昆虫学报 ›› 2017, Vol. 60 ›› Issue (1): 9-17.doi: 10.16380/j.kcxb.2017.01.002

• 研究论文 • 上一篇    下一篇

扶桑绵粉蚧转录组分析

胡俊杰1,*, 孟翔2, 周佳滨1, 杨陆兴1, 刘善海1, 李润钊1   

  1. (1. 广州大学生命科学学院, 广州 510006; 2. 广东省生物资源与应用研究所, 广州 510260)
  • 出版日期:2017-01-20 发布日期:2017-01-20

Transcriptome analysis of the cotton mealybug, Phenacoccus solenopsis (Hemiptera: Pseudococcidae)

HU Jun-Jie1,*, MENG Xiang2, ZHOU Jia-Bin1, YANG Lu-Xing1, LIU Shan-Hai1, LI Run-Zhao1   

  1. (1.  School of Life Sciences, Guangzhou University, Guangzhou 510006, China; 2.  Guangdong Institute of Applied Biological Resources, Guangzhou 510260, China)
  • Online:2017-01-20 Published:2017-01-20

摘要: 【目的】建立扶桑绵粉蚧Phenacoccus solenopsis Tinsley的转录组数据库,揭示扶桑绵粉蚧转录组的整体表达特征。【方法】采用Illumina HiSeqTM 4000测序平台开展对扶桑绵粉蚧雌成虫转录组测定,对原始数据进行过滤和组装。【结果】共获得58 322 258条序列读取片段(reads),共9.48 Gb(GenBank登录号: SAMN06130426)57 422 032条有效转录组数据。进一步组装拼接后,共获得94 475个单基因簇(unigene),平均长度为700 bp。将unigene与数据库中的序列进行BLASTX比对,成功注释20 949个unigenes,其中,Nr注释的unigenes与豌豆蚜Acyrthosiphon pisum unigenes同源性最高,达18.69%。扶桑绵粉蚧转录组unigenes根据GO功能注释大致可分为细胞组分、分子功能和生物过程三大类55个分支,与结合活性、催化活性、细胞进程和代谢进程相关的unigenes较多。此外,本研究还筛选到20条与脂类代谢相关的途径和与性信息素代谢相关的序列。并通过与Nr和Swiss-prot蛋白质数据库比对,获得了15 037条编码序列(CDS)片段。【结论】本研究初步阐明扶桑绵粉蚧雌成虫转录组的整体表达模式,为进一步研究扶桑绵粉蚧的基因功能及性信息素的代谢途径奠定了基础。

关键词:  , 扶桑绵粉蚧; 转录组; 基因注释; GO数据库; 编码序列; 性信息素

Abstract: 【Aim】 The objective of this study is to establish the transcriptome database of the cotton mealybug, Phenacoccus solenopsis Tinsley, and to reveal the whole expression characteristics of the data. 【Methods】 The transcriptome of female adults of P. solenopsis was sequenced using an Illumina HiSeqTM 4000 platform, and the raw data were filtered and assembled. 【Results】 A total of 58 322 258 reads containing 9.48 Gb (GenBank accession no.: SAMN06130426) valid data (57 422 032 clean reads) were obtained. A total of 94 475 unigenes with a mean length of 700 bp was obtained by further sequence splicing, and 20 949 unigenes were annotated using BLASTX searches against the databases. All unigenes against the Nr database had the highest similarity (18.69%) with those of Acyrthosiphon pisum.  Moreover, all unigenes were broadly divided into 55 branches of 3 categories (biological processes, cellular components and molecular function categories) using Gene Ontology (GO), and more unigenes were annotated to be related to metabolic process, binding activity, catalytic activity and cellular process. In addition, 20 pathways associated with lipid metabolism pathway and the sequences related to the metabolism of sex pheromone were found. Totally 15 037 coding squences (CDSs) were obtained using blast in Nr and Swiss-prot protein databases. 【Conclusion】 This study preliminarily clarified the whole expression pattern of the transcriptome of female adults of P.solenopsis, which will provide a foundation for further studying gene functions and the metabolism of sex pheromones in P.solenopsis.

Key words:  , Phenacoccus solenopsis; transcriptome; gene annotation; Gene Ontology (GO) database; coding sequence (CDS); sex pheromone