›› 2012, Vol. 55 ›› Issue (4): 376-385.doi:

• 研究论文 • 上一篇    下一篇

花蓟马雄虫释放的聚集信息素的分离和鉴定

祝晓云, 张蓬军, 吕要斌   

  1. 南京农业大学植物保护学院, 南京 210095
  • 收稿日期:2011-10-07 修回日期:2012-04-11 出版日期:2012-04-20 发布日期:2012-04-20
  • 通讯作者: 吕要斌 E-mail:luybcn@163.com
  • 作者简介: 祝晓云,女,1985年10月生,山东青岛人,硕士研究生,主要从事生理生态学研究,E-mail: wannengzhu@126.com
  • 基金资助:

    国家公益性行业科研专项(201103026); 浙江省“重中之重”学科“现代农业生物技术与作物病害防控”开放基金(2010KFJJ007)

Isolation and identification of the aggregation pheromone released by male adults of Frankliniella intonsa (Thysanoptera: Thripidae)

ZHU Xiao-Yun, ZHANG Peng-Jun, Lu Yao-Bin   

  1. Department of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2011-10-07 Revised:2012-04-11 Online:2012-04-20 Published:2012-04-20
  • Contact: Lu Yao-Bin E-mail:luybcn@163.com
  • About author:wannengzhu@126.com

摘要: 明确花蓟马Frankliniella intonsa雄虫释放的聚集信息素对于花蓟马生物防治具有重要意义。但目前除了西花蓟马F. occidentalis, 关于其他蓟马雄虫释放的聚集信息素及其成分鉴定的研究未有报道。本研究采用Y型嗅觉仪测定花蓟马雌雄成虫和西花蓟马雌雄成虫对花蓟马雄虫和西花蓟马雄虫气味源的嗅觉反应; 用固相微萃取(solid-phase microextraction,SPME)方法提取花蓟马雄虫挥发物, 利用含有BGB-176 SE或CP-chirasil-Dex CB 2种不同手性柱的气-质联用仪(gas chromatography-mass spectrometry, GC-MS)对挥发物进行分离和鉴定。行为学试验结果表明: 花蓟马雄虫气味源对其雄性及雌性成虫均具有明显的吸引作用。经GC-MS分析, 花蓟马雄虫挥发物中含有2种主要的化合物, 分别为(R)-lavandulyl acetate和neryl (S)-2-methylbutanoate, 其与已报道的西花蓟马雄虫释放的聚集信息素成分相同。但定量分析结果表明, (R)-lavandulyl acetate与neryl (S)-2-methylbutanoate在花蓟马和西花蓟马雄虫中释放的比例不同; 行为学试验进一步证明, 2种组分比例的不同在花蓟马和西花蓟马种间识别过程中发挥重要作用。本研究推测花蓟马属的昆虫释放的聚集信息素组分可能是相同的。

关键词: 花蓟马, 聚集信息素, (R)-lavandulyl acetate, neryl (S)-2-methylbutanoate, 嗅觉反应, 气味源, 挥发物

Abstract: Identification of aggregation pheromone released by Frankliniella intonsa males play a key role in bio-control of this pest. Except for the western flower thrips Frankliniella occidentalis, little is understood on aggregation pheromones in other thrips species. In the laboratory we tested the behavioral responses of adult F. intonsa and F. occidentalis to odor sources emitted from their corresponding adult males with Y-tube olfactometer. Head-space volatiles released by adult males of F. intonsa were collected using solid-phase microextraction (SPME) and identified by gas chromatography-mass spectrometry (GC-MS) with different GC columns (BGB-176 SE and CP-chirasil-Dex CB). Behavioral results showed that both male and female F. intonsa were attracted to the odors released by conspecific males. GC-MS analysis showed that two major components in head-space volatiles were released by F. intonsa, i.e., (R)-lavandulyl acetate and neryl (S)-2-methylbutanoate, which are the same compounds that are reported in volatiles from F. occidentalis males. However, quantitative analysis showed that the ratios of (R)-lavandulyl acetate to neryl(S)-2-methylbutanoate released by F. intonsa and F. occidentalis adults differed. Behavioral experiments further showed that the difference in the ratio of the two components plays an important role in interspecies identification between F. intonsa and F. occidentalis. The results suggest that different species of thrips of the same genus Frankliniella may share the same aggregation pheromones.

Key words: Frankliniella intonsa, aggregation pheromone, (R)-lavandulyl acetate, neryl (S)-2-methylbutanoate, olfactory response, odor sources, volatiles

中图分类号: 

  • Q968