Abstract: 【Aim】 Riptortus pedestris is a major soybean pest which feeds juice in the stages of adult and nymph, causing serious damage to production. However, R. pedestris has been rarely researched, that leads to the lack of genomic resources. The objective of this study is to obtain the antennal transcriptome data of R. pedestris and to seek new methods for pest control through olfaction. 【Methods】 The adult antennal transcriptome of R. pedestris is sequenced using Illumina HiSeq^TM 4000 platform and analyzed bioinformatically. 【Results】 In total, 45 802 812 clean reads with 6.87 Gb were obtained (GenBank accession no.: SRR4429103). There were 92 259 unigenes with the mean length of 618 bp and an N50 of 1 013 bp. A total of 21 365 unigenes were annotated based on seven databases. By further analyzing the transcriptome data, we identified 219 chemoreception-related genes including 188 for olfactory receptors (ORs), 6 for gustatory receptors (GRs), 2 for ionotropic receptors (IRs), 4 for sensory neuron membrane proteins (SNMPs), 8 for odorant-binding proteins (OBPs) and 11 for chemosensory proteins (CSPs). The analysis of amino acid sequence indicated that RpedOBP1 and RpedOBP2 have three additional conserved cysteine residues immediately after the sixth cysteine and belong to the plus-C OBP family. 【Conclusion】 This study acquired the antennal transcriptom data of R. pedestris and identified olfaction-related genes. The results provide the important information data of molecular biology for the control of R. pedestris using olfaction-related gene targets.

Key words: Riptortus pedestris, antennal transcriptome, high-throughput sequencing, gene annotation, chemoreception-related genes