朱砂叶螨刺吸胁迫对玉米防御信号分子的诱导作用

摘要/Abstract

摘要： 【目的】探明朱砂叶螨 Tetranychus cinnabarinus 持续为害对玉米 Zea mays 叶片内茉莉酸（jasmonic acid, JA）、水杨酸（salicylic acid, SA）、乙烯（ethylene, ET）、一氧化氮（nitricoxide, NO）、脱落酸（abscisic acid, ABA）和过氧化氢（hydrogen peroxide, H₂O₂）6个防御信号分子的诱导作用。【方法】室内人工接螨（10, 20和30头/叶），采用分光光度法（SP）、高效液相色谱法(HPLC) 和酶联免疫法(ELISA)，测定了朱砂叶螨持续为害0, 24, 48, 72和96 h后，玉米幼苗叶片内6个信号分子的含量。【结果】朱砂叶螨持续刺吸为害玉米幼苗叶片后，JA, ABA和H₂O₂3个信号分子含量在叶螨刺吸为害24 h内迅速上升，在24 h时达高峰值，叶螨密度为30头/叶时其含量分别为同期未接螨对照的4.13, 3.84和3.20倍，24-48 h内迅速下降；此后，ABA和H₂O₂含量维持在较低水平，而JA含量在48-96 h内又上升至次高峰值。NO含量则在24-48 h内上升较快，48 h时达最高，叶螨密度为30头/叶时其含量为同期未接螨对照的5.09倍。SA和ET含量在96 h内均随刺吸时间的延长而增大， 96 h时最高，叶螨密度为30头/叶时其含量分别为同期未接螨对照的5.17和2.99倍。叶螨密度为30头/叶时，6个信号分子含量均显著高于同期未接螨对照（P<0.05）。【结论】朱砂叶螨为害对玉米叶片内JA, SA, ET, NO, ABA和H₂O₂均具有诱导作用，且6个信号分子在叶螨持续为害玉米叶片后循序被诱导。

关键词: 朱砂叶螨, 玉米, 防御信号分子, 茉莉酸, 水杨酸, 乙烯, 一氧化氮, 脱落酸, 过氧化氢

Abstract: 【Aim】 This study aims to investigate how infestation of Tetranychus cinnabarinus induces the generation of six defense signal molecules, including jasmonic acid (JA), salicylic acid (SA), ethylene (ET), nitricoxide (NO), abscisic acid (ABA) and hydrogen peroxide (H₂O₂) in leaves of Zea mays seedlings. 【Methods】 We measured the contents of the signal molecules in leaves of corn seedlings at 0, 24, 48, 72 and 96 h after inoculation of T. cinnabarinus (10, 20 and 30 individuals per leaf) by using spectrophotometry (SP), high-performance liquid chromatography (HPLC) or enzyme-linked immunosorbent assay (ELISA). 【Results】 Our data demonstrated that the contents of JA, ABA and H₂O₂ in leaves of corn seedlings infected by T. cinnabarinus increased rapidly within 24 h after infestation, and peaked at 24 h after inoculation of 30 individuals per leaf, which were 4.13, 3.84 and 3.20-fold as high as those of the un-inoculated control, respectively. Subsequently, the contents of JA, ABA and H₂O₂ in the infected group descended sharply from 24 to 48 h after infestation. The contents of ABA and H₂O₂ in the infected group stayed at a low level from 48 to 96 h after infestation, whereas the JAcontent in the infected group reached another peak at 96 h after infestation. The NO content in the infected group rose up from 24 to 48 h after infestation, peaked at 48 h after inoculation of 30 individuals per leaf, which was 5.09-fold as high as that of the control. The contents of SA and ET in the infected group had been increasing during the whole time of infestation, and peaked at 96 h after inoculation of 30 individuals per leaf, which were 5.17 and 2.99-fold as high as those of the control, respectively. The contents of the six signal molecules in leaves of corn seedlings infested by 30 individuals per leaf were significantly higher than those in the un-inoculated control. 【Conclusion】 The results suggest that the infestation of T. cinnabarinus induces the accumulation of JA, SA, ET, NO, ABA and H₂O₂ in leaves of corn seedlings, which are activated sequentially during infestation process.

Key words: Tetranychus cinnabarinus, Zea mays, defense signal molecules, jasmonic acid, salicylic acid, ethylene, nitricoxide, abscisic acid, hydrogen peroxide

朱玉溪, 杨群芳, 黄玉碧, 李庆, 刘应红, 蒋春先, 王海建. 朱砂叶螨刺吸胁迫对玉米防御信号分子的诱导作用[J]. , 2015, 58(6): 680-687.

ZHU Yu-Xi, YANG Qun-Fang, HUANG Yu-Bi, LI Qing, LIU Ying-Hong, JIANG Chun-Xian, WANG Hai-Jian. Induction of defense signal molecules in response to Tetranychus cinnabarinus (Acari: Tetranychidae) infestation in Zea mays[J]. , 2015, 58(6): 680-687.

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朱砂叶螨刺吸胁迫对玉米防御信号分子的诱导作用

Induction of defense signal molecules in response to Tetranychus cinnabarinus (Acari: Tetranychidae) infestation in Zea mays

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