›› 2015, Vol. 58 ›› Issue (10): 1140-1145.

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共生菌Tremblaya princeps在扶桑绵粉蚧个体发育中的动态变化

黄芳1,2,#, 赵春玲1,3,#, 吕要斌1,3,*   

  1. (1. 浙江省农业科学院植物保护与微生物研究所, 浙江省植物有害生物防控重点实验室——省部共建国家重点实验室培育基地, 杭州 310021; 2. 湖州出入境检验检疫局, 浙江湖州 313000; 3. 浙江师范大学化学与生命科学学院, 浙江金华 321004)
  • 出版日期:2015-10-20 发布日期:2015-10-20
  • 作者简介:黄芳, 女, 1981年10月生, 浙江云和人, 博士, 副研究员, 研究方向为害虫综合防治, E-mail: huangfang_ch@hotmail.com; 赵春玲, 女, 1988年10月生, 山东菏泽人, 硕士研究生, 研究方向为害虫综合防治, E-mail: zhaochunling1026@126.com

Dynamics of Tremblaya princeps during individual development of its host Phenacoccus solenopsis (Hemiptera: Pseudococcidae)

HUANG Fang1,2,#, ZHAO Chun-Ling1,3,#, LU Yao-Bin1,3,*   

  1. (1. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2. Huzhou Entryexit Inspection and Quarantine Bureau, Huzhou, Zhejiang 313000, China; 3. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China)
  • Online:2015-10-20 Published:2015-10-20

摘要: 【目的】通过研究共生菌 Tremblaya princeps 的形态、分布及动态变化,明确该菌在宿主扶桑绵粉蚧 Phenacoccus solenopsis体内的基本生物学特性。【方法】通过透射电镜技术观察T. princeps的超微结构;利用实时定量PCR明确T. princeps的分布和数量变化。【结果】扶桑绵粉蚧体内T. princeps的分布因性别而异。在雌虫中,T. princeps集中分布于含菌体,在其他组织器官中无分布;含菌体的大小与雌虫各个阶段的个体大小呈显著线性相关,且临近成虫期,含菌体的膨大速率加快。在雄虫中,T. princeps在个体发育的不同阶段,数量变化明显,在蛹期数量急剧增加,之后相对减少;含菌体结构在预蛹期消解。【结论】共生菌 T. princeps 的变化因宿主性别而异,且与宿主个体发育存在明显的相关性。

关键词: 扶桑绵粉蚧, Tremblaya princeps, 共生菌, 含菌体, 超微结构

Abstract: 【Aim】 This study aims to explore the characteristics of the symbiotic bacterium Tremblaya princeps in Phenacoccus solenopsis through observing its morphology, location and dynamics in different stages of hosts. 【Methods】 The ultrastructure of T. princeps was observed by transmission electron microscopy, and its location and quantitative changes were determined using real-time PCR. 【Results】 T. princeps is located in different tissues compared between females and males of the host P. solenopsis. In females, T. princeps is located only in bacteriome, but not in any other tissues or organs of the host. Size of bacteriome had a significant linear relationship with host body size, and its expanding rate accelerated as the host adults developed. In males of the host, the density of T. princeps increased dramatically before the pupal stage, and then decreased as the adult emerged. Bacteriome collapsed when the males of the host entered the pre-pupal stage. 【Conclusion】 Our results suggest that the infection status of T. princeps is varied with host sexuality and correlated to host individual development.

Key words: Phenacoccus solenopsis, Tremblaya princeps, symbiont, bacteriome, ultrastructure