Abstract: 【Aim】To explore the effects of artificial diet-based rearing technology on the growth, development and reproduction of Anoplophora glabripennis, to clarify the duration and reproductive parameters of A. glabripennis at different developmental stages, and to establish a system of indoor pass-on technology of A. glabripennis based on artificial diet, so as to provide basic data and technical support for the in-depth basic research on A. glabripennis and the development of new preventive and control technologies in the future. 【Methods】 The duration and mortality rates of different developmental stages of A. glabripennis reared on artificial diet were observed and counted. The effects of feeding different host plants (Salix babylonica and Acer negundo branches) on the adult longevity and number of eggs laid by female adult of A. glabripennis were observed and compared to clarify the feeding preference of A. glabripennis adults for different host plants, and to explore the host plant species suitable for indoor nutrient supplementation of A. glabripennis adults. The differences in the adult longevity and number of eggs laid per female of A. glabripennis of different sources (reared on artificial diet indoors and collected in the field) to evaluate the effects of different growing environments on the development and breeding biology of adult A. glabripennis. The pupal weight of A. glabripennis reared on artificial diet indoors and collected in the field was determined to clarify the effects of different growing environments on the pupal weight of A. glabripennis. 【Results】 Under the condition of the temperature 25 ℃, relative humidity of 60% and photoperiod of 16L∶8D, the egg duration of A. glabripennis was (10.39±0.09) d, and the entire larval duration was (153.78±5.93) d. The larval stage of A. glabripennis reared on artificial diet indoors was divided into five instars, and the 1st-5th instar larval duration was (18.28±0.23), (24.75±0.20), (33.30±0.27), (37.67±0.27) and (39.85±0.31) d, respectively. The pupal duration of A. glabripennis was (15.05±0.06) d, and the survival rates of various developmental stages were maintained at more than 90.0%. Nutrient supplementation with A. negundo branches significantly increased the number of eggs laid per female and adult longevity of A. glabripennis, with the average values of (112.97±3.64) grains, (39.90±4.69) d and (30.93±6.75) d, respectively, which were significantly higher than those of females with nutrient supplemention with S. babylonica branches ［(26.33±0.97) grains, (15.23±3.41) d and (15.50±4.35) d, respectively］. In comparison, there were no significant differences in the number of eggs laid per female and female and male adult longevity of A. glabripennis reared on artificial diet indoors ［(101.50±34.17) grains, (41.00±3.82) d, and (30.50±8.23) d, respectively］ and those collected in the field ［(94.25±18.59) grains, (38.63±6.12) d and (25.88±6.60) d, respectively］. In addition, the pupal weight of A. glabripennis reared on artificial diet indoors was (0.95±0.20) g, which was not significantly different from that collected in the field ［(0.93±0.12) g］. 【Conclusion】 The artificial diet rearing method in this study can not only greatly improve the survival rate of artificially bred A. glabripennis, but also achieve the indoor 2 generations/year pass.on rearing, which greatly shortens the breeding cycle of A. glabripennis, and can provide a stable source of test worms for the basic research of A. glabripennis, and lays a foundation for further development of new prevention and control technologies.

Key words:  Anoplophora glabripennis, artificial diet, developmental duration, adult longevity, fecundity, pupal weight