（P<0.05），比对照降低了24%；而20和40 mg/L三唑磷处理的褐飞虱5龄若虫海藻糖酶活性显著高于对照（P<0.05），分别比对照高出了100%和129%。10 mg/L吡虫啉， 20 和40 mg/L三唑磷以及3和6 mg/L溴氰菊酯处理的褐飞虱短翅雌成虫和雄成虫体内海藻糖含量显著低于对照（P<0.05），雌成虫体内海藻糖含量比对照分别降低了36%， 53%， 67%， 58%和69%，雄成虫体内海藻糖含量比对照分别降低了59%， 71%， 65%， 70%和77%；而40 mg/L三唑磷以及3和6 mg/L溴氰菊酯处理的褐飞虱短翅型雌成虫和雄成虫体内海藻糖酶活性显著高于对照（P<0.05），雌成虫体内海藻糖酶活性比对照分别高出了124%， 100%和88%，雄成虫体内海藻糖酶活性比对照分别高出了146%， 132%和118%。10 mg/L吡虫啉， 40 mg/L三唑磷和3 mg/L溴氰菊酯处理的褐飞虱长翅型雌成虫和雄成虫海藻糖含量显著低于对照（P<0.05），雌成虫海藻糖含量比对照分别降低了44%， 34%和37%，雄成虫体内海藻糖含量比对照降低了48%， 54%和43%；而5和10 mg/L吡虫啉处理的长翅型雌成虫和雄成虫海藻糖酶活性显著高于对照（P<0.05），雌成虫体内海藻糖酶活性比对照分别高出了317%和300%，雄成虫体内海藻糖酶活性比对照分别高出了170%和97%。这些结果说明这3种杀虫剂亚致死剂量处理可以增强褐飞虱体内海藻糖酶活性，并导致海藻糖含量下降。本研究结果对深入阐明农药诱导褐飞虱再猖獗及杀虫剂处理增强其飞行能力的生理机制具有一定的科学价值。

关键词: 褐飞虱, 杀虫剂, 亚致死剂量, 海藻糖, 海藻糖酶, 能量物质, 飞行能力

Abstract: To understand the physiological mechanism of insecticide-induced enhancement of flight capacity of the brown planthopper, Nilaparvata lugens (Stål), we examined the changes of trehalose content and the trehalase activity in the 3rd and 5th instar nymphs and the adults of N. lugens under the stress of sublethal dose of three insecticides (imidacloprid, triazophos and deltamethrin).The results showed that the trehalose content and the trehalase activity in the 3rd instar nymphs treated with three insecticides were not significantly different from those of the control (P>0.05). The trehalose content in the 5th instar nymphs treated with 40 mg/L triazophos was significantly lower than that in the control (P<0.05), decreased by 24%, while the trehalase activity in the 5th instar nymphs treated with 20 and 40 mg/L triazophos was significantly higher than that in the control (P<0.05), increased by 100% and 129%, respectively. Compared to the controls, the trehalose content in brachypterous female and male adults was significantly reduced in treatments of 10 mg/L imidacloprid, 20 and 40 mg/L triazophos, and 3 and 6 mg/L deltamethrin (P<0.05), decreased by 36%, 53%, 67%, 58% and 69% in female adults, and 59%, 71%, 65%, 70% and 77% in male adults, respectively. However, the trehalase activity in brachypterous female and male adults was significantly increased in treatments of 40 mg/L triazophos and 3 and 6 mg/L deltamethrin, compared to the controls (P<0.05), increased by 124%, 100% and 88% in female adults, and 146%, 132% and 118% in male adults, respectively. The trehalose content in macropterous female and male adults treated with 10 mg/L imidacloprid, 40 mg/L triazophos and 3 mg/L deltamethrin was significantly lower than that in the control (P<0.05), decreased by 44%, 34% and 37% in female adults, and 48%, 54% and 43% in male adults, respectively. However, the trehalase activity in macropterous female and male adults treated with 5 and 10 mg/L imidacloprid, was significantly higher than that in the control (P<0.05), increased by 170% and 97% in female adults, and 317% and 300% in male adults, respectively. The results suggest that the trehalase activity of N. lugens is increased by treatments of sublethal dose of the three insecticides, while the trehalose content is reduced. The present work provides some valuable clues for understanding the mechanism of insecticide-induced enhancement of flight capacity of the N. lugens.

Key words: Nilaparvata lugens, insecticide, sublethal dose, trehalose, trehalase, energy substance, flight capacity

赵克非, 戈林泉, 程耀, 吴进才, 杨国庆. 三种杀虫剂对褐飞虱海藻糖含量和海藻糖酶活性的影响[J]. , 2011, 54(7): 786-792.

ZHAO Ke-Fei, GE Lin-Quan, CHENG Yao, WU Jin-Cai, YANG Guo-Qing. Effects of three insecticides on trehalose content and trehalase activity in the brown planthopper, Nilaparvata lugens (Stål）(Homoptera: Delphacidae)[J]. , 2011, 54(7): 786-792.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/

http://www.insect.org.cn/CN/Y2011/V54/I7/786

[1]	王佳, 樊欢, 熊克才, 刘映红. 柑橘大实蝇滞育和非滞育蛹体内海藻糖含量的调控[J]. , 2018, 61(9): 1010-1018.
[2]	卢绍辉, 魏美才, 袁国军, 崔建新, 梅象信. 悬铃木方翅网蝽飞行能力测试及远距离扩散轨迹分析[J]. , 2018, 61(9): 1097-1105.
[3]	袁锐, 李丽莉, 李超, 夏小菊, 郑礼, 于毅, 门兴元. 六种新烟碱类杀虫剂对凹唇壁蜂的毒性及风险评估[J]. , 2018, 61(8): 950-956.
[4]	刘瑞莹, 肖子衿, 贺静澜, 万贵钧, 潘卫东, 陈法军. 迁飞性害虫褐飞虱对地磁强度变化的种群适合度响应[J]. , 2018, 61(8): 957-967.
[5]	王德意, 汝玉涛, 王勇, 马月月, 那爽, 孙良振, 姜义仁, 秦利. 柞蚕蛹滞育和滞育解除过程中海藻糖酶基因表达模式及酶活力分析[J]. , 2018, 61(7): 784-794.
[6]	艾东, 林荣华, 王猛, 梁晓贺, 于彩虹. 棉铃虫可溶型海藻糖酶的化学修饰[J]. , 2018, 61(7): 801-807.
[7]	陈龙, 周晓榕, 高利军, 谭瑶, 庞保平. 沙葱萤叶甲成虫越夏期间糖类、蛋白及脂肪含量的变化[J]. , 2018, 61(7): 808-814.
[8]	冯娅琳, 郝培应, 俞飞飞, 陆潮峰, 朱家骏, 俞晓平. 褐飞虱ATP合酶b亚基基因ATPSb的克隆与功能分析[J]. , 2018, 61(5): 519-526.
[9]	陈龙, 谭瑶, 周晓榕, 庞保平, 格希格都仁, 孙志鹏. 沙葱萤叶甲海藻糖酶基因GdTre1的克隆、分子特性和表达分析[J]. , 2018, 61(3): 271-281.
[10]	Fahimeh HAMIDI, Ali MEHRVAR, Naser EIVAZIAN KARY, Hassan VALIZADEH. 百里香、欧芹和熏衣草水苏提取物对四纹豆象成虫的生物活性(英文)[J]. , 2018, 61(3): 331-339.
[11]	高景鹏, 陈翰明, 马雅军. 我国白纹伊蚊对三种拟除虫菊酯类杀虫剂抗性检测诊断剂量的建立[J]. , 2018, 61(1): 18-24.
[12]	邹金城, 孙宇, 杨勇, 杨益众, 张子昕, 王思雨, 苏宏华. 镉胁迫显著影响甜菜夜蛾成虫的飞行能力[J]. , 2017, 60(9): 1021-1030.
[13]	朱文雅, 吴海花, 张学尧, 刘娇, 张建珍, 马恩波. 飞蝗细胞色素P450基因LmCYP6FD3的表达及其在杀虫剂解毒中的作用[J]. , 2017, 60(7): 742-749.
[14]	苗雨桐, 邓瑶, 刘玉娣, 侯茂林. 褐飞虱成虫唾液细菌蛋白的鉴定[J]. , 2017, 60(7): 772-781.
[15]	周浩, 邵莒南, 翟一凡, 吴光安, 陈浩, 门兴元, 于毅, 郑礼. 氟啶虫胺腈、乙基多杀菌素及其混剂对地熊蜂的毒性及风险评估[J]. , 2017, 60(7): 809-816.