乙基多杀菌素对草地贪夜蛾幼虫的毒力及对其解毒酶和乙酰胆碱酯酶活性的影响

doi:10.16380/j.kcxb.2020.05.004

昆虫学报 ›› 2020, Vol. 63 ›› Issue (5): 558-564.doi: 10.16380/j.kcxb.2020.05.004

乙基多杀菌素对草地贪夜蛾幼虫的毒力及对其解毒酶和乙酰胆碱酯酶活性的影响

高祖鹏^1,2, 郭井菲², 何康来², 王振营^2,*

(1. 吉林农业大学生物防治研究所, 天敌昆虫应用技术工程研究中心, 长春 130118; 2. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193)

出版日期:2020-05-20 发布日期:2020-06-08

Toxicity of spinetoram and its effects on the detoxifying enzyme and acetyl cholinesterase activities in Spodoptera frugiperda(Lepidoptera: Noctuidae) larvae

GAO Zu-Peng^1,2, GUO Jing-Fei², HE Kang-Lai², WANG Zhen-Ying^2,*

(1. Engineering Research Center of Natural Enemy Insects, Institute of Biological Control, Jilin Agricultural University, Changchun 130118, China; 2. State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

Online:2020-05-20 Published:2020-06-08

摘要/Abstract

摘要： 【目的】为了研究乙基多杀菌素对草地贪夜蛾Spodoptera frugiperda幼虫的毒力及作用机制。【方法】以氯虫苯甲酰胺为对照，采用表面涂抹法测定了乙基多杀菌素对草地贪夜蛾2, 3和4龄幼虫的LC₅₀和LC₉₀。采用酶联免疫吸附法(ELISA)，测定不同浓度乙基多杀菌素(0.127, 0.183, 0.250, 0.400和0.572 mg/L)处理48 h后草地贪夜蛾3龄幼虫体内多功能氧化酶(MFO)、谷胱甘肽-S-转移酶(GST)、羧酸酯酶(CarE)以及乙酰胆碱酯酶(AchE)的活性。【结果】与氯虫苯甲酰胺相比，乙基多杀菌素对草地贪夜蛾幼虫具有更高的毒力，处理48 h后对2, 3和4龄幼虫的LC₅₀值分别为0.21, 0.34和0.59 mg/L， LC₉₀值分别为0.59, 0.75和2.01 mg/L。经过乙基多杀菌素处理后，草地贪夜蛾3龄幼虫体内MFO和AchE活性均表现随着浓度的增加而显著增加，二者均在0.572 mg/L处理时活性最高，分别为52.23和23.98 U/mg pro； CarE活性在低浓度乙基多杀菌素处理(0.127和0.183 mg/L)下相对于溶剂对照(0.1% Tween-80)无显著变化，随着浓度增加至0.400与0.572 mg/L时，其活性显著增加； GST活性表现为随着乙基多杀菌素浓度增加而增加的特点, 当处理浓度为0.400与0.572 mg/L时，其活性无显著性差异。【结论】乙基多杀菌素对草地贪夜蛾幼虫的杀虫效果优于氯虫苯甲酰胺，尤其对4龄幼虫效果最为明显；在不同浓度的乙基多杀菌素处理条件下，草地贪夜蛾幼虫体内的CarE, MFO和AchE活性有所增高。

关键词: 草地贪夜蛾, 乙基多杀菌素, 毒力测定, 解毒酶, 靶标酶

Abstract: 【Aim】 The study aims to evaluate the toxicity and mechanism of action of spinetoram against the fall armyworm, Spodoptera frugiperda. 【Methods】 The LC₅₀ and LC₉₀ values of spinetoram against the 2nd, 3rd and 4th instar larvae of S. frugiperda were determined by diet-overlay bioassays with chlorantraniliprole as the control. The activities of mixed-functional oxidase (MFO), glutathione-S-transferase (GST), carboxylesterase (CarE) and acetyl cholinesterase (AchE) in the 3rd instar larvae of S. frugiperda treated by different concentrations of spinetoram (0.127, 0.183, 0.250, 0.400 and 0.572 mg/L) at 48 h post treatment were determined by enzyme-linked immunosorbent assay (ELISA). 【Results】 Compared with chlorantraniliprole, spinetoram had higher toxicity to S. frugiperda larvae. The LC₅₀ values of spinetoram against the 2nd, 3rd and 4th instar larvae at 48 h post treatment were 0.21, 0.34 and 0.59 mg/L, and the LC₉₀ values were 0.59, 0.75 and 2.01 mg/L, respectively. When the 3rd instar larvae were treated with spinetoram, the MFO and AchE activities increased significantly as the treatment concentration increased, and reached the highest (52.23 and 23.98 U/mg pro, respectively) at the concentration of 0.572 mg/L. When the 3rd instar larvae were exposed to the low concentrations of spinetoram (0.127 and 0.183 mg/L), the CarE activity showed no significant change as compared with the vehicle control (0.1% Tween-80), but significantly increased as the concentration increased to 0.400 and 0.572 mg/L. The GST activity increased significantly as the concentration of spinetoram increased, but showed no significant difference between the treatment concentrations of 0.400 and 0.572 mg/L. 【Conclusion】 Spinetoram is highly effective against S. frugiperda, especially to its 4th instar larvae, as compared with chlorantraniliprole. The activities of CarE, MFO and AchE in S. frugiperda larvae increase after exposure to different concentrations of spinetoram.

Key words: Spodoptera frugiperda, spinetoram, toxicity test, detoxification enzyme, target enzyme

高祖鹏, 郭井菲, 何康来, 王振营. 乙基多杀菌素对草地贪夜蛾幼虫的毒力及对其解毒酶和乙酰胆碱酯酶活性的影响[J]. 昆虫学报, 2020, 63(5): 558-564.

GAO Zu-Peng, GUO Jing-Fei, HE Kang-Lai, WANG Zhen-Ying . Toxicity of spinetoram and its effects on the detoxifying enzyme and acetyl cholinesterase activities in Spodoptera frugiperda(Lepidoptera: Noctuidae) larvae[J]. Acta Entomologica Sinica, 2020, 63(5): 558-564.

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乙基多杀菌素对草地贪夜蛾幼虫的毒力及对其解毒酶和乙酰胆碱酯酶活性的影响

Toxicity of spinetoram and its effects on the detoxifying enzyme and acetyl cholinesterase activities in Spodoptera frugiperda(Lepidoptera: Noctuidae) larvae

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