携带松材线虫对松墨天牛成虫肠道和气管细菌的影响

doi:10.16380/j.kcxb.2023.10.006

昆虫学报 ›› 2023, Vol. 66 ›› Issue (10): 1319-1328.doi: 10.16380/j.kcxb.2023.10.006

携带松材线虫对松墨天牛成虫肠道和气管细菌的影响

顾宇彤^1,2, 朱浩诚^1,2, 常悦², 周娇², 樊建庭^1,*, 赵莉蔺^2,3,4,*

(1. 浙江农林大学林业与生物技术学院, 生物农药高效制备技术国家地方联合工程实验室, 杭州 311300; 2. 中国科学院动物研究所, 农业虫害鼠害综合治理研究国家重点实验室, 北京 100101; 3. 中国科学院大学, 北京 100049; 4. 中国科学院生物互作卓越创新中心, 北京 100049)

出版日期:2023-10-20 发布日期:2023-11-27

Effects of carrying Bursaphelenchus xylophilus on the bacteria in the gut and trachea of adult Monochamus alternatus (Coleoptera: Cerambycidae)

GU Yu-Tong^1,2, ZHU Hao-Cheng^1,2, CHANG Yue², ZHOU Jiao², FAN Jian-Ting^1,*, ZHAO Li-Lin^2,3,4,*

(1. National and Local Joint Engineering Laboratory for Biopesticide Efficient Preparation Technology, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China; 2. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; 3. University of Chinese Academy of Sciences, Beijing 100049, China; 4. CAS Center for Excellence in Biotic Interactions, Beijing 100049, China)

Online:2023-10-20 Published:2023-11-27

摘要/Abstract

摘要： 【目的】探究被松墨天牛Monochamus alternatus携带的松材线虫Bursaphelenchus xylophilus对松墨天牛肠道和气管细菌的影响。【方法】野外采集携带松材线虫的松墨天牛成虫后，取出完整肠道和气管进行细菌总DNA抽提后进行16S rDNA基因测序并拼接，并利用生物信息学方法分析松墨天牛成虫肠道和气管细菌组成、结构、丰度和多样性。【结果】携带松材线虫的松墨天牛成虫肠道和气管细菌菌群共检测到15门26纲66目110科201属296种，可操作分类单元(operational taxonomic unit, OTU)数目为444。携带松材线虫的松墨天牛成虫比未携带松材线虫的松墨天牛成虫肠道优势细菌菌群变化不显著，均为变形菌门(Proteobacteria)肠杆菌目(Enterobacterales)；携带松材线虫的松墨天牛成虫气管优势细菌菌群为变形菌门肠杆菌目,未携带松材线虫的松墨天牛成虫气管优势细菌菌群为厚壁菌门(Firmicutes)乳杆菌目(Lactobacillales)。携带松材线虫的松墨天牛成虫较未携带松材线虫的松墨天牛成虫气管细菌多样性和丰度升高，细菌群落结构显著不同；携带松材线虫的松墨天牛成虫肠道和气管中沙雷氏菌属Serratia和肠杆菌属Enterobacter富集。【结论】携带松材线虫后对松墨天牛成虫气管细菌菌群组成、结构、丰度和多样性都有显著影响，携带松材线虫的松墨天牛成虫气管中的沙雷氏菌属和肠杆菌属可能与松材线虫松墨天牛复合体有紧密的联系。在携带松材线虫后，松墨天牛成虫气管中沙雷氏菌属增加，可能与松墨天牛气管免疫受抑制有关。本研究为解析松墨天牛肠道和气管细菌多样性提供了理论基础，为防控松材线虫提供了新的方向。

关键词: 松墨天牛, 松材线虫, 肠道细菌, 气管细菌, 细菌多样性

Abstract: 【Aim】 To investigate the effects of Bursaphelenchus xylophilus in Monochamus alternatus on the bacteria in the gut and trachea of M. alternatus.【Methods】The whole gut and trachea were isolated from the collected M. alternatus adults carrying B. xylophilus in the field to extract DNA for sequencing and splicing of the 16S rDNA gene. The composition, structure, abundance and diversity of bacteria in the gut and trachea of M. alternatus adults carrying B. xylophilus were analyzed by bioinformatics methods. 【Results】A total of 15 phyla, 26 classes, 66 orders, 110 families, 201 genera, 296 species and 444 operational taxonomic units (OTUs) of bacterial flora in the gut and trachea of M. alternatus adults carrying B. xylophilus were detected. There was no significant change in the dominant bacterial flora in the gut between M. alternatus adults carrying B. xylophilus and M. alternatus adults without carrying B. xylophilus, and their dominant bacterial flora were Proteobacteria and Enterobacterales. Proteobacteria and Enterobacterales were the dominant bacterial flora in the trachea of M. alternatus adults carrying B. xylophilus, while Firmicutes and Lactobacillales were the dominant bacterial flora in the trachea of M. alternatus adults without carrying B. xylophilus. The diversity and abundance of bacteria in the trachea of M. alternatus adults carrying B. xylophilus were higher than those of M. alternatus adults without carrying B. xylophilus, and the bacterial community structure in the trachea of M. alternatus adults carrying B. xylophilus was significantly different from that of M. alternatus adults without carrying B. xylophilus. Serratia and Enterobacter were enriched in the gut and trachea of M. alternatus adults carrying B. xylophilus. 【Conclusion】The introduction of B. xylophilus has significant effects on the composition, structure, abundance and diversity of bacterial flora in the trachea of M. alternatus adults. Serratia and Enterobacter in the trachea of M. alternatus adults may be closely related to the B. xylophilus-M. alternatus complex. The increase of Serratia in the trachea of M. alternatus adults carrying B. xylophilus may be associated with the suppressed tracheal immunity of M. alternatus. This study provides a theoretical basis for the study of bacterial diversity in the gut and trachea of M. alternatus, and a new direction for the prevention and control of B. xylophilus.

Key words: Monochamus alternatus, Bursaphelenchus xylophilus, gut bacteria, tracheal bacteria, bacterial diversity

顾宇彤, 朱浩诚, 常悦, 周娇, 樊建庭, 赵莉蔺. 携带松材线虫对松墨天牛成虫肠道和气管细菌的影响[J]. 昆虫学报, 2023, 66(10): 1319-1328.

GU Yu-Tong, ZHU Hao-Cheng, CHANG Yue, ZHOU Jiao, FAN Jian-Ting, ZHAO Li-Lin. Effects of carrying Bursaphelenchus xylophilus on the bacteria in the gut and trachea of adult Monochamus alternatus (Coleoptera: Cerambycidae)[J]. Acta Entomologica Sinica, 2023, 66(10): 1319-1328.

[1]	陈瑾, 李慧, 胡天义, 杨华磊, 危嘉航, 金林, 郝德君. 松墨天牛成虫内生殖系统的形态与发育[J]. 昆虫学报, 2023, 66(9): 1210-1220.
[2]	潘友粮, 罗致迪, 李红征, 李金, 官忠民, 焦挺, 刘兴平. 江西境内松墨天牛生活史特征的季节和纬度变化[J]. 昆虫学报, 2023, 66(4): 522-530.
[3]	高欢欢, 赵猛, 刘利, 龙诗颖, 张安盛, 周仙红, 庄乾营. 食物中添加葡萄糖对斑翅果蝇发育与肠道细菌群落结构的影响[J]. 昆虫学报, 2023, 66(10): 1362-1373.
[4]	马微微, 徐海轩, 曹威, 闫春财, 孙泽阳. 纯水培养对重金属污染地区红裸须摇蚊幼虫肠道细菌群落多样性及潜在功能的影响[J]. 昆虫学报, 2023, 66(10): 1374-1384.
[5]	张雨丽, 曾珠, 刘艳伟, 陆俣伽, 韦伟, 鲁成, 崔为正, 闭立辉, 王平阳, 张桂征. 更换饲料对家蚕幼虫肠道细菌组成的影响及关键物种与产茧性能的相关性[J]. 昆虫学报, 2022, 65(2): 176-186.
[6]	寇若玫, 李月, 窦飞越, 周泽扬, 黄敦元. 大分舌蜂不同龄期幼虫及滞育预蛹肠道细菌群落多样性及其差异[J]. 昆虫学报, 2021, 64(6): 682-693.
[7]	陈俊贤, 周娇, 魏洪义, 赵莉蔺. 冷驯化对松墨天牛幼虫脂代谢的影响[J]. 昆虫学报, 2021, 64(12): 1433-1443.
[8]	陈昊, 罗巧玉, 马秋雨, 初旭, 袁超, 刘颖, 余伟, 吴松青, 王荣, 梁光红, 张飞萍, 胡霞. 松墨天牛漆酶基因MaLac1的克隆、原核表达及表达谱分析[J]. 昆虫学报, 2020, 63(4): 381-389.
[9]	高欢欢, 覃冬云, 代晓彦, 刘洁, 于毅. 肠道细菌弗氏柠檬酸杆菌和产酸克雷伯氏菌对斑翅果蝇生长发育和物质代谢的影响[J]. 昆虫学报, 2020, 63(4): 462-469.
[10]	李鸣宇, 周娇, 王海香, 赵莉蔺. 携带与未携带松材线虫的松墨天牛转录组差异表达基因分析[J]. 昆虫学报, 2020, 63(2): 207-217.
[11]	姜笑维, 关丹阳, 李清亚, 刘晓, Hongmei LI-BYARLAY, 贺秉军. 七氟菊酯和溴氰菊酯对棉铃虫肠道菌群的影响[J]. 昆虫学报, 2020, 63(10): 1183-1193.
[12]	孙佩璐, 崔春来, 宋红生, 王四宝. 斯氏按蚊中Toll受体参与抵抗微生物感染和调控肠道菌群稳态[J]. 昆虫学报, 2019, 62(8): 937-947.
[13]	吴晓露, 夏晓峰, 陈俊晖, Geoff M. GURR, 尤民生. 取食不同食物对小菜蛾幼虫肠道细菌多样性的影响[J]. 昆虫学报, 2019, 62(10): 1172-1185.
[14]	王立秀, 陈伟, 谢桂林, 周亮. 阿南原等跳肠道细菌的分离鉴定及降解纤维素细菌的筛选[J]. , 2018, 61(7): 835-842.
[15]	田小燕, 宋福平, 张杰, 刘荣梅, 张兴鹏, 段江燕, 束长龙. 饲喂玉米秸秆的白星花金龟幼虫肠道细菌多样性[J]. , 2017, 60(6): 632-641.

携带松材线虫对松墨天牛成虫肠道和气管细菌的影响

Effects of carrying Bursaphelenchus xylophilus on the bacteria in the gut and trachea of adult Monochamus alternatus (Coleoptera: Cerambycidae)

PDF (PC)

PDF (Mobile)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0