低温驯化后水椰八角铁甲生理活性物质含量的变化

摘要/Abstract

摘要： 【目的】分析低温驯化后水椰八角铁甲Octodonta nipae体内各种生理活性物质含量的变化，为揭示水椰八角铁甲耐寒机制提供理论基础。【方法】以25℃处理为对照，将水椰八角铁甲各虫态于12.5, 15, 17.5和20℃驯化处理10 d后，比较其体内的游离水、蛋白质、氨基酸、粗脂肪、甘油和可溶性糖原等生理活性物质含量的变化。【结果】低温驯化显著影响铁甲体内上述物质的含量水平。低温驯化能提高该铁甲体内甘油和游离氨基酸的含量，降低游离水、蛋白质、粗脂肪、可溶性糖原的含量。与对照相比，12.5℃驯化对该铁甲各虫态体内游离水含量存在显著影响(P<0.01)；经12.5, 15, 17.5和20.0℃驯化后，该铁甲各虫态体内蛋白质、粗脂肪含量与对照相比均存在显著差异(P<0.01)；经125，150和175℃驯化后，该铁甲各虫态体内游离氨基酸含量与对照存在显著差异(P<0.01)；经12.5，15.0，17.5和20℃驯化后，该铁甲各虫态（2龄幼虫除外）体内甘油含量与对照相比存在显著差异(P<0.01)；12.5, 15.0, 17.5和20℃驯化对除2龄幼虫、蛹和成虫外的铁甲其他各虫态体内可溶性糖原含量存在显著影响。经15.0℃驯化处理后铁甲各虫态体内平均甘油含量最高，比对照25℃的平均甘油含量高出约9.4倍，而经12.5℃驯化处理的虫体内的平均甘油含量仅增加3.5倍。【结论】低温驯化对水椰八角铁甲体内相关生理活性物质含量的影响效能是有限的；水椰八角铁甲可以根据不良的环境条件调节最佳的生理状况，以适应未来的环境和达到最强的适应性。

关键词: 水椰八角铁甲, 低温驯化, 生理活性物质, 含水量, 蛋白质, 氨基酸, 脂肪, 甘油, 糖原

Abstract: 【Aim】 The effect of low-temperature acclimation on the contents of related physiologically active substances was determined in Octodonta nipae (Coleoptera: Chrysomelidae) to reveal its cold tolerance mechanisms. 【Methods】 We compared the contents of different physiological substances such as free water, protein, amino acids, crude fat, glycerol and soluble glycogen between the non-acclimation (25℃) and acclimation (acclimated at 12.5, 15.0, 17.5 and 20.0℃ for 10 d) treated O. nipae. 【Results】 The contents of related physiologically active substances in O. nipae were significantly affected by acclimation temperatures. With the decrease of the acclimation temperature, the contents of glycerol and amino acids increased, while those of the free water, protein, crude fat and glycogen decreased. Compared with the control, the contents of free water in O. nipae subjected to acclimation at 12.5℃ had significant changes (P<0.01). The contents of protein and crude fat significantly changed when O. nipae was subjected to low-temperature acclimation at 12.5, 15.0, 17.5 and 20.0℃, so did the content of the amino acids except under acclimation at 20℃ (P<0.01). Low temperature acclimation-mediated effects on glycerol content were found in O. nipae at different developmental stages except the 2nd instar larvae. Significant changes in the contents of soluble glycogen were determined in different developmental stages except the 2nd instar larvae, pupae and adults after being acclimated at 12.5, 15.0, 17.5 and 20℃, respectively. The average glycerol content in O. nipae after being acclimated at 15.0℃ was about 9.4 times higher than that at 25℃. However, after O. nipae was acclimated at 12.5℃, its average glycerol content increased only 3.5 times as compared with the control. 【Conclusion】 These results indicated that the effect of low-temperature acclimation on the contents of related physiologically active substances in O. nipae was limited. O. nipae could make the optimal physiological adjustments based on the adverse environment conditions to adapt the future environment and maximize their fitness.

Key words: Octodonta nipae, low temperature acclimation, physiologically active substances, water content, protein, amino acids, fat, glycerol, glycogen

华瑞香，侯有明，石章红. 低温驯化后水椰八角铁甲生理活性物质含量的变化[J]. , 2014, 57(3): 265-273.

HUA Rui-Xiang, HOU You-Ming, SHI Zhang-Hong . Changes in the contents of physiologically active substances in Octodonta nipae (Coleoptera: Chrysomelidae) after low temperature acclimation[J]. , 2014, 57(3): 265-273.

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低温驯化后水椰八角铁甲生理活性物质含量的变化

Changes in the contents of physiologically active substances in Octodonta nipae (Coleoptera: Chrysomelidae) after low temperature acclimation

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