嗜菌异小杆线虫侵染后暗黑鳃金龟和大黑鳃金龟幼虫脂肪体和中肠组织超微结构观察

›› 2015, Vol. 58 ›› Issue (8): 836-845.doi:

嗜菌异小杆线虫侵染后暗黑鳃金龟和大黑鳃金龟幼虫脂肪体和中肠组织超微结构观察

吴文丹^1，2,#, 孙昊雨^2，3,#, 席景会³, 尹姣², 张帅²,曹雅忠², 李克斌^2,*, 肖春^1,*

(1. 云南农业大学植物保护学院, 昆明 650100; 2. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193; 3. 吉林大学植物科学学院, 长春 130062)

出版日期:2015-08-20 发布日期:2015-08-20
作者简介:吴文丹, 女, 1988年生, 湖北十堰人, 硕士研究生, 研究方向为害虫防治, E-mail: sywuwdan@163.com; 孙昊雨, 男, 1987年生, 河北邢台人, 硕士研究生, 研究方向为昆虫生理生化和分子生物学, E-mail: haoyusun2006@163.com

Observations of the ultrastructure of the fat body and midgut tissues of two white grub species, Holotrichia parallela and H. oblita (Coleoptera: Melolonthidae), infected by entomopathogenic nematode Heterorhabditis bacteriophora

WU Wen-Dan^,2,#, SUN Hao-Yu^2,3,#, XI Jin-Hui³, YIN Jiao², ZHANG Shuai², CAO Ya-Zhong², LI Ke-Bin^2,*, XIAO Chun^1,*

(1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3. College of Plant Science, Jilin University, Changchun 130062, China)

Online:2015-08-20 Published:2015-08-20

摘要/Abstract

摘要： 【目的】探究昆虫病原线虫嗜菌异小杆线虫沧州品系 Heterorhabditis bacteriophora strain Cangzhou侵染对蛴螬脂肪体和中肠的影响，进一步明确其对蛴螬的致病机理。【方法】采用透射电镜技术，观察暗黑鳃金龟 Hololtrichia oblita (Faldermann)和大黑鳃金龟 H. parallela Motschulsky 2龄幼虫被嗜菌异小杆线虫沧州品系侵染后其脂肪体和中肠组织的病理变化。【结果】血腔注射感染期病原线虫嗜菌异小杆线虫沧州品系悬浮液24和48 h后，观察发现暗黑鳃金龟和大黑鳃金龟2龄幼虫脂肪体和中肠的组织结构均按时序逐渐发生变化，起初表现为脂肪球变形或变小，颜色变浅，脂肪体细胞和中肠细胞内质网、线粒体肿胀，中肠微绒毛变形脱落等现象，48 h后包裹脂肪球的膜结构破裂，脂肪体细胞和中肠细胞线粒体破裂，内质网数量减少，中肠微绒毛大量脱落，同时核内染色质大量解离，核膜破裂。【结论】经昆虫病原线虫嗜菌异小杆线虫沧州品系处理后，暗黑鳃金龟和大黑鳃金龟两种金龟甲2龄幼虫脂肪体和中肠细胞均出现明显的病理变化过程，这是嗜菌异小杆线虫高效致死蛴螬的原因之一。本研究可为昆虫病原线虫作为一种生物防治手段在蛴螬的综合防治中更好地发挥作用提供理论依据。

关键词: 昆虫病原线虫, 嗜菌异小杆线虫, 蛴螬, 暗黑鳃金龟, 大黑鳃金龟, 脂肪体, 中肠

Abstract: 【Aim】 The study aims to further explore the mechanism of pathogenicity of entomapathogenic nematodes Heterorhabditis bacteriophora strain Cangzhou infecting white grubs. 【Methods】 Transmission electron microscopy (TEM) was used to observe the morphological changes in fat body and midgut cells of the 2nd instar larvae of Hololtrichia oblita (Faldermann) and H. parallela Motschulsky, respectively. After hemocoel injection, ultrastructural changes of the white grubs were examined by TEM. 【Results】 Changes in the ultrastructure of fat body and midgut cells of the 2nd instar larvae of H. oblita and H. parallela at 24 and 48 h after injection of H. bacteriophora were observed. At 24 h after infection, fat droplets, located at fat body cells, became smaller, and their electron density became lighter in the infected larvae than those in the untreated control. Endoplasmic reticulum and mitochondria in both fat body and midgut cells became distended and mcrovilli of the midgut began to fall off in the infected larvae. At 48 h after infection, the membrane structure containing a lot of fat droplets was found broken in the infected larvae. Chromatin in fat body and midgut cell nucleus dissociated and mitochondria as well as endoplasmic reticulum were disintegrated completely in both cells of the infected larvae. Likewise, microvilli of the midgut were almost completely lost in the infected larvae. 【Conclusion】 After infection by the entomopathogenic nematode H. bacteriophora, the fat body and midgut of the 2nd instar larvae of the two white grub species, H. oblita and H. parallela, show similar pathological changes, which may be associated with the rapid death of white grubs. This study may enrich the theoretical basis for using entomopathogenic nematodes as a biological control strategy in IPM of white grubs.

Key words: Entomopathogenic nematode, Heterorhabditis bacteriophora, white grubs, Hololtrichia oblita, Holotrichia parallela, fat body, midgut

吴文丹, 孙昊雨, 席景会, 尹姣, 张帅,曹雅忠, 李克斌, 肖春. 嗜菌异小杆线虫侵染后暗黑鳃金龟和大黑鳃金龟幼虫脂肪体和中肠组织超微结构观察[J]. , 2015, 58(8): 836-845.

WU Wen-Dan, SUN Hao-Yu, XI Jin-Hui,YIN Jiao, ZHANG Shuai, CAO Ya-Zhong, LI Ke-Bin, XIAO Chun. Observations of the ultrastructure of the fat body and midgut tissues of two white grub species, Holotrichia parallela and H. oblita (Coleoptera: Melolonthidae), infected by entomopathogenic nematode Heterorhabditis bacteriophora[J]. , 2015, 58(8): 836-845.

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