天宫二号航天蚕后代小茧突变体sc的发现与基因定位

doi:10.16380/j.kcxb.2021.09.003

昆虫学报 ›› 2021, Vol. 64 ›› Issue (9): 1031-1040.doi: 10.16380/j.kcxb.2021.09.003

天宫二号航天蚕后代小茧突变体sc的发现与基因定位

沈广胜^1,2, 沈兴家^1,2,*, 张龙³, 赵巧玲^1,2,*, 高梦杰¹, 唐顺明^1,2, 黄静怡^1,2, 陈艳花^1,2, 蒋涛^1,2, 朱娟^1,2, 王梅仙^1,2

(1. 江苏科技大学生物技术学院, 江苏省蚕桑生物学与生物技术重点实验室, 江苏镇江 212018; 2. 中国农业科学院蚕业研究所, 农业农村部蚕桑遗传改良重点实验室, 江苏镇江 212018; 3. 中国农业大学草业科学与技术学院, 北京 100193)

出版日期:2021-09-20 发布日期:2021-09-03

Discovery of the small cocoon mutant sc of Bombyx mori traveled onboard Tiangong-2 space laboratory in space and its gene mapping#br#

SHEN Guang-Sheng^1,2, SHEN Xing-Jia^1,2,*, ZHANG Long³, ZHAO Qiao-Ling^1,2,*,GAO Meng-Jie¹, TANG Shun-Ming^1,2, HUANG Jing-Yi^1,2, CHEN Yan-Hua^1,2, JIANG Tao^1,2, ZHU Juan^1,2, WANG Mei-Xian^1,2

(1. Jiangsu Key Laboratory of Sericutural Biology and Biotechnology, College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China; 2. Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu 212018, China; 3. College of Grassland Science and Technology, China Agriculture University, Beijing 100193, China)

Online:2021-09-20 Published:2021-09-03

摘要/Abstract

摘要：

【目的】从2016年天宫二号空间实验室经历33 d后返回的1头成活“秋丰×白玉”杂交后代雌蚕Bombyx mori与地面“白玉”雄蚕交配的后代个体中，发现有结小茧突变体，进而分离并建立了飞天蚕(space silkworm)正常茧品系TG和小茧突变体品系sc。本研究通过对sc进行遗传分析和基因定位，旨在揭示产生小茧突变体的基因。【方法】对TG和sc进行表型分析；以sc, TG和正常大茧品系0223V1为试验材料，组配(sc♀×0223V1♂)F₁及回交群体BC₁F——(sc♀×0223V1♂)F₁♀×sc♂和BC₁M——sc♀×(sc♀×0223V1♂)F₁♂。以sc, 0223V1和F₁基因组DNA为模板，每个连锁群随机选10个SSR引物进行PCR扩增，筛选多态性SSR标记。利用雌性家蚕减数分裂染色体不交换的特点，用BC₁F确定sc基因所属连锁群；再根据家蚕SSR分子标记连锁图谱，用BC1M进行基因定位。【结果】表型分析表明sc幼虫体型小于TG幼虫的，蚕茧重约为TG的1/2。遗传分析表明突变受一对隐性基因sc控制；基因定位结果表明该基因位于家蚕基因组第3连锁群S2930-363和S2930-289 SSR标记之间，物理距离为684 kb，包含33个候选基因。【结论】飞天蚕小茧突变受位于家蚕基因组第3连锁群的一对隐性基因sc控制。

关键词: 家蚕, 太空诱变, 小茧, SSR标记, 基因定位

Abstract: 【Aim】 The hybrid offspring of an alive female silkworm, Bombyx mori “Qiufeng× Baiyu” brought back to the earth after travelling onboard Tiangong-2 space laboratory in space for 33 d in 2016 was mated with the male moth of “Baiyu” strain reared on the earth ’s surface for producing eggs. Small cocoon individuals were found from the offspring, and then the normal cocoon strain TG and small cocoon mutant strain sc of ‘space silkworm’ were isolated and established. The objective of this study is to reveal the gene that leads to this mutant by analyzing its inherited characters and mapping the mutant gene. 【Methods】 Phenotypic analysis of TG and sc was performed. The sc, TG and normal large cocoon strain 0223V1 were served as experimental materials for preparation of (sc♀×0223V1♂) F₁ and its backcross types, (sc♀×0223V1♂) F₁♀×sc♂ and sc♀×(sc♀×0223V1♂) F₁♂, which were designated as BC1F and BC₁M, respectively. To screen polymorphic SSR markers, the genomic DNAs of sc, 0223V1 and F₁ were used as templates, and 10 SSR primers were randomly selected from each linkage group of B. mori for PCR amplification. By utilizing the characteristic of non-crossing-over between chromosomes in female B. mori, BC₁F population was used to identify the linkage group of sc gene. According to the SSR linkage map of B. mori, BC₁M population was used for gene mapping. 【Results】 Phenotypic analysis showed that the body size of sc larvae was smaller than that of TG larvae, and the cocoon weight of sc was about half that of TG. Genetic analysis showed that the mutant was controlled by a pair of recessive gene sc. Gene mapping results showed that sc is located between S2930-363 and S2930-289 SSR markers in the 3rd linkage group of the B. mori genome, with a physical distance of 684 kb containing 33 candidate genes. 【Conclusion】 This small cocoon mutant of space silkworm is controlled by a pair of recessive gene named sc located in the 3rd linkage group of the B. mori genome.

Key words: Bombyx mori, space mutagenesis, small cocoon, SSR markers, gene mapping

沈广胜, 沈兴家, 张龙, 赵巧玲, 高梦杰, 唐顺明, 黄静怡, 陈艳花, 蒋涛, 朱娟, 王梅仙. 天宫二号航天蚕后代小茧突变体sc的发现与基因定位[J]. 昆虫学报, 2021, 64(9): 1031-1040.

SHEN Guang-Sheng, SHEN Xing-Jia, ZHANG Long, ZHAO Qiao-Ling, GAO Meng-Jie, TANG Shun-Ming, HUANG Jing-Yi, CHEN Yan-Hua, JIANG Tao, ZHU Juan, WANG Mei-Xian. Discovery of the small cocoon mutant sc of Bombyx mori traveled onboard Tiangong-2 space laboratory in space and its gene mapping#br#[J]. Acta Entomologica Sinica, 2021, 64(9): 1031-1040.

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天宫二号航天蚕后代小茧突变体sc的发现与基因定位

Discovery of the small cocoon mutant sc of Bombyx mori traveled onboard Tiangong-2 space laboratory in space and its gene mapping#br#

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