›› 2018, Vol. 61 ›› Issue (10): 1121-1131.doi: 10.16380/j.kcxb.2018.10.001

• RESEARCH PAPERS •     Next Articles

Identification of odorant binding protein genes in antennae of Corythucha ciliata (Hemiptera: Tingidae)

YANG Hai-Bo, HU Zhen-Jie, LI Ding-Xu, ZHU Pin-Hong, DONG Jun-Feng*   

  1.  (Forestry College, Henan University of Science and Technology, Luoyang, Henan 471000, China)
  • Online:2018-10-20 Published:2018-10-20

Abstract: 【Aim】 The sycamore lace bug, Corythucha ciliata (Hemiptera: Tingidae), is an invasive and specific forestry pest of Platanus spp. The objective of this study is to obtain the gene information of odorant binding proteins (OBPs) in C. ciliata antennae and to seek effective olfactory molecular targets for pest control. 【Methods】 The antennal transcriptomes of male and female adults of C. ciliata were sequenced using Illumina HiSeqTM 4000 platform and subjected to bioinformatics analysis. The expression patterns of OBP genes in female and male antennae of C. ciliata adults were analyzed by real-time quantitative PCR (qPCR). 【Results】 In total, 40.87 Gb clean reads were obtained from six samples of male and female antennal transcripts of C. ciliata adults, the sequences of all of the samples are more than 6.31 Gb in length. By further screening and identification, we obtained 26 putative OBP genes from C. ciliata, whose encoded proteins correspond to 24 Classic OBPs (CcilOBP1-24) and 2 Plus-C OBPs (CcilOBP25/26). Phylogenetic analysis of OBPs from C. ciliata (CcilOBPs) and other closely related Hemiptera species revealed that most CcilOBPs form a unique cluster, and a few form ortholog groups with OBPs from other hemipterous insects. qPCR analysis showed that there were significant differences in the expression levels of 11 OBP genes in the antennae of male and female adults, including 9 OBP genes (CcilOBP5/6/9/10/17/18/21/24/25) highly expressed in the male antennae, and 2 OBP genes (CcilOBP14/16) highly expressed in the female antennae. 【Conclusion】 This study acquired the molecular information of OBP genes from C. ciliata adult antennae. The results provide valuable basic data and candidate molecular targets for biological control of C. ciliata.

Key words: Corythucha ciliata, antennal transcriptome, high-throughput sequencing; odorant binding protein, realtime quantitative PCR