›› 2018, Vol. 61 ›› Issue (7): 843-850.doi: 10.16380/j.kcxb.2018.07.011

• 研究论文 • 上一篇    下一篇

美棘蓟马体内Wolbachia感染的时空分布

张晓晨, 冯纪年*   

  1. (西北农林科技大学, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100)
  • 出版日期:2018-07-20 发布日期:2018-07-20

Temporal and spatial distribution of Wolbachia infection in the poinsettia thrips, Echinothrips americanus (Thysanoptera: Thripidae)

ZHANG Xiao-Chen, FENG Ji-Nian*    

  1.  (Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Northwest A&F University, Yangling, Shaanxi 712100, China)
  • Online:2018-07-20 Published:2018-07-20

摘要: 【目的】明确共生菌Wolbachia在美棘蓟马Echinothrips americanus体内的时空分布动态。【方法】基于Wolbachia的外膜蛋白基因(wsp)序列设计特异引物构建标准质粒, 用绝对荧光定量PCR的方法测定美棘蓟马不同发育阶段(卵、若虫、预蛹、蛹、成虫)和雌雄成虫不同组织(头、胸、腹、腹末3节)中Wolbachiawsp基因拷贝数,分析美棘蓟马共生菌Wolbachia的感染情况。【结果】美棘蓟马体内Wolbachia 拷贝数随美棘蓟马发育进程逐渐增大,雌性成虫期Wolbachia拷贝数显著高于卵和若虫期;雌雄成虫不同组织中的拷贝数存在差异,雌性成虫腹部Wolbachia拷贝数显著高于头部、胸部和腹末3节,雄性成虫胸部和腹部Wolbachia拷贝数显著高于头部和腹末3节。美棘蓟马性别和组织与体内Wolbachia拷贝数存在显著的交互作用。【结论】本研究结果表明,美棘蓟马体内的Wolbachia感染状态不仅受宿主发育阶段的影响,而且与性别及分布位置有很大关系,为进一步了解该蓟马的发生、定植和扩散机理提供理论依据。

关键词: 美棘蓟马, Wolbachia, wsp, 时空分布, 感染密度, 绝对荧光定量PCR

Abstract: 【Aim】 To demonstrate spatio-temporal infection dynamics of Wolbachia in the poinsettia thrips, Echinothrips americanus. 【Methods】 Specific primers were designed based on the sequence of Wolbachia surface protein gene (wsp) to construct the standard plasmid. The absolute real-time quantitative PCR was used to determine the copy number of wsp in different developmental stages (egg, nymph, prepupa, pupa and adult) and adult tissues (head, thorax, abdomen and terminal three segments of abdomen) of both sexes of the thrips. 【Results】 The copy number of Wolbachia increased with the development of E. americanus, and the copy number of Wolbachia in female adult was significantly higher than those in egg and nymphal stages. The copy numbers of Wolbachia in different adult tissues were different. In female adults the copy number of Wolbachia in abdomen was significantly higher than those in head, thorax and the terminal three segments of abdomen, while in male adults the copy numbers in thorax and abdomen were significantly higher than those in head and the terminal three segments of abdomen. Sex and tissue had significant interactions with the copy number of Wolbachia in E. americanus. 【Conclusion】 The results indicate that the infection of Wolbachia in E. americanus is affected not only by the developmental stage of host, but also by host sex and tissue. This study provides a theoretical basis for understanding the occurrence, establishment and spreading of this thrips.

Key words: Echinothrips americanus, Wolbachia, wsp, spatio-temporal distribution, infection density, absolute real-time fluorescence quantitative PCR