嗜卷书虱Cu/Zn-SOD1, Cu/Zn-SOD2和Fe/Mn-SOD的克隆及对高低温胁迫的响应

doi:10.16380/j.kcxb.2024.01.001

Abstract

Abstract:

【Aim】 To reveal the role of the superoxide dismutase genes in Liposcelis bostrychophila in response to high and low temperature stresses. 【Methods】The cDNAs of three superoxide dismutase genes Cu/Zn-SOD1, Cu/Zn-SOD2 and Fe/Mn-SOD of L. bostrychophila were cloned by RT-PCR, and their sequence characteristics were analyzed by bioinformatics methods. RT-qPCR was used to detect the relative expression levels of Cu/Zn-SOD1, Cu/Zn-SOD2 and Fe/Mn-SOD in adults at 0, 1 and 2 h under high temperature (42 ℃) and low temperature (4 ℃) stresses. 【Results】 LbCu/Zn-SOD1, LbCu/Zn-SOD2 and LbFe/Mn-SOD (GenBank accession numbers are OQ938782, OQ938783 and OQ938784, respectively) of L. bostrychophila were cloned, with the open reading frames (ORFs) of 465, 630 and 636 bp in length, encoding 154, 209 and 211 amino acids with the relative molecular weights of 15.85, 22.33 and 23.72 kD, and the isoelectric points of 6.17, 7.68 and 6.79, respectively. LbCu/Zn-SOD1 and LbCu/Zn-SOD2 had one and two Cu/Zn superoxide dismutase signatures, respectively. LbFe/Mn-SOD had one Fe/Mn superoxide dismutase signature. The results of phylogenetic analysis showed that Cu/Zn-SOD1, Cu/Zn-SOD2 and Fe/Mn-SOD were highly conserved in insects. The expression of LbCu/Zn-SOD1, LbCu/Zn-SOD2 and LbFe/Mn-SOD in adults of L. bostrychophila was induced by high temperature42 ℃ stress, and the expression levels of LbCu/Zn-SOD1 and LbCu/Zn-SOD2 inadults of L. bostrychophila were significantly higher than that of the control at 1 and 2 h. The expression level of LbFe/Mn-SOD in adults of L. bostrychophila was significantly lower than that of the control at 1 h and significantly higher than that of the control at 2 h under42 ℃stress. There was no significant difference in the expression levels of LbCu/Zn-SOD1 and LbCu/Zn-SOD2 inadults of L. bostrychophila at 1 h, while that of LbFe/Mn-SOD decreased significantly at 1 h and those of LbCu/Zn-SOD1, LbCu/Zn-SOD2 and LbFe/Mn-SOD increased significantly at 2 h under 4 ℃stress as compared with that of the control. 【Conclusion】 The superoxide dismutase genes LbCu/Zn-SOD1, LbCu/Zn-SOD2 and LbFe/Mn-SOD are involved in the tolerance of L. bostrychophila to extreme temperature stress.

Key words: Liposcelis bostrychophila')">Liposcelis bostrychophila, superoxide dismutase, gene cloning, expression ana1ysis
')">temperature stress; expression ana1ysis

WANG Xiao, XU De-Jun, ZHU Bin-Jian, XU Jun-Ting, AO Guo-Hong, ZHANG Chang-Yu, HAN Kai-Yu.

Cloning of Cu/Zn-SODl, Cu/Zn-SOD2 and Fe/Mn-SOD in Liposce1is bostrychophilα (Psocoptera: Liposce1ididae) and their responseto high and1ow temperature stresses [J].Acta Entomologica Sinica, 2024, 67(1): 1-8.

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URL: http://www.insect.org.cn/EN/10.16380/j.kcxb.2024.01.001

http://www.insect.org.cn/EN/Y2024/V67/I1/1

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Cloning of Cu/Zn-SODl, Cu/Zn-SOD2 and Fe/Mn-SOD in Liposce1is bostrychophilα (Psocoptera: Liposce1ididae) and their responseto high and1ow temperature stresses

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