肠道细菌弗氏柠檬酸杆菌和产酸克雷伯氏菌对斑翅果蝇生长发育和物质代谢的影响

doi:10.16380/j.kcxb.2020.04.009

摘要/Abstract

摘要： 【目的】明确弗氏柠檬酸杆菌Citrobacter freundi和产酸克雷伯氏菌Klebsiella oxytoca两种肠道共生细菌对斑翅果蝇Drosophila suzukii生长发育和物质代谢的影响。【方法】以正常饲养条件下的斑翅果蝇、构建的斑翅果蝇无菌品系以及弗氏柠檬酸杆菌和产酸克雷伯氏菌单一共生菌感染的斑翅果蝇品系为材料，检测不同品系间斑翅果蝇的卵孵化率、3龄幼虫体重和化蛹率；测定不同斑翅果蝇品系3龄幼虫体内蛋白质、氨基酸、糖原和游离脂肪酸等代谢物的含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活力。【结果】正常饲养条件下的斑翅果蝇卵孵化率、3龄幼虫体重、化蛹率及3龄幼虫体内蛋白质的含量均高于其他斑翅果蝇品系，且无菌品系中的最低。弗氏柠檬酸杆菌和产酸克雷伯氏菌感染的斑翅果蝇品系3龄幼虫中的氨基酸和糖原含量均低于斑翅果蝇无菌品系和正常品系。弗氏柠檬酸杆菌感染斑翅果蝇品系3龄幼虫体内游离脂肪酸的含量较其他品系的也降到最低。弗氏柠檬酸杆菌和产酸克雷伯氏菌感染斑翅果蝇品系3龄幼虫体内POD活力显著高于无菌品系和正常品系，而CAT活力显著低于无菌品系。【结论】斑翅果蝇肠道中无肠道共生细菌时生长发育迟缓，在食物中分别添加弗氏柠檬酸杆菌和产酸克雷伯氏菌后可一定程度上促进斑翅果蝇的发育，这与添加肠道共生菌后斑翅果蝇体内代谢物的变化密切相关。

关键词: 斑翅果蝇, 弗氏柠檬酸杆菌, 产酸克雷伯氏菌, 肠道细菌, 生长发育, 代谢物, 抗氧化酶

Abstract: 【Aim】 To understand the effect of the intestinal symbiotic bacteria Citrobacter freundi and Klebsiella oxytoca on the development and substance metabolism of the spotted wing drosophila, Drosophila suzukii. 【Methods】 The egg hatching rates, body weight of the 3rd instar larvae and pupation rates of the normal D. suzukii strain, sterile D. suzukii strain, C. freundi infected D. suzukii strain and K. oxytoca infected D. suzukii strain were determined. The contents of metabolites including protein, amino acids, glycogen and
free fatty acids and the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in the 3rd instar larvae of these D. suzukii strains were detected. 【Results】 The egg hatching rate, body weight of the 3rd instar larvae, pupation rate and
protein content in the 3rd instar larvae of the normal D. suzukii strain were all higher than those of other strains, while those of the sterile D. suzukii strain were the lowest. The 3rd instar larvae of D. suzukii strains infected by C. freundi or K. oxytoca had lower
contents of amino acids and glycogen than the sterile and normal D. suzukii strains. The contents of free fatty acids in the 3rd instar larvae of D. suzukii infected by C. freundi were also lower than those in the other D. suzukii strains. In the 3rd instar larvae of D.
suzukii strains infected by C. freundi or K. oxytoca, the POD activity was significantly higher than those in the sterile and normal strains, while the CAT activity was significantly lower than those in the sterile strain. 【Conclusion】 The development of D.
suzukii delays in the absence of intestinal symbiotic bacteria. However, the development of D. suzukii can be enhanced in some extent after adding C. freundi or K. oxytoca in the food, and this is closely related to the changes of metabolites in D. suzukii after adding
intestinal bacteria.

Key words: Drosophila suzukii; Citrobacter freundi, Klebsiella oxytoca, intestinal bacteria, growth and development, metabolites, antioxidant enzymes

高欢欢, 覃冬云, 代晓彦, 刘洁, 于毅. 肠道细菌弗氏柠檬酸杆菌和产酸克雷伯氏菌对斑翅果蝇生长发育和物质代谢的影响[J]. 昆虫学报, 2020, 63(4): 462-469.

GAO Huan-Huan, QIN Dong-Yun, DAI Xiao-Yan, LIU Jie, YU Yi . Effects of intestinal bacteria Citrobacter freundi and Klebsiella oxytoca on the development and substance metabolism of Drosophila suzukii (Diptera: Drosophilidae) [J]. Acta Entomologica Sinica, 2020, 63(4): 462-469.

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肠道细菌弗氏柠檬酸杆菌和产酸克雷伯氏菌对斑翅果蝇生长发育和物质代谢的影响

Effects of intestinal bacteria Citrobacter freundi and Klebsiella oxytoca on the development and substance metabolism of Drosophila suzukii (Diptera: Drosophilidae)

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