荔枝蒂蛀虫转录组及嗅觉相关基因分析

doi:10.16380/j.kcxb.2016.08.003

昆虫学报 ›› 2016, Vol. 59 ›› Issue (8): 823-830.doi: 10.16380/j.kcxb.2016.08.003

荔枝蒂蛀虫转录组及嗅觉相关基因分析

孟翔^1,*, 胡俊杰², 刘慧¹, 欧阳革成¹, 郭明昉¹

（1. 广东省生物资源应用研究所, 广东省野生动物保护与利用公共实验室, 广东省农业害虫综合治理重点实验室, 广州 510260; 2. 广州大学生命科学学院, 广州 510006）

出版日期:2016-08-20 发布日期:2016-08-20

Analysis of the transcriptome and olfaction-related genes of Conopomorpha sinensis Bradley (Lepidoptera: Gracilariidae)

MENG Xiang^1,*, HU Jun-Jie2, LIU Hui¹, OUYANG Ge-Cheng¹, GUO Ming-Fang¹

(1. Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Key Laboratory of Integrated Pest Management in Agriculture, Guangdong Institute of Applied Biological Resources, Guangzhou 510260, China; 2. School of Life Sciences, Guangzhou University, Guangzhou 510006, China)

Online:2016-08-20 Published:2016-08-20

摘要/Abstract

摘要： 【目的】荔枝蒂蛀虫Conopomorpha sinensis Bradley是专一性危害我国华南地区荔枝和龙眼的重要害虫，隐蔽性强，防治困难，基因组信息缺乏。本研究的目的是获得荔枝蒂蛀虫的基因数据，寻求有效控制害虫的分子靶标。【方法】采用新一代高通量测序技术 Illumina HiSeq^TM 4000对荔枝蒂蛀虫进行转录组测序和生物信息学分析。【结果】经序列拼接获得68 996条unigenes。进一步利用七大公共数据库进行同源比对，注释了22 348 unigenes。注释到 Nr 数据库的unigenes数量最多，达27.01%，其中Nr注释的荔枝蒂蛀虫unigenes中与小菜蛾Plutella xylostella unigenes同源性最高，达34.1%。将unigenes与GO数据库比对发现，15 585条unigenes根据其功能大致可分为3类 47亚类。KEGG pathways 分析表明，7 272条unigenes 定位为267个代谢通路。基因注释进一步筛选鉴定获得100个荔枝蒂蛀虫嗅觉相关基因；与鳞翅目相关气味结合蛋白基因联合分析发现，与荔枝蒂蛀虫气味结合蛋白基因直系同源的有18 组，部分基因形成独立一簇。【结论】本研究首次获得了荔枝蒂蛀虫的转录组数据，研究结果为生物控制荔枝蒂蛀虫提供了重要的基础数据和候选分子靶标。荔枝蒂蛀虫独有的气味结合蛋白基因可能与其生境中特有的化学物质相关。

关键词: 荔枝蒂蛀虫, 转录组, 高通量测序, 基因注释, 嗅觉基因

Abstract: 【Aim】 Conopomorpha sinensis Bradley is a major and specific fruit borer pest of litchi and longan in Southern China. It is difficult to be controlled because of its better concealment, and there is a lack of its genomic information. The objective of this study is to obtain the genetic data of C. sinensis and to seek effective molecular targets for pest control. 【Methods】 The transcriptome of C. sinensis was sequenced using the Illumina HiSeq^TM 4000 sequencing platform and bioinformatics analysis. 【Results】 The clean reads were then de novo assembled into 68 996 unigenes. Through a similarity search against seven public databases, 22 348 unigenes were annotated. Most unigenes were annotated to Nr database (27.01%), and the unigenes of C. sinensis have the highest homology to those of Plutella xylostella in species distribution of unigenes in Nr database (34.1%). According to GO database, 15 585 unigenes were broadly divided into 3 categories and 47 sub-types. By searching KEGG database, 7 272 unigenes were located into 267 metabolic pathways. By further screening and identification, 100 olfaction-related genes of C. sinensis were obtained. Analysis of odorant binding protein genes from C. sinensis (CsOBPs) and the closely related species of Lepidoptera revealed that these genes form 18 OBP ortholog groups, and some form a unique cluster. 【Conclusion】 This study acquired the transcriptome data of C. sinensis for the first time. The results will provide valuable basic data and candidate molecular targets for biological control of C. sinensis. The unique CsOBPs might be associated with habitat-specific chemical components of C. sinensis.

Key words: Conopomorpha sinensis, transcriptome, high-throughput sequencing technology, gene annotation, olfactory gene

孟翔, 胡俊杰, 刘慧, 欧阳革成, 郭明昉. 荔枝蒂蛀虫转录组及嗅觉相关基因分析 [J]. 昆虫学报, 2016, 59(8): 823-830.

MENG Xiang, HU Jun-Jie, LIU Hui, OUYang Ge-Cheng, GUO Ming-Fang. Analysis of the transcriptome and olfaction-related genes of Conopomorpha sinensis Bradley (Lepidoptera: Gracilariidae)[J]. Acta Entomologica Sinica, 2016, 59(8): 823-830.

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荔枝蒂蛀虫转录组及嗅觉相关基因分析

Analysis of the transcriptome and olfaction-related genes of Conopomorpha sinensis Bradley (Lepidoptera: Gracilariidae)

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