烟粉虱MEAM1和MED成虫在辣椒上传播番茄褪绿病毒的特性

doi:10.16380/j.kcxb.2022.11.006

昆虫学报 ›› 2022, Vol. 65 ›› Issue (11): 1452-1458.doi: 10.16380/j.kcxb.2022.11.006

烟粉虱MEAM1和MED成虫在辣椒上传播番茄褪绿病毒的特性

张吉松^1,2,3, 张卓³, 张德咏³, 张战泓⁴, 刘勇³, 史晓斌^3,*, 张友军^1,2,*

(1. 湖南农业大学植物保护学院, 长沙 410125; 2. 中国农业科学院蔬菜花卉研究所, 北京 100081; 3. 湖南省农业科学院植物保护研究所, 长沙 410125; 4. 湖南省农业科学院蔬菜研究所, 长沙 410125)

出版日期:2022-11-20 发布日期:2022-12-02

Transmission characteristics of Tomato chlorosis virus on Capsicum annuum by adult Bemisia tabaci MEAM1 and MED (Hemiptera: Aleyrodidae)

ZHANG Ji-Song^1,2,3, ZHANG Zhuo³, ZHANG De-Yong³, ZHANG Zhan-Hong⁴, LIU Yong³, SHI Xiao-Bin^3,*, ZHANG You-Jun^1,2,*

(1. College of Plant Protection, Hunan Agricultural University, Changsha 410125, China; 2. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3. Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China; 4. Institute of Vegetable, Hunan Academy of Agricultural Sciences, Changsha 410125, China)

Online:2022-11-20 Published:2022-12-02

摘要/Abstract

摘要： 【目的】分析与比较烟粉虱Bemisia tabaci MEAM1和MED成虫在辣椒Capsicum annuum植株上对番茄褪绿病毒(tomato chlorosis virus, ToCV)的传播差异，解析媒介昆虫-病毒-寄主植物间的互作关系，为田间辣椒上ToCV防治提供参考。【方法】利用烟粉虱MEAM1和MED成虫及ToCV cDNA侵染性克隆，对烟粉虱MEAM1和MED成虫在辣椒植株上获取和传播ToCV的能力进行比较，同时比较烟粉虱MEAM1和MED成虫在健康和携带ToCV辣椒植株上的取食偏好。【结果】在感染ToCV的辣椒植株上接虫后96 h内，烟粉虱MEAM1和MED成虫体内病毒积累量逐渐增加，MED成虫获毒速度和病毒积累量均大于MEAM1成虫的，MED成虫获毒量是MEAM1成虫的1.74倍；MED成虫在辣椒植株上的传毒量明显高于MEAM1成虫，传毒量平均相差9倍，且MED成虫传毒率比MEAM1成虫高29%。MEAM1和MED成虫对健康和感染ToCV的辣椒植株的取食偏好相似，都明显倾向于取食被ToCV侵染的辣椒植株。【结论】烟粉虱MED成虫在辣椒植株上获取、传播ToCV的能力均高于烟粉虱MEAM1成虫，这也是ToCV在我国大面积发生的一个重要原因。

关键词: 烟粉虱, 番茄褪绿病毒, 辣椒, 传毒, 获毒

Abstract: 【Aim】 This study aims to analyze and compare the differences in the transmission of Tomato chlorosis virus (ToCV) on Capsicum annuum plants between Bemisia tabaci MEAM1 and MED adults, and to investigate the insect-virus-host plant interaction so as to provide reference for the control of ToCV on C. annuum in the field. 【Methods】 The ToCV acquisition and transmission of B. tabaci MEAM1 and MED adults on C. annuum plants were compared by using B. tabaci MEAM1 and MED adults and cDNA infectious clones of ToCV, respectively. The feeding preference of B. tabaci MEAM1 and MED adults to healthy and ToCV-infected C. annuum plants was compared. 【Results】 When inoculated on ToCV-infected C. annuum plants for 96 h, the virus accumulation in B. tabaci MEAM1 and MED adults gradually increased, faster acquisition and higher accumulation of ToCV in MED adults than in MEAM1 adults were found, and the level of virus acquisition of MED adults was 1.74 times as high as that of MEAM1 adults. The level of virus transmission of MED adults on C. annuum plants was 10 times as high as that of MEAM1 adults, and the transmission rate of MED adults was 29% higher than that of MEAM1 adults. MEAM1 and MED adults showed similar feeding preference to healthy and ToCV-infected C. annuum plants, with an obvious feeding preference to ToCV-infected C. annuum plants. 【Conclusion】 The ability of B. tabaci MED adults to acquire and transmit ToCV on C. annuum plants is higher than that of B. tabaci MEAM1 adults, which is an important reason for the rapid spread of ToCV in China.

Key words: Bemisia tabaci; Tomato chlorosis virus; Capsicum annuum, virus transmission, virus acquisition

张吉松, 张卓, 张德咏, 张战泓, 刘勇, 史晓斌, 张友军. 烟粉虱MEAM1和MED成虫在辣椒上传播番茄褪绿病毒的特性[J]. 昆虫学报, 2022, 65(11): 1452-1458.

ZHANG Ji-Song, ZHANG Zhuo, ZHANG De-Yong, ZHANG Zhan-Hong, LIU Yong, SHI Xiao-Bin, ZHANG You-Jun. Transmission characteristics of Tomato chlorosis virus on Capsicum annuum by adult Bemisia tabaci MEAM1 and MED (Hemiptera: Aleyrodidae)[J]. Acta Entomologica Sinica, 2022, 65(11): 1452-1458.

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烟粉虱MEAM1和MED成虫在辣椒上传播番茄褪绿病毒的特性

Transmission characteristics of Tomato chlorosis virus on Capsicum annuum by adult Bemisia tabaci MEAM1 and MED (Hemiptera: Aleyrodidae)

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