基于微卫星标记的中国枣食芽象甲地理种群遗传多样性分析

doi:10.16380/j.kcxb.2019.03.012

昆虫学报 ›› 2019, Vol. 62 ›› Issue (3): 381-390.doi: 10.16380/j.kcxb.2019.03.012

基于微卫星标记的中国枣食芽象甲地理种群遗传多样性分析

洪波^1,2, 张锋², 陈志杰², 罗坤¹, 赵惠燕^1,*

(1. 西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100; 2. 陕西省生物农业研究所, 西安 710043)

出版日期:2019-03-20 发布日期:2019-03-14

Analysis of the genetic diversity of Scythropus yasumatsui (Coleoptera: Curculionidae) populations in China based on microsatellite markers

HONG Bo^1,2, ZHANG Feng², CHEN Zhi-Jie², LUO Kun1, ZHAO Hui-Yan^1,*

(1. State Key Laboratory of Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. Bio-Agriculture Institute of Shaanxi, Xi′an 710043, China)

Online:2019-03-20 Published:2019-03-14

摘要/Abstract

摘要：

【目的】枣食芽象甲Scythropus yasumatsui是我国北方枣树Zizyphus jujuba上的一种重要的灾害性害虫，在陕西、山西、河北、河南等枣树主产区普遍发生。本研究旨在揭示我国枣食芽象甲不同种群间的遗传分化和基因交流规律。【方法】利用枣食芽象甲转录组测序的SSR序列，使用荧光标记PCR及毛细管电泳分型方法，筛选出8个微卫星位点，对我国5个省份(山西、陕西、宁夏、河北和河南)10个地理种群共308头枣食芽象甲样本进行种群遗传多样性分析。【结果】8个SSR位点均存在无效等位基因且偏离哈迪温伯格平衡。各位点的有效等位基因数(Ne)为2.113~8.016，多态信息含量(PIC)为0.561~0.908，期望杂合度(He)为0.476~0.865；种群间遗传分化系数(Fst)平均值为0.151；基因流(Nm)平均值为1.594。枣食芽象甲种群间遗传分化系数与地理距离之间显著正相关(r=0.596， P=0.0035)，基于Nei’s遗传距离和Cavalli-Sforza & Edwards余弦遗传距离的系统进化树将10个地理种群均聚为3个相同的分支。【结论】结果说明，枣食芽象甲种群遗传多样性较高，不同地理种群间存在高度的遗传分化，且有一定的基因交流；地理隔离是影响枣食芽象甲地理种群遗传分化和基因交流的重要因素。

关键词: 枣食芽象甲, 遗传多样性, 微卫星位点, 转录组, 遗传距离, 基因流

Abstract: 【Aim】Scythropus yasumatsui, a major pest of Chinese jujube (Zizyphus jujuba) trees in northern China, is widely distributed in the main producing areas of jujube in provinces including Shaanxi, Shanxi, Hebei and Henan in China. This study aims to elucidate the genetic differentiation and gene exchange among S. yasumatsui populations in China. 【Methods】 Based on simple sequence repeats (SSRs) from the previously established transcriptome database, eight SSR loci were screened by PCR with fluorescence labeling and capillary electrophoresis genotyping, and the population genetic diversity was analyzed based on 308 individuals of 10 geographic populations of S. yasumatsui from five provinces (Shanxi, Shaanxi, Ningxia, Hebei and Henan). 【Results】 All of the eight microsatellite loci had null alleles and deviated from HardyWeinberg equilibrium. Among the loci, the effective number of alleles (Ne) ranged from 2.113 to 8.016, the polymorphic information content (PIC) ranged from 0.561 to 0.908, and the expected heterozygosity (He) was between 0.476 and 0.865. The average index of genetic differentiation (Fst) among populations was 0.151, and the average gene flow (Nm) was 1.594. There was a significantly positive correlation between the genetic differentiation index and the geographic distance among different populations of S. yasumatsui (r=0.596, P=0.0035), and the phylogenetic trees based on Nei’s genetic distance and Cavalli-Sforza & Edwards genetic distance revealed that the 10 populations were all clustered into the three identical branches. 【Conclusion】 The results suggest that S. yasumatsui populations have a high level of genetic diversity, and there are a high genetic differentiation and a certain gene flow among different geographic populations. Geographic division is a critical factor which affects the genetic differentiation and gene exchange among S. yasumatsui populations.

Key words: Scythropus yasumatsui; genetic diversity, microsatellite loci, transcriptome, genetic distance, gene flow

洪波, 张锋, 陈志杰, 罗坤, 赵惠燕. 基于微卫星标记的中国枣食芽象甲地理种群遗传多样性分析[J]. 昆虫学报, 2019, 62(3): 381-390.

HONG Bo, ZHANG Feng, CHEN Zhi-Jie, LUO Kun, ZHAO Hui-Yan. Analysis of the genetic diversity of Scythropus yasumatsui (Coleoptera: Curculionidae) populations in China based on microsatellite markers[J]. Acta Entomologica Sinica, 2019, 62(3): 381-390.

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基于微卫星标记的中国枣食芽象甲地理种群遗传多样性分析

Analysis of the genetic diversity of Scythropus yasumatsui (Coleoptera: Curculionidae) populations in China based on microsatellite markers

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