›› 2013, Vol. 56 ›› Issue (3): 243-250.doi:

• 研究论文 • 上一篇    下一篇


葛星1,2, 张天涛2, 何康来2, 王勤英1, 李云龙3, 王振营2   

  1. (1. 河北农业大学植物保护学院,  河北保定 071000; 2. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室,
     北京 100193; 3. 北京市植物保护站, 北京 100032)
  • 出版日期:2013-03-20 发布日期:2013-03-20

Cloning and tissue expression profiling of the olfactory receptor co-receptor gene in adults of Conogethes punctiferalis (Lepidoptera: Crambidae)

GE Xing1,2, ZHANG Tian-Tao2, HE Kang-Lai2, WANG Qin-Ying1, LI Yun-Long3, WANG Zhen-Ying2,*   

  1. (1. College of Plant Protection, Agricultural University of Hebei, Baoding, Hebei 071000, China; 2. State Key Laboratory for the Biology of Plant Diseases and Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3. Plant Protection Station of Beijing, Beijing 100032, China)
  • Online:2013-03-20 Published:2013-03-20

摘要: 【目的】克隆桃蛀螟Conogethes punctiferalis (Guenée)的Orco嗅觉受体基因, 并研究其在不同组织的表达谱。【方法】利用PCR技术克隆桃蛀螟触角Orco基因, 对该基因编码的氨基酸序列进行生物信息学分析, 并利用荧光定量PCR技术分析该基因在的表达量。【结果】获得桃蛀螟成虫Orco的cDNA全长序列, 并命名为CpunOrco(GenBank登录号: JX101681)。该基因的开放阅读框全长1 425 bp, 编码475个氨基酸, 序列中有7个跨膜区。对桃蛀螟成虫不同组织中CpunOrco的荧光定量PCR结果表明, CpunOrco主要在触角和下颚须中表达, 雄虫触角中的表达量高于雌虫, 并且该基因在其他组织中也有一定的表达。【结论】本研究明确了该嗅觉受体基因在桃蛀螟成虫不同组织内的表达水平, 为进一步研究其功能提供了理论依据。

关键词: 桃蛀螟, 嗅觉受体蛋白, 基因克隆, 荧光定量PCR, 组织表达谱

Abstract: 【Aim】 To clone the olfactory receptor co-receptor (Orco) gene in the yellow peach moth Congethes punctiferalis (Guenée) and analyze its expression level in different tissues. 【Methods】 The Orco gene was cloned by polymerase chain reaction (PCR), and its expression level was analyzed by real-time quantitative PCR. 【Results】 The Orco gene was cloned from adults of C. punctiferalis, and named as CpunOrco (GenBank accession no. JX101681). Sequence analysis revealed that the open reading frame of CpunOrco is 1 425 bp in length, encoding 475 amino acid residues, with seven transmembrane domains. Real-time quantitative PCR results indicated that CpunOrco was mainly expressed in the antennae and maxillary palpi of C. punctiferalis, while expressed in other tissues at a relatively low level. The expression level of CpunOrco was higher in antennae of male adults than in antennae of female adults. 【Conclusion】 This study makes clear the expression level of CpunOrco in different tissues of C. punctiferalis adults and provides a basis for further functional study of this gene.

Key words: Congethes punctiferalis, olfactory receptor co-receptor, gene cloning, real-time quantitative PCR, tissue expression profile