昆虫学报 ›› 2020, Vol. 63 ›› Issue (8): 932-940.doi: 10.16380/j.kcxb.2020.08.003

• 研究论文 • 上一篇    下一篇

伞裙追寄蝇滞育关联基因的转录组学分析

张博1, 韩海斌1, 徐林波1, 高书晶1, 甘霖2, 岳方正3, 刘爱萍1,*   

  1. (1. 中国农业科学院草原研究所, 呼和浩特 100010; 2. 阿拉善盟科技信息研究所, 内蒙古阿拉善 750399; 3. 国家林业和草原局森林和草原病虫害防治总站, 沈阳 110034)
  • 出版日期:2020-08-20 发布日期:2020-09-09

Transcriptomic analysis of diapause-associated genes in Exorista civilis (Diptera: Tachinidae)

ZHANG Bo1, HAN Hai-Bin1, XU Lin-Bo1, GAO Shu-Jing1, GAN Lin2, YUE Fang-Zheng3, LIU Ai-Ping1,*   

  1.  (1. Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Huhot 10010, China; 2. Alxa Institute of Science and Technology Information, Alxa, Inner Mongolia 750399, China; 3. General Station of Forest and Grassland Pest Management, National Forestry and Grassland Administration, Shenyang 110034, China)
  • Online:2020-08-20 Published:2020-09-09

摘要: 【目的】本研究旨在探讨调控伞裙追寄蝇Exorista civilis滞育的重要关联基因以及代谢途径,为明确该虫在转录组水平下的滞育分子机制提供理论基础。【方法】采用新一代高通量测序平台Illumina HiSeqTM2000对伞裙追寄蝇非滞育以及滞育的蛹进行转录组测序分析以及生物信息学分析;应用KAAS在线pathway比对分析工具对筛选出的满足padj<0.05且fold change≥32或padj<0.05且fold change≤1/32条件的差异表达基因进行KEGG通路富集分析。【结果】根据测序结果,共获取58 050个基因。差异倍数在32倍以上的滞育关联基因(diapause-associated genes, DAGs)有454个,其中406个表达上调,共涉及134条通路,包括氧化磷酸化、柠檬酸循环及其他重要通路;48个表达下调,涉及32条通路。KEGG通路富集分析结果表明,滞育关联基因主要集中在信号转导、内分泌系统、碳水化合物代谢等途径中。【结论】本研究获得的伞裙追寄蝇转录组数据揭示了其滞育调控的重要代谢途径与关联基因。

关键词: 伞裙追寄蝇, 转录组测序, 滞育, 滞育关联基因, KEGG分析

Abstract: 【Aim】 This study aims to explore the crucial associated genes and metabolic pathways that regulate the diapause of Exorista civilis, and to provide a theoretical basis for clarifying the molecular mechanism of diapause of this species at the transcriptome level. 【Methods】 The Illumina HiSeqTM 2000 platform was used to perform transcriptome sequencing and bioinformatics analysis of the non-diapause and diapause pupae of E. civilis. KAAS (KEGG Automatic Annotation Server) online pathway comparison tool was used to analyze the KEGG pathway enrichment of the differentially expressed genes that meet the conditions of padj<0.05 and fold change≥32 or padj<0.05 and fold change≤1/32. 【Results】 According to the sequencing results, a total of 58 050 unigenes were obtained. There were 454 diapause-associated genes (DAGs) with the difference of more than 32-fold changes. Among them, 406 DAGs were up-regulated and involved in a total of 134 pathways, including oxidative phosphorylation, citrate cycle and other important pathways, and 48 DAGs were down-regulated and related to 32 pathways. KEGG enriched pathways revealed that the DAGs are mainly involved in signal transduction, endocrine system and carbohydrate metabolism. 【Conclusion】 The transcriptome data of E. civilis obtained in this study have revealed the important metabolic pathways and associated genes in diapause regulation in E.civilis.

Key words: Exorista civilis, transcriptome sequencing, diapause, diapause-associated genes, KEGG analysis