›› 2018, Vol. 61 ›› Issue (4): 477-487.doi: 10.16380/j.kcxb.2018.04.010

• RESEARCH PAPERS • Previous Articles     Next Articles

Analysis of the mitochondrial genome of Attagenus unicolor japonicus (Coleoptera: Dermestidae) and a phylogenetic analysis of Dermestidae

LIN Ai-Li, LI Xin-Xin, ZHAO Xin-Cheng, SONG Nan*   

  1. (College of Plant Protection, Henan Agricultural University, Zhengzhou 450000, China)
  • Online:2018-04-20 Published:2018-04-20

Abstract: 【Aim】 To analyze the mitochondrial genome of Attagenus unicolor japonicas and the phylogeny of Dermestidae. 【Methods】 The nearly complete mitochondrial genome of A. unicolor japonicus was sequenced by next-generation sequencing (NGS) method, and the phylogenetic trees were constructed using maximum likelihood method and Bayesian inference based on cox1 gene sequences of 39 insect species. 【Results】 The mitochondrial genome of A. unicolor japonicus contains 37 genes (2 ribosomal RNA genes, 22 tRNA genes and 13 protein-coding genes) and a partial control region, with the length of 14 793 bp (GenBank accession no.: MG017450). No gene rearrangement occurs in the genome organization of this insect, like most Coleoptera mitogenomes published. All protein-coding genes have typical start codon ATN except for nad1 and nad2, which start with the putative start codon TTG. cox2, nad5 and nad6 genes adjacent to the tRNAs terminate with T, while the other protein-coding genes stop with the typical stop codon TAA/TAG. The secondary structures of all tRNAs are of typical cloverleaf structure, except that of trnS1, in which the dihydrouracil arm (DHU arm) can not form a stable stem-loop structure but forms a simple loop. In addition, the anticodon for trnS1 is UCU rather than the usual GCU. The secondary structure of 16S rRNA contains five domains (domain Ⅰ, Ⅱ, Ⅳ, Ⅴ and Ⅵ) but lacks domain Ⅲ, with 44 helixes in total. The secondary structure of 12S rRNA consists of four domains and 27 helixes. The helix H47 is a highly variable region, which composes of a long stem and a big loop. The results of phylogenetic analysis showed that the relationship of Dermestidae subfamilies is ((Megatominae+Attageninae) +Dermestinae). 【Conclusion】 Dermestinae, Dermestes and Anthrenus are all monophyletic, while Trogoderma is polyphyletic. Dermestes is a sister group to the rest of Dermestidae.

Key words: Dermestidae, Attagenus unicolor japonicus, rRNA, secondary structure, mitochondrial genome, phylogeny