›› 2002, Vol. 45 ›› Issue (4): 499-502.

• 研究论文 • 上一篇    下一篇


蒋国芳1, 陆敢1, 黄琨2, 黄日波2   

  • 出版日期:2002-08-20 发布日期:2002-08-20

Studies on genetic variations and phylogenetic relationships among five species of Tetrix using RAPD markers

JIANG Guo-Fang1, LU Gan1, HUANG Kun2, HUANG Ri-Bo2   

  • Online:2002-08-20 Published:2002-08-20

摘要: 用RAPD技术对蚱属5种蚱基因组DNA的多态性进行研究。在事先优化的反应条件下用12个随机引物扩增, 共得到84条清晰稳定的多态性片段,片段长度为200~2 000 bp。统计这些片段,根据扩增片段的共享度计算出相对遗传距离指数,然后用UPGMA和NJ聚类方法对其进行分析,构建系统树,确定了它们相互间的亲缘关系。

关键词: RAPD, 蚱属, 亲缘关系

Abstract: Random amplified polymorphic DNA(RAPD)techniques were applied to assess genetic variations among five species in the genus Tetrix. Amplifications with 12 primers under predetermined optimal reaction conditions gave 84 reproducible fragments ranging from 200 to 2 000 bp.The amplified products were scored as present (1) or absent (0) for each DNA sample and an index of genetic similarity (F) was calculated. The value of D was used to evaluate genetic distances between species and a phylogenetic tree was constructed.

Key words: RAPD, Tetrix, phylogenetic relationship