Abstract: 【Aim】 To clarify the defense mechanism of Ostrinia furnacalis against cadmium and chlorantraniliprole, and to evaluate the cumulative effects of heavy metals and pesticide pollution on insects. 【Methods】 The newly hatched larvae of O. furnacalis were fed with the artificial diet containing 5 mg/kg cadmium, 0.003 mg/kg chlorantraniliprole and 5 mg/kg cadmium+0.003 mg/kg chlorantraniliprole, respectively, and those fed with the normal artificial diet were used as the control. The developmental duration, body weight, adult emergence rate and abnormal pupal rate were recorded, and the activities of detoxification enzymes including glutathione-S-transferase (GST), carboxylesterase (CarE) and acetylcholinesterase (AChE) in O. furnacalis at different developmental stages (3rd instar larva, female and male pupae, and female and male adults) were detected. 【Results】 Cadmium (5 mg/kg), chlorantraniliprole (0.003 mg/kg), and their combined treatment (5 mg/kg cadmium+0.003 mg/kg chlorantraniliprole) had effects on the growth and development of O. furnacalis. In chlorantraniliprole treatment group, the larval duration of O. furnacalis was the longest, significantly prolonged by 7.1 d as compared to that in the control group, and the pupal weight, adult weight and adult emergence rate of O. furnacalis were the lowest, significantly decreased by 9.1 mg, 2.4 mg and 25.5% as compared with those in the control group. In cadmium+chlorantraniliprole treatment group, the larval duration of O. furnacalis was 4.9 d shorter than that in chlorantraniliprole treatment group, and the abnormal pupal rate was the highest, significantly increased by 19.4% as compared to that in the control group. The adult duration of O. furnacalis in cadmium+chlorantraniliprole treatment group was the shortest, significantly reduced by 1.6 d as compared to that in the control group. Cadmium, chlorantraniliprole and cadmium+chlorantraniliprole had effects on the detoxification enzyme activities in O. furnacalis. Compared with the control, cadmium, chlorantraniliprole and cadmium+chlorantraniliprole showed inducing effects on the GST activities in the 3rd instar larvae, female and male pupae and male adults of O. furnacalis, and the GST activities in different developmental stages of O. furnacalis in cadmium+chlorantraniliprole treatment group were significantly higher than those in the other treatment groups and the control group. Compared with the control, cadmium, chlorantraniliprole and cadmium+chlorantraniliprole showed inhibitory effects on the CarE activities in different developmental stages of O. furnacalis, and the cadmium+chlorantraniliprole treatment had the strongest inhibitory effect on the CarE activities in various developmental stages of O. furnacalis. Compared with the control, cadmium, chlorantraniliprole and cadmium+chlorantraniliprole showed inducing effects on the AChE activity in the 3rd instar larvae of O. furnacalis, but had different effects on the AChE activities in the pupae and adults. 【Conclusion】 Cadmium (5 mg/kg), chlorantraniliprole (0.003 mg/kg) and their combined treatment can all affect the growth and development of O. furnacalis, and have different effects on its detoxification enzyme activities.

Key words: Ostrinia furnacalis, cadmium, chlorantraniliprole, growth and development, detoxification enzymes