棉蚜对吡虫啉的抗性选育和现实遗传力分析

›› 2014, Vol. 57 ›› Issue (3): 330-334.doi:

棉蚜对吡虫啉的抗性选育和现实遗传力分析

郭天凤^1,2，马野萍²，丁荣荣²，杜晶²，周晶²，李国萍²，蔡晓丽²，王国平²，赵富强²，李家胜²，史雪岩^1，*，高希武^1，*

(1 中国农业大学昆虫系，北京100193; 2 新疆生产建设兵团第七师农科所，新疆奎屯 833200）

出版日期:2014-03-20 发布日期:2014-03-20

Selection and realized heritability analysis of resistance to imidacloprid in cotton aphid (Aphis gossypii)

GUO Tian-Feng^1,2, MA Ye-Ping², DING Rong-Rong², DU Jing², ZHOU Jing², LI Guo-Ping², CAI Xiao-Li², WANG Guo-Ping², ZHAO Fu-Qiang², LI Jia-Sheng², SHI Xue-Yan^1，*, GAO Xi-Wu^1，*

(1. Department of Entomology, China Agricultural University, Beijing 100193, China; 2. Institute of Agricultural Sciences, Agricultural Division 7, Xinjiang Production and Construction Corps, Kuitun, Xinjiang 833200, China)

Online:2014-03-20 Published:2014-03-20

摘要/Abstract

摘要： 【目的】为了评估棉蚜Aphis gossypii Glover对吡虫啉的抗性风险，在室内进行了棉蚜对吡虫啉(imidacloprid)的抗性选育和抗性现实遗传力分析。【方法】采用单头反选育法和群体汰选法，分别得到了棉蚜对吡虫啉敏感品系(LC₅₀为0.176 mg/L)和抗性品系(LC₅₀为14.657 mg/L)。采用阈性状分析方法，获得棉蚜对吡虫啉的抗性现实遗传力（h²）。【结果】相对于田间原始种群(LC₅₀为0.346 mg/L)，吡虫啉敏感棉蚜品系对吡虫啉的LC50减少了2倍；获得的吡虫啉抗性棉蚜品系，经过40代的选育，得到抗性倍数为室内敏感品系的83.27倍的抗性品系。棉蚜对吡虫啉的抗性现实遗传力(h2)为0.1478。进一步预测其抗性发展速度，基于80%~90%的选择压力，预计抗性增长100倍时，吡虫啉可使用30.2~38.1代。【结论】这些研究说明棉蚜对吡虫啉存在抗性风险。

关键词: 棉蚜, 吡虫啉, 抗性选育, 现实遗传力, 抗性风险

Abstract: 【Aim】 In order to evaluate the resistance risk of the cotton aphid, Aphis gossypii Glover, to imidacloprid, the selection and realized heritability analysis of resistance to imidacloprid in A. gossypii were performed in the laboratory. 【Methods】 The imidaclopridsusceptible and resistant strains were developed by individual reverse and group selection, with the LC₅₀ values of 0.176 and 14.657 mg/L，respectively. The realized heritability (h²) of A. gossypii resistance to imidacloprid was estimated by Tabashnik’s method. 【Results】 Compared with the LC₅₀value of the original field strain of A. gossypii (0.346 mg/L), the LC₅₀value of the imidacloprid-susceptible strain had a 2-fold decrease. The resistance index of the imidacloprid-resistant strain was 83.27-fold as high as that of the imidacloprid-susceptible strain after 40 generations of selection. The realized heritability (h²) of A. gossypii resistance to imidacloprid was 0.1478. It was predicted that A. gossypii would require 30.2 to 38.1 generations to obtain 100-fold resistance to imidacloprid under breeding pressure of imidacloprid with 80%-90% mortality for each generation of selection. 【Conclusion】 The results suggest that A. gossypii has resistance risk to imidacloprid.

Key words: Aphis gossypii, imidacloprid, resistance selection, realized heritability, resistance risk

郭天凤，马野萍，丁荣荣，杜晶，周晶，李国萍，蔡晓丽，王国平，赵富强，李家胜，史雪岩，高希武. 棉蚜对吡虫啉的抗性选育和现实遗传力分析[J]. , 2014, 57(3): 330-334.

GUO Tian-Feng, MA Ye-Ping, DING Rong-Rong, DU Jing, ZHOU Jing, LI Guo-Ping, CAI Xiao-Li, WANG Guo-Ping, ZHAO Fu-Qiang, LI Jia-Sheng, SHI Xue-Yan, GAO Xi-Wu. Selection and realized heritability analysis of resistance to imidacloprid in cotton aphid (Aphis gossypii)[J]. , 2014, 57(3): 330-334.

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http://www.insect.org.cn/CN/Y2014/V57/I3/330

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