Acta Entomologica Sinica ›› 2017, Vol. 60 ›› Issue (4): 421-430.doi: 10.16380/j.kcxb.2017.04.007

• RESEARCH PAPERS • Previous Articles     Next Articles

Attractiveness of aggregation pheromones and host plant volatiles to Anoplophora glabripennis and A. chinensis (Coleoptera:Cerambycidae)

ZHU Ning1, ZHANG Dong-Yong2, WU Li-Ping3, HU Qin1, FAN Jian-Ting1,*   

  1.  (1. National Joint Engineering Laboratory for Efficient Preparation of Biopesticides, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China; 2. Zhejiang Changshan Oil-tea Research Institute, Quzhou, Zhejiang 324200, China; 3. Jiande Forest Pest Control and Quarantine Station of Zhejiang, Hangzhou 311600, China)
  • Online:2017-04-20 Published:2017-04-20

Abstract: 【Aim】 Anoplophora glabripennis and A. chinensis often coexist, endanger the common hosts and share the same aggregation pheromones. The aim of this study is to evaluate the attractiveness of aggregation pheromones and host plant volatiles to the two long-horned beetle species. 【Methods】 The indoor feeding preference of A. glabripennis and A. chinensis adults on twigs of three host species, Acer negundo Salix babylonica and Melia azedarach, was assayed by using feeding area method, and the components of host plant volatiles were analyzed by using dynamic headspace adsorption combined with gas chromatography-mass spectrometry (GC-MS). The trapping effects of aggregation pheromone and plant volatiles on these two species were investigated by field trapping tests in Yuyao and Cixi, Zhejiang. 【Results】 The results of indoor choice and no-choice feeding experiments showed that the most preferred host plant of A. glabripennis was A. negundo, followed by S. babylonica,and then M. azedarach, while the preference order of A. chinensis to host plants was just opposite: the most preferred host plant was M. azedarach, followed by S. babylonica, and then A. negundo. In the host plant volatiles, there were many terpenoids and aromatic compounds, and eachhost species had several unique volatile components and there were significant differences in the volatile components among different hostspecies. The results of the field trapping tests showed that the mixed formula MK (4-(n-heptyloxy)butan-1-ol+4-(n-heptyloxy)utanal+camphene+cis-3-hexen-1-ol+ocimene+β-caryophyllene) of the aggregation pheromones and M. azedarach volatiles, aggregation pheromone formula M (4-(n-heptyloxy)butan-1-ol+4-(n-heptyloxy)butanal), M. azedarach formula K (camphene+cis-3-hexen-1-ol+ocimene+β-caryophyllene) and S. babylonica formula L (nonanal) all trapped A. glabripennis and A. chinensis adults at the same time. Among them, the mixed formula MK of the aggregation pheromones and M. azedarach volatiles showed the best trapping effect on A. glabripennis and A. chinensis adults, and trapped more adults of both species than  aggregation pheromones or plant volatiles alone. The proportion of females trapped by aggregation pheromones was higher than the proportion of males, while the proportion of males trapped by plant volatiles was higher than that of females.【Conclusion】 The study further confirms that 4-(n-heptyloxy)butan-1-ol and 4-(n-heptyloxy)butanal are the common aggregation pheromones shared by A. glabripennis and A. chinensis. Meanwhile, nonanal, camphene, cis-3-hexen-1-ol, ocimene and β-caryophyllene are the common plant volatiles shared by A. glabripennis and A. chinensis. The combination of aggregation heromones and plant volatiles can be applied to the technology for monitoring A. glabripennis and A. chinensis in forests.

Key words: Anoplophora glabripennis, Anoplophora chinensis, feeding preference, pheromone, plant volatile, trapping