›› 2012, Vol. 55 ›› Issue (9): 1062-1068.doi:

• 研究论文 • 上一篇    下一篇

薇甘菊甲醇提取物对二疣犀甲生长发育的影响

钟宝珠, 吕朝军, 王东明, 李洪, 覃伟权   

  • 收稿日期:2012-07-03 修回日期:2012-09-03 出版日期:2012-09-20 发布日期:2012-09-20
  • 通讯作者: 吕朝军 E-mail:lcj5783@126.com
  • 作者简介:钟宝珠, 女, 1981年生, 河北唐山人, 硕士, 助理研究员, 研究方向为害虫生物防治, E-mail: baozhuz@163.com

Effects of methanol extracts of Mikania micrantha on the growth and development of the rhinoceros beetle, Oryctes rhinoceros (Coleoptera: Dynastidae)

ZHONG Bao-Zhu,  Lu Chao-Jun , WANG Dong-Ming, LI Hong, QIN Wei-Quan   

  • Received:2012-07-03 Revised:2012-09-03 Online:2012-09-20 Published:2012-09-20
  • Contact: Lu Chao-Jun E-mail:lcj5783@126.com
  • About author:baozhuz@163.com

摘要: 【目的】探讨入侵杂草薇甘菊Mikania micrantha的利用价值, 及其在棕榈害虫二疣犀甲Oryctes rhinoceros生态防控中的应用前景。【方法】采用浸渍法和室内饲喂法, 研究了薇甘菊甲醇提取物对二疣犀甲取食量、 卵孵化、 化蛹、 羽化及幼虫发育等的影响。【结果】薇甘菊提取物对二疣犀甲具有很好的生长发育调节活性。拒食活性研究结果表明, 在不同供试浓度下, 其对二疣犀甲3龄幼虫均表现拒食活性, 且拒食率与处理浓度呈正相关。薇甘菊提取物处理二疣犀甲的卵后, 孵化率明显降低, 且孵化期延长, 10 mg/mL提取物处理后孵化率仅达66.66%, 孵化期比对照延迟3 d, 同时初孵幼虫死亡率也高达40.43%。采用添加薇甘菊提取物的饲料饲喂1龄幼虫后, 幼虫体重增长减缓, 在处理浓度为10和5 mg/g时, 处理后90 d体重增加量分别为3.83 g和4.53 g, 而对照组体重增加量达到6.87 g。薇甘菊提取物处理老熟幼虫后, 对其化蛹具有抑制作用, 造成化蛹率降低, 化蛹时间延长及蛹的畸形。经薇甘菊提取物处理二疣犀甲蛹后, 成虫羽化率降低, 羽化时间明显延长, 且畸形成虫数量增加, 主要表现为翅无法正常伸展、 虫体瘦小、 无法正常爬行等。【结论】薇甘菊具有开发为生物源昆虫生长调节剂、 并在二疣犀甲防控中应用的潜力。

关键词: 二疣犀甲, 薇甘菊, 提取物, 生长, 发育, 生物活性

Abstract: 【Aim】 To explore the potential of Mikania micrantha on ecological control of the rhinoceros beetle, Oryctes rhinoceros. 【Methods】 The effects of methanol extracts of M. micrantha on O. rhinoceros was studied using dipping method and feeding method. 【Results】 M. micrantha extracts showed good activity on feeding amount, hatchability, pupation, emergency and larval development of O. rhinoceros. M. micrantha extracts had antifeedant activity against the 3rd larvae of O. rhinoceros, with a positive correlation between antifeedant rate and the concentration of extracts. The egg hatchability decreased significantly and the hatching time was delayed after O. rhinoceros was treated with M. micrantha extracts, with the hatchability of 66.66% and the hatching time being prolonged 3 d compared with the control. The mortality was 40.43% when the 1st instar larvae hatched from eggs treated with 10 mg/mL extracts. The body weight gain of the larvae was also restrained by the M. micrantha extracts, being 3.83 g and 4.53 g at 90 d after the M. micrantha extracts were added to the diets at the concentrations of 10 mg/mL and 5 mg/mL, respectively, while in the blank, the body weight gain was 6.87 g. M. micrantha extracts also inhibited the pupation of O. rhinoceros. The pupation time was delayed, and the number of deformed pupae increased after the larvae were treated with the M. micrantha extracts. M. micrantha extracts also decreased the emergence of O. rhinoceros and prolonged the emergence time. The deformity of the treated adults was increased, manifested as deformed wings, smaller body size, disorder in walking, etc. 【Conclusion】 M. micrantha extracts show good prospects to be exploited as botanical insect growth regulators and can be applied in O. rhinoceros control.

Key words: Oryctes rhinoceros; Mikania micrantha, extracts, growth, development, bioactivity