棉铃虫,围食膜,蛋白质组,液质联用技术,生物信息学," /> 棉铃虫,围食膜,蛋白质组,液质联用技术,生物信息学,"/> <span style="font-family:宋体;">棉铃虫围食膜的蛋白质组鉴定</span>

昆虫学报 ›› 2019, Vol. 62 ›› Issue (2): 181-192.doi: 10.16380/j.kcxb.2019.02.005

• 研究论文 • 上一篇    下一篇

棉铃虫围食膜的蛋白质组鉴定

梁振普, 王亮, 李鹏娟, 王朝兴, 肖宇博, 张小霞*   

  1.  (河南农业大学生命科学学院, 郑州 450002)

  • 出版日期:2019-02-20 发布日期:2019-02-28

Proteomic identification of the peritrophic membrane of Helicoverpa armigera (Lepidoptera: Noctuidae)

LIANG Zhen-Pu, WANG Liang, LI Peng-Juan, WANG Zhao-Xing, XIAO Yu-Bo, ZHANG Xiao-Xia*   

  1. (College of Life Sciences,HenanAgricultural University,Zhengzhou450002,China)

  • Online:2019-02-20 Published:2019-02-28

摘要:

【目的】围食膜(peritrophic membrane, PM)是昆虫抵御随食物摄入的病原微生物入侵的第一道天然屏障。本研究旨在鉴定出农业重大害虫棉铃虫Helicoverpa armigera围食膜的总蛋白成分,为进一步揭示昆虫围食膜的形成机制及研发新颖的害虫控制策略奠定基础。【方法】剥离棉铃虫5龄幼虫PM,用三氟甲磺酸(trifluoromethanesulfonic acid, TFMS)处理,采用液质联用技术(LC-MS/MS)鉴定围食膜蛋白质组,然后对鉴定结果进行生物信息学分析。【结果】本研究共鉴定出棉铃虫幼虫围食膜蛋白质169个,是目前鉴定最多的棉铃虫围食膜蛋白。通过GO分析,可以将这些鉴定的蛋白分为细胞组分、分子功能和生物学过程三大类;KEGG富集结果显示,鉴定蛋白可以富集在12条代谢通路中;蛋白互作分析(proteinprotein interaction, PPI)结果表明,以ACCCG3011等蛋白为核心可以形成蛋白互作网络。【结论】本研究鉴定了169个棉铃虫幼虫围食膜蛋白质,并对其进行了GO, KEGGPPI分析,结果有助于人们全面理解昆虫围食膜的分子结构和功能。

关键词: 棉铃虫')">棉铃虫, 围食膜')">围食膜, 蛋白质组')">蛋白质组, 液质联用技术')">液质联用技术, 生物信息学')">生物信息学

Abstract: Aim The peritrophic membrane (PM) is the first natural barrier against the invasion of microbes ingested with foods in insects. This study aims to identify the total protein constituents of the peritrophic membrane of the cotton bollworm, Helicoverpa armigera, a major agricultural insect, so as to lay a foundation for further revealing the formation mechanism of PM and developing novel pest control strategies. Methods The PMs of the 5th instar larvae of H. armigera were peeled off and then treated with trifluoromethane-sulfonic acid (TFMS). The peritrophic membrane proteome of larvae was identified by liquid chromatography and mass spectrometry (LC-MS/MS), and the identified results were subjected to bioinformatics analysis.Results In this study we identified a total of 169 peritrophic membrane proteins from H. armigera larvae, which is the highest number of PM proteins identified in H. armigera up to date. GO analysis revealed that these identified proteins could be divided into three major categories, i.e., cellular component (CC), molecular function (MF) and biological process (BP). The KEGG enrichment results showed that the identified proteins could be enriched in 12 metabolic pathways. The results of protein-protein interaction (PPI) analysis indicated that ACC and CG3011 proteins could serve as the core proteinprotein interaction network. Conclusion In this study 169 peritrophic membrane proteins were identified from H. armigera larvae and subjected to GO, KEGG and PPI analyses. The results are helpful to comprehensively understand the molecular structure and function of PM in insects.

Key words: Helicoverpa armigera; peritrophic membrane, proteome, liquid chromatography and mass spectrometry, bioinformatics