›› 1997, Vol. 40 ›› Issue (1): 45-50.

• 研究论文 • 上一篇    下一篇

保幼激素类似物对家蚕丝腺与脂肪体蛋白质合成的调节作用

戴玉锦 金绣珏   

  • 出版日期:1997-02-20 发布日期:1997-02-20

REGULATION OF JUVENILE HORMONE ANALOGUE ONPROTEIN SYNNTHESIS IN THE SILKGLAND AND FATBODY OF SILKWORM BOMBYX MORI

Dai Yujin Jin xiujue   

  • Online:1997-02-20 Published:1997-02-20

摘要: 应用示踪原子法,研究了家蚕Bombyx mori 5龄幼虫丝腺与脂肪体细胞内蛋白质合成的变化规律及保幼激素类似物(JHA738)的调节作用。从5龄初到龄末,家蚕丝腺细胞内蛋白质合成持续升高,5龄中期、后期的蛋白质合成活性分别是前期的1.60倍和2.86倍;全龄出现2个合成高峰,一个是在5龄72h,为细胞固有蛋白质合成高峰,另一个是在5龄192h,为丝蛋白合成高峰。脂肪体细胞内蛋白质合成作用呈现脉冲式的变化。在5龄前期和中期用JHA处理家蚕(剂量为4μg/条),对丝腺细胞固有蛋白质合成和脂肪体细胞蛋白质合成均表现出抑制作用,而对丝蛋白合成则表现出促进作用。本实验结果为进一步阐明JHA增丝机理提供了直接证据。

关键词: 家蚕, 丝腺, 脂肪体, 蛋白质合成, 保幼激素类似物

Abstract: We have investigated the change of protein synthesis in the silkgland and fatbody of the 5th instar larvae of Bombysc mori and the regulation of Juvenile hormone analogue (JHA738) by using the method of isotopic tracing. From the early to the late stages of the fifth instar larvae, protein synthesis in the silkgland cells of the silkworm continued to increase; the synthetic activities in the middle and late stages of fifth instar were 1.6 and 2.86 times higher than that in the early stage. Two peaksdor incorporotion of 3H-glycine occurred during the fifth instat development, the first original peak occurred at 72 h after the 4th ecdysis and the second peak for silk protein synthesis occurred at 192 h after the fourthecdysis. The protein synthesis in fat body cells displayed pulse like fluctuation. The treatment with JHA (4μglarva) at the early and middle stages of the fifth instar larvae inhibited theprotein synthesis originally in silkgland cells and fat body cells, but stimulated the incorporation of 3H-glycine into the silk protein. The silk protein synthetic activities of JHA-injected fifth-instar-larvae were higher than those of the control animals.

Key words: Bombyx mori, silkgland, fat body, protein synthesis, juvenile hormone analogue