Abstract: 【Aim】 The objective of this study is to establish the transcriptome database of the cotton mealybug， Phenacoccus solenopsis Tinsley， and to reveal the whole expression characteristics of the data. 【Methods】 The transcriptome of female adults of P. solenopsis was sequenced using an Illumina HiSeq^TM 4000 platform， and the raw data were filtered and assembled. 【Results】 A total of 58 322 258 reads containing 9.48 Gb (GenBank accession no.： SAMN06130426) valid data (57 422 032 clean reads) were obtained. A total of 94 475 unigenes with a mean length of 700 bp was obtained by further sequence splicing， and 20 949 unigenes were annotated using BLASTX searches against the databases. All unigenes against the Nr database had the highest similarity (18.69%) with those of Acyrthosiphon pisum.  Moreover， all unigenes were broadly divided into 55 branches of 3 categories (biological processes， cellular components and molecular function categories) using Gene Ontology (GO)， and more unigenes were annotated to be related to metabolic process， binding activity， catalytic activity and cellular process. In addition， 20 pathways associated with lipid metabolism pathway and the sequences related to the metabolism of sex pheromone were found. Totally 15 037 coding squences (CDSs) were obtained using blast in Nr and Swiss-prot protein databases. 【Conclusion】 This study preliminarily clarified the whole expression pattern of the transcriptome of female adults of P.solenopsis， which will provide a foundation for further studying gene functions and the metabolism of sex pheromones in P.solenopsis.

Key words:  , Phenacoccus solenopsis； transcriptome； gene annotation； Gene Ontology (GO) database； coding sequence (CDS)； sex pheromone