Abstract: Insects are closely associated with human production and life, and therefore entomological research has important theoretical significance and practical value. Insects are good materials to study biological questions because they are easily bred in a large number. The rapid development of molecular biology techniques has greatly promoted the process of entomological research. The success of the transcriptome sequencing and gene editing technologies has broken the boundary of the model species and the non-model species, providing techniques for the study of various important agricultural pests. We need to consider the important scientific questions according to the needs of production practice and the existing theories, promote entomological research progress by using various research methods and techniques, and clarify special scientific questions in the field of entomology and common scientific questions in the field of biology as well.  

Key words:  Insect, scientific questions, methods and techniques, transcriptome, gene editing