›› 2011, Vol. 54 ›› Issue (7): 828-837.doi:

• 研究论文 • 上一篇    下一篇

基于线粒体COⅡ基因序列的双斑长跗萤叶甲中国北方地理种群的遗传多样性研究

 梁日霞, 王振营, 何康来, 丛斌, 李菁   

  • 收稿日期:2011-02-10 出版日期:2011-07-20 发布日期:2011-07-20
  • 作者简介:梁日霞, 女, 1984年生, 山西省山阴县人, 硕士研究生, 动物细胞与分子生物学, E-mail: lrxlscx@163.com
  • 基金资助:

    现代农业产业技术体系建设专项(CARS-02);  公益性行业(农业)科研专项(200903004)

Genetic diversity of geographic populations of Monolepta hieroglyphica (Motschulsky) (Coleoptera: Chrysomelidae) from North China estimated by mitochondrial COⅡ gene sequences

LIANG Ri-Xia, WANG Zhen-Ying, HE Kang-Lai, CONG Bin, LI Jing   

  • Received:2011-02-10 Online:2011-07-20 Published:2011-07-20
  • About author:lrxlscx@163.com

摘要: 双斑长跗萤叶甲Monolepta hieroglyphica (Motschulsky)是自2001年以来在我国北方为害玉米呈加重趋势的一种害虫。为初步探讨中国北方不同地理种群间和种群内该害虫的遗传分化程度、 遗传多样性以及基因流水平, 对来自中国北方的26个不同地理种群的线粒体COⅡ (细胞色素C氧化酶亚基Ⅱ) 基因片段序列的核苷酸多态性进行了研究。结果表明: 在515头个体的长度为484 bp COⅡ片段中共发现了28个变异位点和15种单倍型。单倍型间的系统进化分析发现, 15种单倍型主要分为两大分支。总种群单倍型多样性指数Hd为0.257, 种群内单倍型多样度在0.100~0.515范围内。总种群的Fst为0.585, Gst为0.417, 基因流Nm为0.35。AMOVA分子变异分析结果发现, 双斑长跗萤叶甲的遗传分化主要来自种群之间, 占方差比率的58.58%。实验总种群及大部分种群的中性检验符合中性突变, 说明我国北方双斑长跗萤叶甲在近期没有出现种群扩张现象。研究结果揭示中国北方双斑长跗萤叶甲不同地理种群间基因流水平低, 种群间已发生明显的遗传分化, 分化主要来自种群之间。

关键词: 双斑长跗萤叶甲, 线粒体 COⅡ, 地理种群, 遗传多样性, 遗传分化

Abstract: Monolepta hieroglyphica (Motschulsky) (Coleoptera: Chrysomelidae) has become more serious on maize in some maize planting areas of North China since 2001. To investigate the level of genetic diversity, genetic differentiation and gene flow among different populations and within populations of M. hieroglyphica in North China, partial sequences (484 bp) of mtDNA COⅡ (mitochondrial cytochrome oxidase subunit Ⅱ) gene in 26 geographic populations from North China were sequenced and analyzed. In the total 515 individuals, 28 variable sites and 15 haplotypes were observed. Phylogenetic tree of 15 COⅡ haplotypes exhibited two main clusters. The mean haplotype diversity of all populations was 0.257, varied from 0.100 to 0.515 within populations. The overall Fst value was 0.585, Gst was 0.417, and the average gene flow (Nm) among the 26 populations was 0.35. Molecular variance (AMOVA) analysis indicated that a high proportion of the total genetic variance was attributable to variations among populations (58.58%). The Tajima’s D neutrality test results suggest that for M. hieroglyphica in North China there might be no population expansion. These results indicate that the level of gene flow among different populations is very low,  and there is obvious differentiation among M. hieroglyphica populations in North China.

Key words: Monolepta hieroglyphica, mtDNA COⅡ, geographic population, genetic diversity, genetic differentiation