Acta Entomologica Sinica ›› 2020, Vol. 63 ›› Issue (11): 1358-1365.doi: 10.16380/j.kcxb.2020.11.008

• RESEARCH PAPERS • Previous Articles     Next Articles

Effects of temperature on the activities of respiratory metabolism-related and antioxidant enzymes in adults of Oedaleus asiaticus (Orthoptera: Acridoidea)

GUO Na1, GAO Shu-Jing1,*, WANG Ning1, HAN Hai-Bin1, XU Lin-Bo1, DONG Rui-Wen2Narenmanduhu2, Nabuqiya2   

  1.  (1. Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohhot 010010, China; 2. Dorbod Banner Grassland Station of Ulanqab City, Ulanqab, Inner Mongolia 011800, China)
  • Online:2020-11-20 Published:2020-12-08

Abstract: 【Aim】 To elucidate the high temperature tolerance of Oedaleus asiaticus and the effect of temperature on the activities of respiratory metabolism-related and antioxidant enzymes in O. asiaticus adults. 【Methods】 Adults of O. asiaticus were exposed to nine temperature treatments in the range of 18-42℃ at 3℃ intervals under dark conditions in light incubator for 4 h and then recovered at room temperature for 1 h. The activities of respiratory metabolismrelated enzymes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glycerol-3-phosphate dehydrogenase (GPDH), 3-hydroxyacyl-CoA dehydrogenase (HOAD) and citrate synthase (CS), and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in the adults of all the treatments were determined by biochemical method. 【Results】 The activities of the seven respiratory metabolismrelated and antioxidant enzymes in O. asiaticus adults assayed in the experiment increased first and then decreased with the temperature increasing. The activities of GAPDH, GPDH, HOAD and CS were the highest at 33, 27, 33 and 39℃, respectively, while those of SOD, CAT and POD were the highest at 30℃. The activities of all the tested seven enzymes were the lowest at 18℃. There was significant difference in the enzyme activities between female and male adults at most tested temperatures. The ratios of GAPDH activity to HOAD activity in female and male adults ranged from 1.99 to 3.31 at different temperatures, suggesting that the main carbohydrates are consumed in the respiratory metabolism of O. asiaticus. 【Conclusion】 In the tested temperature range of 18-42℃, the activities of respiratory metabolism-related enzymes and antioxidant enzymes in O. asiaticus adults first increase and then decrease with the increasing of temperature, and O. asiaticus adults adapt to temperature change by regulating the activities of respiratory metabolism-related and antioxidant enzymes.

Key words: Oedaleus asiaticus; temperature, respiratory metabolism, antioxidant enzyme; enzyme activity