›› 2013, Vol. 56 ›› Issue (4): 350-357.doi:

• 研究论文 • 上一篇    下一篇

家蚕复眼突变系光泽眼(lu)和光泽小眼(ve)的复眼形态观察

李琼艳, 刘春*, 荀利杰, 李栋, 党增强, 吕金凤, 夏庆友   

  1. (家蚕基因组生物学国家重点实验室, 西南大学, 重庆 400716)
  • 出版日期:2013-04-20 发布日期:2013-04-20

Morphological observation of compound eyes in the mutant strains, lustrous (lu) and varnished eye (ve), of the silkworm (Bombyx mori)

LI Qiong-Yan, LIU Chun*, XUN Li-Jie, LI Dong, DANG Zeng-Qiang, LÜ Jin-Feng, XIA Qing-You   

  1.  (State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China)
  • Online:2013-04-20 Published:2013-04-20

摘要: 家蚕Bombyx mori复眼突变系光泽眼(lustrous, lu)及光泽小眼(varnished eye, ve)都是由单基因控制的隐性突变, 目前为止, 其突变基因及突变机理还未知。为了了解其复眼突变性状内外部形态结构差异, 本研究以家蚕正常品系大造Dazao (Dz)为对照, 利用光学显微镜及扫描电镜对家蚕Dz, luve的成虫复眼和幼虫单眼表面进行观察, 并利用石蜡切片HE染色技术对3个品系复眼内部结构进行观察。结果表明: 突变体luve的复眼表面形态除了典型的富有光泽外, 复眼形状、 大小和小眼形态、 排列及数量上都与正常型明显不同。突变体lu和ve的角膜、 晶锥、 感杆束及色素细胞均发生了异常。luve不仅是复眼表面形态发生了变化, 其内部结构也发生了很大的变化。本研究为lu和ve突变基因的克隆及突变机理的阐明提供了参考信息。

关键词: 家蚕, 突变系, 复眼, 小眼, 单眼, 形态

Abstract: The lustrous (lu) and varnished eye (ve) are recessive single-gene mutants in the silkworm, Bombyx mori. So far, the mechanisms of the mutation and mutant genes are unknown. Using the wild-type strain Dazao (Dz) as the wild-type control, we observed the surface morphology of adult compound eyes and larval ocelli in lu and ve by optical microscopy and scanning electron microscopy, and the internal structures with paraffin sections and HE staining technology. The results showed that the surface morphology of the compound eyes in lu and ve except the typical characteristic of luster are significantly different from the normal, including the shape and size, and the shape, arrangement and quantities of ommatidia in lu and ve are also different from the control. The cornea, crystalline cone, rhabdom and pigment cell structures are abnormal, but not the same in lu and ve. Not only the surface of compound eyes has changed, but their internal structure has varied largely in lu and ve. This study provides helpful data for cloning mutant genes from lu and ve and clarifying the mechanisms of the mutations.

Key words: Bombyx mori, mutant strain, phenotype, compound eye, ommatidia, ocelli, morphology