›› 2013, Vol. 56 ›› Issue (2): 212-218.doi:

• 简报 • 上一篇    


李朝品1,2,*, 姜玉新2, 刘婷2, 郭伟2, 王少圣2, 陈琪2   

  1. (1. 安徽理工大学医学院, 安徽淮南232001; 2. 皖南医学院医学寄生虫学教研室, 安徽芜湖241002)
  • 出版日期:2013-02-20 发布日期:2013-02-20

SEM observation on external morphology of Caloglyphus berlesei (Acari: Astigmata: Acaridae) at different developmental stages

LI Chao-Pin1,2,*, JIANG Yu-Xin2, LIU Ting2, GUO Wei2, WANG Shao-Sheng2, CHEN Qi2   

  1. (1. School of Medicine, Anhui University of Science and Technology, Huainan, Anhui 232001, China; 2. Department of Medical Parasitology, Wannan Medical College, Wuhu, Anhui 241002, China)
  • Online:2013-02-20 Published:2013-02-20

摘要:  【目的】 观察伯氏嗜木螨Caloglyphus berlesei生活史各发育阶段外部形态和超微形态特征。【方法】 采集中华地鳖Eupolyphaga sinensis培养床上的培养料, 分离伯氏嗜木螨。将分离获得的伯氏嗜木螨双蒸水洗涤后, 一部分用于制备常规玻片标本, 光镜下直接观察; 另一部分用2.5%戊二醛溶液固定, 70%酒精再次洗涤, 临界点干燥, 置于扫描电镜下观察不同发育阶段(包括卵、 幼螨、 若螨及成螨)的超微形态特征。【结果】 电镜下伯氏嗜木螨各发育阶段的足、 刚毛和外生殖器及其附属结构的形态均清晰可辨。幼螨足3对, 足上无叶状刚毛, 基节干发达; 若螨足4对, 出现第4背毛, 生殖区发育不全; 休眠体足爪和前跗节发达, 出现叶状毛、 胫节毛及膝节毛等结构, 生殖板骨化明显, 其两侧有吸盘和刚毛各1对。吸盘板上共有4对成对的吸盘、 1个单吸盘和2对类圆形微凸。成螨生殖感觉器骨化且呈心形, 雄雌成螨生殖感觉器的刚毛数量上有明显差异。【结论】 对伯氏嗜木螨形态和超微形态特征的观察有助于对其进一步科学分类和进行生活史研究, 并可为控制伯氏嗜木螨及其引起的过敏性疾病提供参考。

关键词: 伯氏嗜木螨, 形态学, 超微形态, 休眠体, 吸盘板, 扫描电镜

Abstract:  【Aim】 To observe the morphological changes of the live Caloglyphus berlesei and the ultra-structure under scanning electron microscope (SEM) at different developmental stages. 【Methods】 C. berlesei specimens were isolated from the bed feedsduff in an Eupolyphaga sinensis breeding farm, and rinsed with double distilled water. One portion of the isolations were used for slide preparation in the conventional manner and immediately observed under light microscope, and the other was fixed with 2.5% glutaraldehyde solution and washed once again with 75% alcohol. By critical-point drying, the specimens were observed under SEM for the ultrastructure characteristics at different developmental stages including egg, larva, nymph and adult. 【Results】 Morphological variations of C. berlesei, including its legs, setae, external genitalia and accessories, are clearly identified under SEM. The larva has three pairs of legs, without leaf-like setae, yet its coxal rod is well-developed. By nymphal stage, four pairs of legs and the 4th dorsal seta arise, whereas the genital area looks still under-developed. At hypopus, the claws and tarsules are well-built, and leaf-like setae, setae of tibia and setae of genu are seen. One pair of suckers and setas are located on both sides of the genital plates which are ossified obviously. There are 4 pairs of suckers, single sucker and 2 pairs of round-like bulge in sucker plate. The genital sense organ of adults exhibits itself with cordiform external aspect and typical ossification texture, wherease the male is dissimilar with the female regarding seta number on the genital sense organ. 【Conclusion】 Description of the morphology and ultrastructure of C. berlesei provides important information for the taxonomy and further study of its life history as well as basis for controlling the allergic disorders due to infection of this pest.

Key words: Caloglyphus berlesei, morphology, ultrastructure, hypopus, sucker plate, scanning electron microscopy (SEM)