›› 2011, Vol. 54 ›› Issue (7): 793-799.doi:

• 研究论文 • 上一篇    下一篇

市售长叶松油和锡兰肉桂油对登革热媒蚊埃及伊蚊的灭幼效果(英文)

 Radhika WARIKOO, Naim WAHAB, Sarita KUMAR   

  • 收稿日期:2010-08-27 出版日期:2011-07-20 发布日期:2011-07-20
  • 作者简介:sarita.sanjay90@gmail.com

Larvicidal potential of commercially available pine (Pinus longifolia) and cinnamon (Cinnamomum zeylanicum) oils against dengue fever mosquito, Aedes aegypti L.(Diptera: Culicidae)

Radhika WARIKOO, Naim WAHAB, Sarita KUMAR   

  • Received:2010-08-27 Online:2011-07-20 Published:2011-07-20
  • About author:sarita.sanjay90@gmail.com

摘要: 松油和桂皮油由于具有芳香性气味, 因而成为良好的成虫驱避剂, 但是关于它们对蚊虫的杀幼虫作用研究不多。为揭示市售的长叶松Pinus longifolia油和锡兰肉桂Cinnamomum zeylanicum油对来源于印度德里的埃及伊蚊Aedes aegypti 4龄幼虫的毒杀潜力, 我们进行了室内研究, 以幼虫死亡率及行为改变和形态改变等指标评估其杀幼虫潜力。结果表明: 两种油对埃及伊蚊4龄幼虫均具有毒杀作用, 且松油的灭幼效果优于桂皮油。松油的LC50和LC70 值分别为0.33093 mg/L 和0.54476 mg/L, 而桂皮油的LC50和LC70 值分别为0.63159 mg/L和0.77736。进一步观察发现, LC90剂量下桂皮油的杀幼虫潜力强于松油, 其LC90为1.11879 mg/L, 而松油的LC90为 1.04915 mg/L。在处理的幼虫中观察到行为改变, 如兴奋、 坐立不安、 颤抖、 痉挛然后瘫痪, 说明这两种油可能对其神经肌肉系统产生了影响。显微观察处理幼虫的形态改变发现, 与对照相比, 大多数器官的外观正常, 只是肛鳃略为内收缩而引起结构畸形, 提示肛腮可能是这两种油的作用位点, 腮的功能异常引起了幼虫死亡。这两种油品可开发用作防治蚊虫的新型杀幼虫药剂。

关键词: 埃及伊蚊, 松树, 肉桂, 杀幼虫潜力, 肛腮, 痉挛

Abstract: The aromatic nature of pine and cinnamon oils has established them as good adult repellents but their larvicidal efficacy against mosquitoes has not been explored much. Keeping this in view, laboratory studies were conducted to uncover the larvicidal potential of commercially available pine (Pinus longifolia) oil and cinnamon (Cinnamomum zeylanicum) oil against the early 4th instar larvae of Aedes aegypti, originating from Delhi, India. The larvicidal effects were investigated in terms of the larval mortality, behavioural and morphological changes, if any. Our studies revealed the larvicidal efficiency of both the oils against 4th instar larvae of Ae. aegypti, the pine oil proving to be more effective than the cinnamon oil. The LC50 and LC70 values exhibited by pine oil were 0.33093 mg/L and 0.54476 mg/L, respectively, while the values obtained with cinnamon oil were 0.63159 mg/L and 0.77736 mg/L, respectively. It was further observed that at LC90 the larvicidal potential of cinnamon oil surpassed that of the pine oil, exhibiting a value of 1.11879 mg/L as in comparison to 1.04915 mg/L shown by pine oil. The behavioural changes as excitation, restlessness, tremors, and convulsions followed by paralysis observed in the treated larvae suggest a probable effect of the oils on their neuromuscular system. Microscopic study of morphological alterations in the treated larvae revealed that most of their organs had a normal structural appearance as that of controls except the little internal shrinkage in anal gills leading to the structural deformity. This indicates the anal gills as the probable action sites of the oil extracts and dysfunction of the gills leading to larval mortality. The potential of oils as new types of larvicides for the control of mosquitoes are explored.

Key words: Aedes aegypti, pine, cinnamon, larvicidal potential, anal gills, convulsions