抗吡虫啉棉蚜种群对啶虫脒和噻虫胺的交互抗性及相关酶学机理

›› 2013, Vol. 56 ›› Issue (10): 1143-1151.doi:

抗吡虫啉棉蚜种群对啶虫脒和噻虫胺的交互抗性及相关酶学机理

陈小坤，夏晓明，王红艳，乔康，王开运

（1. 山东农业大学植物保护学院，山东泰安 271018； 2. 山东省棉花研究中心，济南 250100）

出版日期:2013-10-20 发布日期:2013-10-20

Cross-resistance to clothianidin and acetamiprid in the imidacloprid-resistant strain of Aphis gossypii (Hemiptera: Aphididae) and the related enzyme mechanisms

CHEN Xiao-Kun¹， XIA Xiao-Ming¹， WANG Hong-Yan²， QIAO Kang¹， WANG Kai-YUN^1,*

(1. College of Plant Protection, Shandong Agricultural University, Tai’an, Shandong 271018, China; 2. Cotton Research Center of Shandong Province, Jinan 250100, China)

Online:2013-10-20 Published:2013-10-20

摘要/Abstract

摘要： 【目的】通过对乙酰胆碱受体β1亚基突变后的抗吡虫啉棉蚜Aphis gossypii (Glover)种群的继续筛选，明确该种群的抗性发展规律以及对其他新烟碱类杀虫剂啶虫脒和噻虫胺的交互抗性及相关酶学机理。【方法】采用浸渍法连续对抗吡虫啉棉蚜进行室内筛选、测定噻虫胺和啶虫脒对抗吡虫啉棉蚜种群的毒力；选择LC₂₀剂量吡虫啉、啶虫脒和噻虫胺处理抗性棉蚜，采用生化分析法测定其体内羧酸酯酶、谷胱甘肽-S-转移酶和乙酰胆碱酯酶的活性变化，并观察其生物学特性的变化。【结果】本研究对抗性棉蚜突变种群用吡虫啉继续筛选至75代，抗性倍数达到72.6倍， RF75停止用药筛选12代（RF₇₅₊₁₂），抗性仍达72.0倍。且RF₇₅₊₁₂对噻虫胺和啶虫脒的交互抗性可分别达11.9倍和20.1倍。噻虫胺对抗吡虫啉棉蚜的蜜露分泌和体重的抑制作用均大于吡虫啉和啶虫脒。噻虫胺对RF₇₅₊₁₂的羧酸酯酶、谷胱甘肽-S-转移酶和乙酰胆碱酯酶均具有明显的抑制作用，而啶虫脒的抑制作用较小。【结论】结果表明乙酰胆碱受体基因突变棉蚜种群对吡虫啉的抗性水平不仅升高，且停止用药后其抗性可稳定遗传；第二代新烟碱类的噻虫胺在抗吡虫啉棉蚜靶标突变种群的治理中具有较大的应用价值。

关键词: , 棉蚜, 吡虫啉, 噻虫胺, 抗性, 交互抗性, 酶学机理

Abstract: 【Aim】 To clarify the development of resistance to imidacloprid and the related enzyme mechanism of cross-resistance to other neonicotinoids, clothianidin and acetamiprid, the imidacloprid-resistant strain of the contton aphid, Aphis gossypii, was continuously selected in the laboratory after we found a mutation in nicotinic acetylcholine receptor (nAChR) β1 subunit. 【Methods】 Leaf dipping method was used to select the imidaclopridresistant strain of A. gossypii and to test the cross-resistance to acetamiprid and clothianidin. The LC₂₀ of acetamiprid and clothianidin were applied to the imidacloprid-resistant strain, and the effects on activities detoxifying enzymes and biological characteristics were determined with biochemical method and the systematic observation, respectively. 【Results】 The resistant strain of A. gossypii was continuously selected with imidacloprid for 75 generations and the resistance ratio reached to 72.6-fold as high as that of the susceptible strain. When resistance selection was suspended in the next successive 12 generations, the resistance of aphids just decreased to 72.0-fold as high as that of the susceptible strain. By generation F₇₅₊₁₂, the strain showed 11.9-fold and 20.1-fold cross-resistance to acetamiprid and clothianidin, respectively. Clothianidin could significantly inhibit the activities of CarE, GST and AChE of the imidacloprid-resistant cotton aphid, while acetamiprid showed little effect on their activities. Clothianidin had stronger inhibition on honeydew excretion and bodyweight gain of A. gossypii than other two insecticides. 【Conclusion】 The results demonstrate that the imidacloprid-resistant mutation strain not only has high-level resistance to imidacloprid, but also the resistance can be stably inherited. Therefore clothianidin has a great application value in control of the imidacloprid-resistant mutation strain of cotton aphid.

Key words: Aphis gossypii, imidacloprid, clothianidin, resistance, cross-resistance, enzyme mechanism

陈小坤，夏晓明，王红艳，乔康，王开运. 抗吡虫啉棉蚜种群对啶虫脒和噻虫胺的交互抗性及相关酶学机理[J]. , 2013, 56(10): 1143-1151.

CHEN Xiao-Kun， XIA Xiao-Ming， WANG Hong-Yan， QIAO Kang， WANG Kai-YUN . Cross-resistance to clothianidin and acetamiprid in the imidacloprid-resistant strain of Aphis gossypii (Hemiptera: Aphididae) and the related enzyme mechanisms[J]. , 2013, 56(10): 1143-1151.

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