褐飞虱长翅型和短翅型雌雄成虫体内磁性物质定位和定量检测

›› 2015, Vol. 58 ›› Issue (2): 132-138.

褐飞虱长翅型和短翅型雌雄成虫体内磁性物质定位和定量检测

颜学宾^1,2,#, 刘宇新^1,#, 姚亮宇^1,2, 王文肖^1,2, 潘卫东^3,*, 陈法军^1,*

(1. 南京农业大学植物保护学院昆虫学系, 南京 210095; 2. 南京农业大学资源与环境科学学院生态学系, 南京 210095; 3. 中国科学院电工研究所生物电磁学北京市重点实验室, 北京 100190)

出版日期:2015-02-20 发布日期:2015-02-20
作者简介:颜学宾, 男, 1993年生, 江苏建湖人, 学士, 主要从事生物电磁学研究, E-mail: 12211128@njau.edu.cn; 刘宇新, 男, 1983年生,山东潍坊人, 硕士研究生, 主要从事迁飞昆虫地磁定性机理研究, E-mail: 2012802140@njau.edu.cn

Localization and quantitative detection of magnetic materials in the macropterous and brachypterous female and male adults of the brown planthopper, Nilaparvata lugens(Hemiptera: Delphacidae)

YAN Xue-Bin^1,2,#, LIU Yu-Xin^1,#, YAO Liang-Yu^1,2, WANG Wen-Xiao^1,2, PAN Wei-Dong^3,*, CHEN Fa-Jun^1,*

(1. Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; 2. Department of Ecology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; 3. Beijing Key Laboratory of Bioelectromagetics, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing 100190, China)

Online:2015-02-20 Published:2015-02-20

摘要/Abstract

摘要： 【目的】地磁定向是昆虫远距离迁飞定向的重要机制之一。本研究以迁飞性害虫褐飞虱 Nilaparvata lugens 长翅型和短翅型雌雄成虫为研究对象，系统开展虫体内磁性物质定量研究。【方法】本研究利用MPMS-7型超导量子磁强计检测褐飞虱长翅型和短翅型雌雄成虫体内的磁性物质，并将经普鲁士蓝染色后的虫体超薄石蜡切片于JEM-2100型透射电子显微镜下观察磁性物质的分布状况，最后利用原子发射光谱法对虫体内的磁性物质进行定量分析与比较。【结果】超导量子磁强计检测发现，仅在褐飞虱长翅型和短翅型雌雄成虫腹部的磁滞曲线有明显闭合现象，证明该部位存在磁性物质；经透射电镜观察发现，普鲁士蓝沉淀主要呈点簇状存在于虫体腹部，证明该部位存在铁磁性物质；通过等离子发射光谱检测发现，同一性别长翅型成虫体内铁离子含量显著高于短翅型成虫，且相同翅型雄成虫体内铁离子含量显著高于雌成虫。【结论】褐飞虱长翅型和短翅型雌雄成虫腹部普遍存在铁磁性物质，且不同翅型和性别间磁性物质含量差异显著。

关键词: 褐飞虱, 迁飞定向, 翅型, 性别, 磁性物质, 超导量子磁强计, 普鲁士蓝染色, 原子发射光谱法

Abstract: 【Aim】 The geomagnetic orientation is one of the important mechanisms for the flight orientation of migratory insects in their long distance migration. In this study, the macropterous and brachypterous female and male adults of the brown planthopper (BPH), Nilaparvata lugens were selected to systemically study the magnetic materials harbored in their bodies. 【Methods】 The superconducting quantum interference device (SQID) (model: MPMS-7) was used to detect the existence of magnetic materials in the macropterous and brachypterous female and male adults of BPH, and the distribution of magnetic materials in the four types of adults was observed using the ultrathin paraffin sections (UPS) with Prussian blue staining (PBS) under the transmission electron microscope (TEM) (model: JEM-2100). The atomic emission spectroscopy (AES) was used for the quantitative analysis and comparison of the magnetic materials in the four types of adults. 【Results】 The SQID detection found that the hysteresis curves were obviously closing only in the abdomen of the macropterous and brachypterous female and male adults of N. lugens, indicating that magnetic materials harbored in the abdomen of adults. Prussian blue precipitate also mainly existed in the abdomen through the observation under TEM, indicating that the detected magnetic materials are ferromagnetic substance. There were significant differences in the contents of magnetic materials between the macropterous and brachypterous adults and between the males and females through the AES detection. For adults of the same gender, the Fe ion content in the macropterous individuals was significantly higher than that in the brachypterous individuals. And for the same wing-form, the Fe ion content in the male adults was significantly higher than that in the female adults. 【Conclusion】 Fe magnetic materials generally exist in the abdomen of macropterous and brachypterous female and male adults of N. lugens, and their contents are significanly different between the macropterous and brachypterous adults and between the males and females.

Key words: Nilaparvata lugens, migratory orientation, wing form, gender, magnetic materials, superconducting quantum interference device (SQID), Prussian blue staining (PBS), atomic emission spectroscopy (AES)

颜学宾, 刘宇新, 姚亮宇, 王文肖, 潘卫东, 陈法军. 褐飞虱长翅型和短翅型雌雄成虫体内磁性物质定位和定量检测[J]. , 2015, 58(2): 132-138.

YAN Xue-Bin, LIU Yu-Xin, YAO Liang-Yu, WANG Wen-Xiao, PAN Wei-Dong3,CHEN Fa-Jun. Localization and quantitative detection of magnetic materials in the macropterous and brachypterous female and male adults of the brown planthopper, Nilaparvata lugens(Hemiptera: Delphacidae)[J]. , 2015, 58(2): 132-138.

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