Acta Entomologica Sinica ›› 2017, Vol. 60 ›› Issue (4): 363-371.doi: 10.16380/j.kcxb.2017.04.001

• RESEARCH PAPERS •     Next Articles

Expression and binding characterization of odorant binding protein 1 (AcorOBP1) in Anomala corpulenta (Coleoptera: Scarabaeoidea)

WANG Chao-Qun1,2,#, ZHAO Ying2,#, CAO Ya-Zhong2, WEI Hong-Shuang2, LI Ke-Bin2, ZHANG Shuai2, PENG Yu1,*, YIN Jiao2,*   

  1. (1. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Bejing 100193, China)
  • Online:2017-04-20 Published:2017-04-20

Abstract: 【Aim】 This study aims to reveal the expression profiles of the odorant binding protein 1 (AcorOBP1) gene in Anomala corpulenta Motschulsky and its binding specificity to specific semiochemicals.【Methods】 The spatio-temporal expression profiles of AcorOBP1 in A.corpulenta was assayed by RT-qPCR. Prokaryotic expression system was used to express and purify AcorOBP1. The binding characteristics of AcorOBP1 to 56 candidate compounds were assayed by fluorescence competitive binding experiment. 【Results】 AcroOBP1 was expressed in eggs, 1st instar larvae and adults of A. corpulenta, and its  expression level in adult antenna was extremely significantly higher than those in other adult tissues (P<0.05). In the ligand-binding experiment, 53 of the 56 compounds tested were able to bind to AcorOBP1. Particularly, methyl salicylate and decyl alcohol, which are volatile components of the wax leaf privet Ligustrum quihoui, showed outstanding binding affinities to AcorOBP1 with the dissociation constants of 2.10±0.08 and 5.04±0.59 μmol/L,respectively. 【Conclusion】 AcorOBP1 shows binding affinity to a wide range of test compounds and may play a vital role in the localization of host plant L. quihoui by A. corpulenta.

Key words: Anomala corpulenta, odorant binding protein, expression profile, fluorescence competitive binding, dissociation constant