Abstract:

【Aim】 This study aims to determine the species identity of one Trichogramma species recently collected in the field and to identify the types of Wolbachia in the collected Trichogramma populations in order to find more high quality species resources of Trichogramma. 【Methods】 Two atches of Trichogramma wasps were trapped by hanging up egg cards of the rice moth, Corcyra cephalonica, in the Arboretum of South China Agricultural University. Combined with the morphological identification and the molecular identification method through PCR amplification, sequencing and analysis of ITS2 sequence, the species of Trichogramma materials collected in the field were identified. Wolbachia infection in the Trichogramma wasps was detected by PCR amplification of the outer membrane protein gene (wsp) sequence in Wolbachia. The homology analysis of the detected Wolbachia in the Trichogramma wasps was carried out by PCR amplification of wsp and multilocus sequence typing (MLST). 【Results】 All the two batches of Trichogramma wasps collected in this study were identified as Trichogramma oleae Voegelé & Pointel, in which the infection rate of Wolbachia was 100%. The results showed that Wolbachia in T. oleae belongs to the Sib subgroup in the B supergroup, corresponding to MLST sequence type ST486, having the close relationship with the Wolbachia in T. oleae (former Yugoslavia line), T. pretiosum(Uruguay line) and T.deion (Netherlands line). 【Conclusion】 This is the first record of T. oleae captured in the wild in China, which is a parthenogenetic strain with complete infection of Wolbachia. This study provides a new natural enemy species resource for pest biological control and a valuable research material for further study on the interaction between Wolbachia and Trichogramma.

Key words: Trichogramma oleae, male external genitalia, ITS2, Wolbachia, MLST, phylogeny, China