外源茉莉酸诱导的青杨叶片保护性酶活性变化及其对舞毒蛾幼虫生长发育的影响

›› 2013, Vol. 56 ›› Issue (3): 270-275.doi:

外源茉莉酸诱导的青杨叶片保护性酶活性变化及其对舞毒蛾幼虫生长发育的影响

越慧芳¹, 段立清^1,2,*, 李海平²，张丽娜²，王晓丽¹，张志林³

（1. 内蒙古农业大学林学院, 呼和浩特 010019; 2. 内蒙古农业大学农学院, 呼和浩特 010019;
3. 乌兰察布市森林病虫防治检疫站, 内蒙古乌兰察布012000)

出版日期:2013-03-20 发布日期:2013-03-20

Changes in activities of protective enzymes in green poplar induced by exogenous jasmonic acid and the effects on larval development of the gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae)

YUE Hui-Fang¹, DUAN Li-Qing^1,2,*, LI Hai-Ping², ZHANG Li-Na², WANG Xiao-Li¹, ZHANG Zhi-Lin³

(1. College of Forestry, Inner Mongolia Agricultural University, Huhhot 010019, China; 2. College of Agriculture, Inner Mongolia Agricultural University, Huhhot 010019, China; 3. Ulanqab Forestry Pests Control and Quarantine Station, Ulanqab, Inner Mongolia 012000, China)
Abstract:

Online:2013-03-20 Published:2013-03-20

摘要/Abstract

摘要： 为探讨外源茉莉酸对青杨Populus cathayana的诱导抗性及其对舞毒蛾Lymantria dispar的影响，室内对青杨扦插苗喷施不同浓度的茉莉酸（对照为0.17%丙酮），分别在喷施后1， 5和10 d采集叶片分析其保护性酶活性的变化，并接舞毒蛾幼虫于青杨苗木上观测其长发育情况。结果表明： 0.1 和0.001 mmol/L两种浓度的茉莉酸（JA）处理均使青杨叶过氧化物酶（POD）、多酚氧化酶（PPO）、苯丙氨酸解氨酶（PAL）、胰凝乳蛋白酶抑制剂（CI）和胰蛋白酶抑制剂（TI）活性较对照增加(P<0.05)。取食茉莉酸诱导的青杨苗木后，舞毒蛾幼虫的发育历期延长，体重降低。0.1 mmol/L茉莉酸诱导的青杨苗木5 d后接虫，使舞毒蛾幼虫的发育历期显著延长，较对照长8 d；接虫21 d后称重时，取食茉莉酸诱导的青杨叶片的幼虫体重较对照组降低了50%~100%，该结果说明外源茉莉酸诱导青杨产生了对舞毒蛾明显的抗虫性。

关键词: 青杨, 舞毒蛾, 诱导抗性, 茉莉酸, 保护性酶, 抗虫性

Abstract: In order to investigate the resistance induced by exogenous jasmonic acid in Populus cathayana and the effects on development of the gypsy moth, Lymantria dispar, two concentrations of jasmonic acid solution (0.1 and 0.001 mmol/L) were sprayed onto green poplar seedlings while the control was sprayed with 0.17% acetone, the change in activities of protective enzymes in treated leaves was tested at 1, 5, and 10 d post JA treatment, and the developmental duration and body weight of L. dispar larvae on them were measured. The results showed that the activities of peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), chymotrypsin inhibitor (CI) and trypsin inhibitor (TI) in leaves treated with two concentrations of jasmonic acid were significantly higher than that of the control (P<0.05). The developmental duration of L. dispar larvae fed on JA-induced green poplar seedlings was prolonged, while their body weight was reduced. The developmental duration of L. dispar larvae fed on 0.1 mmol/L JA-induced seedlings was 8 d longer than that of the control. The larval body weight of L. dispar measured at 21 d post JA treatment was decreased by 50%-100%. The results suggest that exogenous jasmonic acid could induce resistance in green poplar to L. dispar larvae.

Key words: Populus cathayana, Lymantria dispar, induced resistance, jasmonic acid, protective enzymes, insect resistance

越慧芳, 段立清, 李海平, 张丽娜, 王晓丽, 张志林. 外源茉莉酸诱导的青杨叶片保护性酶活性变化及其对舞毒蛾幼虫生长发育的影响[J]. , 2013, 56(3): 270-275.

YUE Hui-Fang, DUAN Li-Qing, LI Hai-Ping, ZHANG Li-Na, WANG Xiao-Li, ZHANG Zhi-Lin. Changes in activities of protective enzymes in green poplar induced by exogenous jasmonic acid and the effects on larval development of the gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae)[J]. , 2013, 56(3): 270-275.

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