樱桃红蟑螂抗菌肽果蝇霉素基因SlDros克隆及体内诱导表达

doi:10.16380/j.kcxb.2025.07.014

昆虫学报 ›› 2025, Vol. 68 ›› Issue (7): 1023-1030.doi: 10.16380/j.kcxb.2025.07.014

樱桃红蟑螂抗菌肽果蝇霉素基因SlDros克隆及体内诱导表达

刘露遥¹, 桂航¹, 任书瑾¹, 伊丽姆努尔·玉山¹, 章冰川¹, 李飞¹, 何树林^1,2,*, 周操^1,2,*

(1. 重庆师范大学生命科学学院, 重庆 401331; 2. 重庆师范大学昆虫与分子生物学研究所, 媒介生物控制和利用重庆市重点实验室, 重庆 401331)

出版日期:2025-07-20 发布日期:2025-08-25

Cloning and in vivo induced expression of the antimicrobial peptide Drosomycin gene SlDros in Shelfordella lateralis (Blattodea: Blattidae)

LIU Lu-Yao¹, GUI Hang¹, REN Shu-Jin¹, YUSHAN Yi-Li-Mu-Nu-Er¹, ZHANG Bing-Chuan¹, LI Fei¹, HE Shu-Lin¹^,2,*, ZHOU Cao^1,2,*

(1. College of Life Sciences, Chongqing Normal University, Chongqing 401331, China; 2. Chongqing Key Laboratory of Vector Control and Utilization, Institute of Entomology and Molecular Biology, Chongqing Normal University, Chongqing 401331, China)

Online:2025-07-20 Published:2025-08-25

摘要/Abstract

摘要： 【目的】本研究旨在鉴定樱桃红蟑螂Shelfordella lateralis的抗菌肽果蝇霉素基因，并探究其发育和组织表达特性及其在细菌与杀虫剂诱导下的表达情况。【方法】基于樱桃红蟑螂转录组数据对樱桃红蟑螂的果蝇霉素基因进行鉴定，并通过RT-PCR进行验证；利用RT-qPCR测定樱桃红蟑螂不同发育阶段(1龄若虫、4龄若虫及6日龄雌成虫和雄成虫)、4龄若虫不同组织(头、胸、肠道、足、脂肪体、血淋巴和体壁)及注射大肠杆菌Escherichia coli、金黄色葡萄球菌Staphylococcus aureus和溴氰菊酯(1×^10-5 mg/L)后4龄若虫中果蝇霉素基因的表达量。【结果】克隆得到了樱桃红蟑螂果蝇霉素基因SlDros的cDNA序列(GenBank登录号: PQ464795)，其开放阅读框长201 bp，编码66个氨基酸。SlDros在樱桃红蟑螂6日龄雄成虫中大量表达；SlDros在4龄若虫头、体壁、胸和血淋巴中的表达量较高；注射大肠杆菌后12, 24和48 h时4龄若虫中SlDros的表达量显著高于对照组（注射蒸馏水）；注射金黄色葡萄球菌后6, 12, 24和72 h时4龄若虫中SlDros的表达量都显著高于对照组（注射蒸馏水）；注射溴氰菊酯96 h时4龄若虫中SlDros的表达量显著高于对照组（注射丙酮）。【结论】樱桃红蟑螂中存在抗菌肽果蝇霉素基因，并且细菌和杀虫剂胁迫均能诱导其表达。研究结果可为研究樱桃红蟑螂抗菌肽的功能及其进一步开发新型抗菌药物打下基础。

关键词: 樱桃红蟑螂, 抗菌肽, 果蝇霉素, 基因表达, 溴氰菊酯, 细菌

Abstract: 【Aim】The aim of this study is to identify antimicrobial peptide Drosomycin gene in Shelfordella lateralis, and examine the developmental and tissue expression properties and the expression of antimicrobial peptide Drosomycin gene induced by bacteria and insecticide. 【Methods】Drosomycin gene of S. lateralis was identified based on the transcriptome data of S. lateralis and further validated through RT-PCR. The expression levels of the Drosomycin gene in S. lateralis at different developmental stages (1st instar nymph, 4th instar nymph, and the 6-day-old female and male adults), in different tissues (head, thorax, gut, leg, fat body, hemolymph and integument) of the 4th instar nymphs, and in the 4th instar nymphs after injection of Escherichia coli, Staphylococcus aureus, and deltamethrin (1×10^-5 mg/L) were assayed by using RT-qPCR. 【Results】The cDNA sequence of Drosomycin gene SlDros (GenBank accession no.: PQ464795) of S. lateralis was cloned. The open reading frame of SlDros was 201 bp in length encoding 66 amino acids. SlDros was highly expressed in the 6-day-old male adults. SlDros was mainly expressed in the head, integument, thorax and hemolymph of the 4th instar nymphs. The expression levels of SlDros in the 4th instar nymphs at 12, 24 and 48 h after injection of E. coli, and those at 6, 12, 24 and 72 h after injection of S. aureus were significantly increased as compared with those of the control group injected with distilled water. At 96 h post-injection of deltamethrin, the expression level of SlDros in the 4th instar nymphs was significantly higher than that of the control group injected with acetone. 【Conclusion】The antimicrobial peptide Drosomycin gene is present in S. lateralis, and stress of bacteria or insecticide can induce its expression. These results provide a foundation for studying the function of antimicrobial peptides in S. lateralis and the further development of new antimicrobial drugs.

Key words: Shelfordella lateralis, antimicrobial peptide, drosomycin, gene expression, deltamethrin, bacteria

刘露遥, 桂航, 任书瑾, 伊丽姆努尔·玉山, 章冰川, 李飞, 何树林, 周操. 樱桃红蟑螂抗菌肽果蝇霉素基因SlDros克隆及体内诱导表达[J]. 昆虫学报, 2025, 68(7): 1023-1030.

LIU Lu-Yao, GUI Hang, REN Shu-Jin, YUSHAN Yi-Li-Mu-Nu-Er, ZHANG Bing-Chuan, LI Fei, HE Shu-Lin, ZHOU Cao. Cloning and in vivo induced expression of the antimicrobial peptide Drosomycin gene SlDros in Shelfordella lateralis (Blattodea: Blattidae)[J]. Acta Entomologica Sinica, 2025, 68(7): 1023-1030.

[1]	刘嘉睿, 孟建玉, 董祥立, 李治模, 杨昌利, 张长禹. 桃蚜热激蛋白Hsp70基因在高、低温胁迫下的表达与功能分析[J]. 昆虫学报, 2025, 68(7): 877-886.
[2]	张怡萍, 徐圣, 吴敏. 灰飞虱LWO/AC/cAMP/PKA信号通路调控细胞色素P450基因表达介导对溴氰菊酯抗性[J]. 昆虫学报, 2025, 68(5): 541-554.
[3]	张玉娟, 孙亚雯, 孟晨, 叶鸣昕, 何东林, 闫振天, 陈斌. 基于混合群体分离分析筛选中华按蚊无锡株溴氰菊酯抗性关联SNP位点及基因[J]. 昆虫学报, 2025, 68(5): 626-637.
[4]	徐淑雅, 武晓丽, 魏亚鹏, 何丽华, 董德纲, 徐锋. 乳杆菌降低家蚕抗菌肽表达[J]. 昆虫学报, 2025, 68(4): 375-385.
[5]	古丽旦·白山别克, 黄启通, 李方向, 成卫宁. 麦红吸浆虫滞育进程中细胞色素c氧化酶活力及SmCOX1和SmCOX2的表达模式分析[J]. 昆虫学报, 2025, 68(1): 14-22.
[6]	高唯漪, 蔡廷卫, 高园园, 黎成越, 任志杰, 何顺, 李建洪, 万虎. 褐飞虱和白背飞虱混合饲养中的共生细菌转移[J]. 昆虫学报, 2024, 67(5): 643-650.
[7]	焦丽亚琳, 赵萌萌, 王美, 牛越, 王西, 李锐. 星豹蛛两个GST基因克隆、鉴定及其对溴氰菊酯胁迫的响应表达[J]. 昆虫学报, 2023, 66(9): 1161-1170.
[8]	徐昌豪, 朱茜, 和易, 刘雨薇, 黄裕恬, 李睿冬, 曹三杰, 黄小波, 颜其贵, 伍锐, 文翼平, 郎一飞, 赵勤, 赵珊, 王一平, 胡雅劼, 杜森焱. 致倦库蚊成蚊吸食血餐和感染乙型脑炎病毒前后中肠细菌多样性分析[J]. 昆虫学报, 2023, 66(8): 1031-1041.
[9]	郭迎澳, 韩宝珠, 乔梁, 陈斌. 中华按蚊AsCYP9J5参与对溴氰菊酯的抗性[J]. 昆虫学报, 2023, 66(8): 1042-1051.
[10]	马倩, 董金慧, 李方向, 朱克岩, 成卫宁. 麦红吸浆虫热激蛋白基因SmHsp60的分子特征及功能研究[J]. 昆虫学报, 2023, 66(7): 859-869.
[11]	王珏, 沈东旭, 彭云武, 钱荷英, 赵巧玲, 陈安利. 抗菌肽SAAP-148对家蚕细菌性肠道病病原细菌的抗性分析[J]. 昆虫学报, 2023, 66(6): 736-746.
[12]	夏鹏亮, 严毅, 宋兴宜, 黄勇, 杨文佳. 烟草甲攻击素基因LsAttacin2的表达及其在抗细菌胁迫中的作用[J]. 昆虫学报, 2023, 66(4): 501-509.
[13]	孙彦楠, 闫锋, 高书晶, 韩海斌, 郭娜, 高海燕, 杨旭兵. 型变过程中亚洲小车蝗体内保幼激素滴度及其代谢相关基因表达量的变化[J]. 昆虫学报, 2023, 66(2): 130-137.
[14]	王汝琳, 刘向东. 灰飞虱翅型及翅发育基因对长、短翅定向选择的响应[J]. 昆虫学报, 2023, 66(2): 200-208.
[15]	静大鹏, 黄晓丹, 张天涛, 王振营. 桃蛀螟CYP6AE76基因参与对Cry1Ab蛋白的解毒作用[J]. 昆虫学报, 2022, 65(9): 1136-1143.

樱桃红蟑螂抗菌肽果蝇霉素基因SlDros克隆及体内诱导表达

Cloning and in vivo induced expression of the antimicrobial peptide Drosomycin gene SlDros in Shelfordella lateralis (Blattodea: Blattidae)

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0