食物中添加葡萄糖对斑翅果蝇发育与肠道细菌群落结构的影响

doi:10.16380/j.kcxb.2023.10.010

昆虫学报 ›› 2023, Vol. 66 ›› Issue (10): 1362-1373.doi: 10.16380/j.kcxb.2023.10.010

食物中添加葡萄糖对斑翅果蝇发育与肠道细菌群落结构的影响

高欢欢^1,2, 赵猛³, 刘利², 龙诗颖¹, 张安盛¹, 周仙红¹, 庄乾营^1,*

(1. 山东省农业科学院植物保护研究所, 济南 250100; 2.山东省葡萄研究院, 济南 250100; 3. 滕州市植保检疫站, 滕州 277599)

出版日期:2023-10-20 发布日期:2023-11-27

Effects of adding glucose to foods on the development and gut bacterial community structure of Drosophila suzukii (Diptera: Drosophilidae)

GAO Huan-Huan^1,2, ZHAO Meng³, LIU Li², LONG Shi-Ying¹, ZHANG An-Sheng¹, ZHOU Xian-Hong¹, ZHUANG Qian-Ying^1,*

(1. Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2. Shandong Academy of Grape, Jinan 250100, China; 3. Tengzhou Plant Protection and Quarantine Station, Tengzhou 277599, China)

Online:2023-10-20 Published:2023-11-27

摘要/Abstract

摘要： 【目的】明确食物中添加葡萄糖对斑翅果蝇Drosophila suzukii发育及肠道细菌结构的影响，并筛选与斑翅果蝇糖代谢相关的肠道细菌种类。【方法】将添加不同浓度葡萄糖(0, 5%和10%)的食物饲喂斑翅果蝇的常规饲养品系和去除肠道微生物后的无菌品系，观察其生长发育情况以及体内葡萄糖含量的变化，并利用PacBio测序平台对各处理斑翅果蝇常规饲养品系成虫的肠道细菌进行多样性分析。【结果】与对照组相比，5%葡萄糖处理组斑翅果蝇常规饲养品系幼虫存活率和成虫羽化率分别提高60.44%和123.79%， 10%葡萄糖处理组幼虫存活率提高87.87%，但添加葡萄糖对斑翅果蝇无菌品系的发育无显著影响。无菌斑翅果蝇成虫无法利用葡萄糖，其体内葡萄糖含量明显高于常规饲养品系成虫体内的。肠道细菌多样性分析表明，斑翅果蝇常规饲养品系成虫肠道优势菌属主要为葡萄杆菌属Gluconobacter和醋杆菌属Acetobacter，食物中添加葡萄糖显著增加了肠道菌群多样性指数，醋杆菌属、普罗维登斯菌属Providencia和摩根氏菌属Morganella的丰度明显增加。斑翅果蝇常规饲养品系成虫肠道优势种为乳酸乳球菌L. lactis、弗氏葡萄杆菌G. frateurii、醋杆菌A. thailandicus和产碱普罗维斯登菌P. alcalifaciens等，在不同浓度葡萄糖浓度处理的常规饲养品系成虫中其相对丰度也存在显著差异。【结论】无菌斑翅果蝇成虫无法直接利用葡萄糖，以醋杆菌属细菌为主的肠道细菌可促进斑翅果蝇对葡萄糖的利用。本研究结果对研究肠道细菌参与斑翅果蝇营养代谢的机理提供了重要的依据。

关键词: 斑翅果蝇, 葡萄糖, 酵母, 生长发育, 肠道细菌, 糖代谢

Abstract: 【Aim】 To clarify the effects of adding glucose to foods on the development and gut bacterial community structure of Drosophila suzukii, and select the gut bacteria related to glycometabolism of D. suzulii. 【Methods】 After being fed with the artificial diets added with different concentrations of glucose (0, 5% and 10%), the growth and development of axenic (gut microbiota-free) and conventionally reared strains of D. suzukii, and the changes in the glucose content were observed, and the diversity of gut bacteria of conventionally reared D. suzukii adults was analyzed by PacBio sequencing platform. 【Results】 In the 5% glucose treatment group, the larval survival rate and adult eclosion rate of conventionally reared D. suzukii increased by 60.44% and 123.79%, respectively, and in the 10% glucose treatment group, the larval survival rate of conventionally reared D. suzukii increased by 87.87%, as compared to those in the control group. However, the development of axenic D. suzukii was not significantly affected by the addition of glucose. Axenic D. suzukii adults could not utilize glucose, leading to their higher glucose content than conventionally reared adults. The diversity analysis of gut bacteria revealed that Gluconobacter and Acetobacter were the dominant genera of gut bacteria in conventionally reared D. suzukii adults. Glucose added in foods significantly increased the diversity indexes of gut bacteria and the abundance of Acetobacter, Providencia and Morganella. The dominant species in the gut of conventionally reared D. suzukii adults were L. lactis, G. frateurii, A. thailandicus and P. alcalifaciens, and their relative abundance in D. suzukii adults exposed to different concentrations of glucose was significantly different. 【Conclusion】 Axenic D. suzukii can not utilize glucose directly. Gut bacteria (mainly Acetobacter) can promote the utilization of glucose in D. suzukii. These results provide an important basis for studying the mechanism of gut bacteria involved in nutritional metabolism in D. suzukii.

Key words: Drosophila suzukii, glucose, yeast, growth and development, gut bacteria, glycometabolism

高欢欢, 赵猛, 刘利, 龙诗颖, 张安盛, 周仙红, 庄乾营. 食物中添加葡萄糖对斑翅果蝇发育与肠道细菌群落结构的影响[J]. 昆虫学报, 2023, 66(10): 1362-1373.

GAO Huan-Huan, ZHAO Meng, LIU Li, LONG Shi-Ying, ZHANG An-Sheng, ZHOU Xian-Hong, ZHUANG Qian-Ying . Effects of adding glucose to foods on the development and gut bacterial community structure of Drosophila suzukii (Diptera: Drosophilidae)[J]. Acta Entomologica Sinica, 2023, 66(10): 1362-1373.

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食物中添加葡萄糖对斑翅果蝇发育与肠道细菌群落结构的影响

Effects of adding glucose to foods on the development and gut bacterial community structure of Drosophila suzukii (Diptera: Drosophilidae)

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